Skip to main content
SpringerLink
Log in

Diagnostics of living cells under an atomic force microscope using a submicron spherical probe with a calibrated radius of curvature

  • Experimental Instruments and Technique
  • Published:
Technical Physics Aims and scope Submit manuscript

Abstract

The relief and mechanical elasticity of living cells from lines A549 and L41 are studied. To minimize the destructive effect of the atomic force microscope on the cell and improve the measuring accuracy of the elasticity module, special probes with a single colloid amorphous SiO2 nanogranule at their tips are employed. A procedure for preparing such probes is demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. Binnig, C. F. Quate, and C. Gerber, Phys. Rev. Lett. 56, 930 (1968).

    Article  ADS  Google Scholar 

  2. V. L. Mironov, Fundamentals of Scanning Probe Microscopy (IFM RAN, Nizhnii Novgorod, 2004).

    Google Scholar 

  3. H.-J. Butt, E. K. Wolff, S. A. C. Gould, B. Dixon Northern, C. M. Peterson, and P. K. Hansma, J. Struct. Biol. 105, 54 (1990).

    Article  Google Scholar 

  4. W. Häberle, J. K. H. Hörber, and G. Binnig, J. Vac. Sci. Technol. B 9, 1210 (1991).

    Article  Google Scholar 

  5. F. M. Ohnesorge, H. K. H. Horber, W. Haberle, C.-P. Czerny, D. P. E. Smith, and G. Binnig, Biophys. J. 73, 2183 (1997).

    Article  Google Scholar 

  6. Y. L. Wang and D. E. Discher, Methods in Cell Biology: Cell Mechanics (Elsevier, California, 2007), Vol. 83, pp. 347–372.

    Google Scholar 

  7. D. Mazia, G. Schatten, and W. Sale, Cell Biol. 66, 198 (1975).

    Article  Google Scholar 

  8. S. Kasas and A. Ikai, Biophys. J. 68, 1678 (1995).

    Article  ADS  Google Scholar 

  9. D. Alsteens, V. Dupres, K. McEvoy, L. Wildling, H. J. Gruber, and Y. F. Dufrene, Nanotechnology 19, 384005 (2008).

    Article  ADS  Google Scholar 

  10. http://www.ntmdt-tips.com/products/view/csg01; http://probe.olympus-global.com/en/product/biolever.cfm

  11. H.-J. Butt and M. Jaschke, Nanotechnology 6, 1 (1995).

    Article  ADS  Google Scholar 

  12. T. G. Kuznetsova, M. N. Starodubtseva, N. I. Yegorenkov, S. A. Chizhik, and R. I. Zhdanov, Micron 38, 824 (2007).

    Article  Google Scholar 

  13. P. Jena and J. K. H. Horber, Methods in Cell Biology: Atomic Force Microscopy (Elsevier, San Diego, 2002), Vol. 68, pp. 67–90.

    Google Scholar 

  14. J. Domke, W. J. Parak, M. George, H. E. Gaub, and M. Radmacher, Eur. Biophys. J. 28, 179 (1999).

    Article  Google Scholar 

  15. E. Henderson, P. G. Haydon, and D. S. Sakaguchi, Science 257, 1944 (1992).

    Article  ADS  Google Scholar 

  16. C. Rotsch and M. Radmacher, Biophys. J. 78, 520 (2000).

    Article  ADS  Google Scholar 

  17. Yu. A. Vasil’ev, Sorosov. Obraz. Zh., No. 4, 4 (1996).

  18. S. Schaus and E. Henderson, Biophys. J. 73, 1205 (1997).

    Article  Google Scholar 

  19. P. K. Hansma, B. Drake, O. Marti, S. A. Gould, and C. B. Prater, Science 243(4891), 641 (1989).

    Article  ADS  Google Scholar 

  20. Y. E. Korchev, C. L. Bashford, M. Milovanovic, I. Vodyanoy, and M. J. Lab, Biophys. J. 73, 653 (1997).

    Article  Google Scholar 

  21. J. Rheinlaender, N. A. Geisse, R. Proksch, and T. E. Schaffer, Langmuir 27, 697 (2011).

    Article  Google Scholar 

  22. I. Sokolov, Atomic Force Microscopy in Cancer Cell Research. Cancer Nanotechnology, Ed. by H. S. Nalwa, and T. Webster (American Scientific, Valencia, 2007), pp. 1–17.

    Google Scholar 

  23. C. Spagnoli, A. Beyder, S. Besch, and F. Sachs, Phys. Rev. E 78, 031916 (2008).

    Article  ADS  Google Scholar 

  24. L. H. Mak, M. Knoll, D. Weiner, A. Gorschluter, A. Schirmeisen, and H. Fuchs, Rev. Sci. Instrum. 77, 046104 (2006).

    Article  ADS  Google Scholar 

  25. Yang Gan, Rev. Sci. Instrum. 78, 081101 (2007).

    Article  ADS  Google Scholar 

  26. A. A. Sominina, Methodological Recommendations for Working with Cell Cultures and Media (VNII Grippa MZ SSSR, Moscow, 1975).

    Google Scholar 

  27. L. B. Piotrovskii, M. Yu. Eropkin, E. M. Eropkin, M. A. Dumpis, and O. I. Kiselev, Psikhofarmakol. Biol. Narkol., No. 7, 15458 (2007).

  28. M. Yu. Eropkin, L. B. Piotrovskii, E. M. Eropkina, M. A. Dumpis, E. V. Litasova, and O. I. Kiselev, Eksp. Klinich. Farmakol. 74, 28 (2011).

    Google Scholar 

  29. http://www.spmtips.com28/main/

  30. http://microspheres-nanospheres.com

  31. W. Stöber, A. Fink, and E. Bohn, J. Colloid Interface Sci. 26, 62 (1968).

    Article  Google Scholar 

  32. E. Yu. Trofimova, A. E. Aleksenskii, S. A. Grudinkin, I. V. Korkin, D. A. Kurdyukov, and V. G. Golubev, Kolloid. Zh., No. 73, 535 (2011).

  33. http://www.germanairbruch.com/

  34. http://www.fixme.ru/products/ritelok/uv.html

  35. A. San Paulo and R. Garc’a, Phys. Rev. B 64, 193411 (2001).

    Article  ADS  Google Scholar 

  36. J. E. Sader, J. Appl. Phys. 84, 64 (1998).

    Article  ADS  Google Scholar 

  37. B. Bhushan, Handbook of Micro/Nanotribiology, 2nd ed. (CRC, Boca Raton, 1999).

    Google Scholar 

  38. S. Sh. Rekhviashvili, B. A. Rozenberg, and V. V. Dremov, JETP Lett. 88, 772 (2008).

    Article  ADS  Google Scholar 

  39. http://www.ntmdt-tips.com/products/view/tgt1

  40. I. A. Nyapshaev, A. V. Ankudinov, and A. P. Voznyakovskii, Phys. Solid State 53, 1882 (2011).

    Article  ADS  Google Scholar 

  41. E. K. Dimitriadis, F. Horkay, J. Maresca, B. Kachar, and R. S. Chadwick, Biophys. J. 82, 2798 (2002).

    Article  Google Scholar 

  42. F. Rico, P. Roca-Cusachs, N. Gavara, R. Farré, M. Rotger, and D. Navajas, Phys. Rev. E 72, 921914 (2005).

    Article  ADS  Google Scholar 

  43. R. Chen, T. A. Ratnikova, M. B. Stone, S. Lin, M. Lard, G. Huang, J. S. Hudson, and P. C. Ke, Small 6, 612 (2010).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ioffe Physical Technical Institute, Russian Academy of Sciences, Politekhnicheskaya ul. 26, St. Petersburg, 194021, Russia

    I. A. Nyapshaev, A. V. Ankudinov & E. Yu. Trofimova

  2. St. Petersburg National Research University of Information Technologies, Mechanics, and Optics, Kronverkskii pr. 49, St. Petersburg, 197101, Russia

    A. V. Ankudinov & A. V. Stovpyaga

  3. Research Institute of Influenza, Ministry of Health and Social Development of the Russian Federation, ul. Prof. Popova 15/17, St. Petersburg, 197376, Russia

    M. Yu. Eropkin

Authors
  1. I. A. Nyapshaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Ankudinov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. V. Stovpyaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Yu. Trofimova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Yu. Eropkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Ankudinov.

Additional information

Original Russian Text © I.A. Nyapshaev, A.V. Ankudinov, A.V. Stovpyaga, E.Yu. Trofimova, M.Yu. Eropkin, 2012, published in Zhurnal Tekhnicheskoi Fiziki, 2012, Vol. 82, No. 10, pp. 109–116.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nyapshaev, I.A., Ankudinov, A.V., Stovpyaga, A.V. et al. Diagnostics of living cells under an atomic force microscope using a submicron spherical probe with a calibrated radius of curvature. Tech. Phys. 57, 1430–1437 (2012). https://doi.org/10.1134/S1063784212100167

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1063784212100167

Keywords

Search

Navigation