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Crystallization and X-Ray Diffraction Studies of Salmonella Invasion Protein D (SipD), Insight Tip Component of Salmonella typhi Type III Secretion System

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Abstract

Many pathogenic gram-negative bacteria including Salmonella typhi utilize type III secretion systems (T3SSs) to inject bacterial proteins into host cells and to initiate infections. One of the essential components of the T3SS is the tip protein known as SipD, translocon component via the type III secretion system SPI-1. Here, recombinant SipD is produced and crystallized; the details of cloning, expression, and crystallization are reported. Crystals of SipD are obtained by sitting-drop vapour diffusion method. X-ray diffraction data are collected up to 3.8 Å resolution. Single crystals belong to the monoclinic sp. gr. P2; unit-cell parameters: a = 70.86, b = 103.70, c = 89.28 Å, β = 107.63°.

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ACKNOWLEDGMENTS

Authors are grateful to Dr. K. Gunasekaran, Assistant professor, Centre of Advanced Study in Crystallography and Biophysics, Guindy Campus, University of Madras, Chennai, India, for manuscript preparation

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Authors and Affiliations

  1. Structural Biology Laboratory, Department of Bioinformatics, Bharathiar University, Coimbatore, 641046, Tamilnadu, India

    Gopinath Samykannu & Sundarabaalaji Narayanan

  2. Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India

    Perumal Perumal, Raina Rahul & Arockiasamy Arulandu

Authors
  1. Gopinath Samykannu
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  2. Perumal Perumal
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  3. Raina Rahul
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  4. Arockiasamy Arulandu
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  5. Sundarabaalaji Narayanan
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Correspondence to Arockiasamy Arulandu.

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Gopinath Samykannu, Perumal, P., Rahul, R. et al. Crystallization and X-Ray Diffraction Studies of Salmonella Invasion Protein D (SipD), Insight Tip Component of Salmonella typhi Type III Secretion System. Crystallogr. Rep. 64, 1108–1111 (2019). https://doi.org/10.1134/S1063774519070174

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  • DOI: https://doi.org/10.1134/S1063774519070174

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