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Mandelstam–Brillouin Light Scattering in Bovine Serum Albumin Solutions with Different Concentrations in the Vicinity of Thermal Denaturation

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Abstract

Phase transformations of bovine serum albumin (BSA) are investigated using the Brilluoin light scattering in the temperature range from 300 to 380 K in solutions with concentrations of 50 and 5 mg/mL. As the reference sequence, the sequence of phase transformations in a concentrated (100 mg/mL) BSA solution are used for analyzing the results. It is shown that the sequence of BSA phase transformations is modified upon a decrease in the concentration. For example, in the vicinity of denaturation, the region with fibrilform protein aggregates disappears, and the properties of gel-type high-temperature phase change.

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Authors and Affiliations

  1. Ioffe Institute, 194021, St. Petersburg, Russia

    A. V. Dmitriev, A. I. Fedoseev & S. G. Lushnikov

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  1. A. V. Dmitriev
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  2. A. I. Fedoseev
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  3. S. G. Lushnikov
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Correspondence to S. G. Lushnikov.

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The authors declare that they have no conflicts of interest.

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Translated by N. Wadhwa

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Dmitriev, A.V., Fedoseev, A.I. & Lushnikov, S.G. Mandelstam–Brillouin Light Scattering in Bovine Serum Albumin Solutions with Different Concentrations in the Vicinity of Thermal Denaturation. Tech. Phys. 65, 1546–1550 (2020). https://doi.org/10.1134/S1063784220100059

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  • DOI: https://doi.org/10.1134/S1063784220100059

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