Plasma Physics Reports

, Volume 44, Issue 1, pp 125–136 | Cite as

Contribution to the Chemistry of Plasma-Activated Water

  • J. JulákEmail author
  • A. Hujacová
  • V. Scholtz
  • J. Khun
  • K. Holada
Low-Temperature Plasma


Plasma-activated water (PAW) was prepared by exposure to nonthermal plasma produced by a positive dc corona discharge in a transient spark regime. The activation of water was performed in atmosphere of various surrounding gases (air, nitrogen, carbon dioxide, and argon). This PAW retains its biological activity, measured on the mouse neuroblastoma cells culture, even after storage for more than one year. The highest hydrogen peroxide content was found for PAWs prepared in the atmospheres of argon or carbon dioxide, whereas the PAWs prepared in air and nitrogen exhibited lower hydrogen peroxide content. The acidity of PAWs mediated by nitric and nitrous acid formation displayed an opposite trend. It is concluded that the long-lasting biological effect of PAW is mediated by hydrogen peroxide in acid milieu only, whereas other possible active components decompose rapidly.


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • J. Julák
    • 1
    Email author
  • A. Hujacová
    • 1
  • V. Scholtz
    • 2
  • J. Khun
    • 2
  • K. Holada
    • 1
  1. 1.Institute of Immunology and Microbiology, First Faculty of MedicineCharles University and General University HospitalPraha 2Czech Republic
  2. 2.Department of Physics and MeasurementsUniversity of Chemistry and TechnologyPraha 6Czech Republic

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