Crystallography Reports

, Volume 62, Issue 3, pp 364–369 | Cite as

Small-angle X-ray scattering study of conditions for the formation of growth units of protein crystals in lysozyme solutions

  • Yu. A. Dyakova
  • K. B. Ilina
  • P. V. Konarev
  • A. E. Kryukova
  • M. A. Marchenkova
  • A. E. Blagov
  • V. V. Volkov
  • Yu. V. Pisarevsky
  • M. V. Kovalchuk
Diffraction and Scattering of Ionizing Radiations

Abstract

The structural composition of lysozyme solutions favorable for the formation of the tetragonal form of protein crystals was studied by synchrotron-based small-angle X-ray scattering depending on the protein concentration and the temperature. Along with lysozyme monomers, dimers and octamers are found in crystallization solutions; the octamer content increases with an increase in the protein concentration.

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Copyright information

© Pleiades Publishing, Inc. 2017

Authors and Affiliations

  • Yu. A. Dyakova
    • 1
    • 2
  • K. B. Ilina
    • 1
    • 2
  • P. V. Konarev
    • 1
    • 2
  • A. E. Kryukova
    • 1
    • 2
  • M. A. Marchenkova
    • 1
    • 2
  • A. E. Blagov
    • 1
    • 2
  • V. V. Volkov
    • 1
  • Yu. V. Pisarevsky
    • 1
    • 2
  • M. V. Kovalchuk
    • 1
    • 2
    • 3
  1. 1.Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”Russian Academy of SciencesMoscowRussia
  2. 2.National Research Centre “Kurchatov Institute”MoscowRussia
  3. 3.St. Petersburg State UniversitySt. PetersburgRussia

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