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Simulation of the type of coralin alkaloid-DNA binding

Abstract

Interaction between a synthesized coralin protoberberine alkaloid and the DNA double helix of the calf’s thymus in a salt solution is studied by optical absorption spectroscopy and spectropolarimetry. The dependence of the spectral characteristics of the alkaloid on a ratio between the DNA base pair concentration and the alkaloid molecule concentration is considered. The parameters of bonds between the coralin alkaloid and the DNA double helix are determined using modified McGhee-von Hippel equations.

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Correspondence to K. G. Kulikov.

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Original Russian Text © K.G. Kulikov, T.V. Koshlan, 2015, published in Zhurnal Tekhnicheskoi Fiziki, 2015, Vol. 85, No. 5, pp. 5–11.

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Kulikov, K.G., Koshlan, T.V. Simulation of the type of coralin alkaloid-DNA binding. Tech. Phys. 60, 639–644 (2015). https://doi.org/10.1134/S1063784215050096

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  • DOI: https://doi.org/10.1134/S1063784215050096

Keywords

  • Alkaloid
  • Circular Dichroism
  • Scatchard Plot
  • Aqueous Salt Solution
  • Optical Absorption Spectroscopy