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High-frequency underwater plasma discharge application in antibacterial activity

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Abstract

Plasma discharge is a novel disinfection and effectual inactivation approach to treat microorganisms in aqueous systems. Inactivation of Gram-negative Escherichia coli (E. coli) by generating high-frequency, high-voltage, oxygen (O2) injected and hydrogen peroxide (H2O2) added discharge in water was achieved. The effect of H2O2 dose and oxygen injection rate on electrical characteristics of discharge and E. coli disinfection has been reported. Microbial log reduction dependent on H2O2 addition with O2 injection was observed. The time variation of the inactivation efficiency quantified by the log reduction of the initial E. coli population on the basis of optical density measurement was reported. The analysis of emission spectrum recorded after discharge occurrence illustrated the formation of oxidant species (OH•, H, and O). Interestingly, the results demonstrated that O2 injected and H2O2 added, underwater plasma discharge had fabulous impact on the E. coli sterilization. The oxygen injection notably reduced the voltage needed for generating breakdown in flowing water and escalated the power of discharge pulses. No impact of hydrogen peroxide addition on breakdown voltage was observed. A significant role of oxidant species in bacterial inactivation also has been identified. Furthermore the E. coli survivability in plasma treated water with oxygen injection and hydrogen peroxide addition drastically reduced to zero. The time course study also showed that the retardant effect on E. coli colony multiplication in plasma treated water was favorable, observed after long time. High-frequency underwater plasma discharge based biological applications is technically relevant and would act as baseline data for the development of novel antibacterial processing strategies.

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Authors and Affiliations

  1. Department of Nuclear and Energy Engineering, Jeju National University, Jeju, Republic of Korea

    M. W. Ahmed, S. Choi, K. Lyakhov, U. Shaislamov, R. Suresh & H. J. Lee

  2. Faculty of Biotechnology, Jeju National University, Jeju, Republic of Korea

    R. K. Mongre & D. K. Jeong

Authors
  1. M. W. Ahmed
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  2. S. Choi
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  3. K. Lyakhov
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  4. U. Shaislamov
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  7. R. Suresh
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  8. H. J. Lee
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Correspondence to H. J. Lee.

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Ahmed, M.W., Choi, S., Lyakhov, K. et al. High-frequency underwater plasma discharge application in antibacterial activity. Plasma Phys. Rep. 43, 381–392 (2017). https://doi.org/10.1134/S1063780X17030011

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  • DOI: https://doi.org/10.1134/S1063780X17030011

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