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Crystallization and preliminary X-ray diffraction study of porcine carboxypeptidase B

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Abstract

Crystals of porcine pancreatic carboxypeptidase B have been grown in microgravity by the capillary counter-diffusion method through a gel layer. The X-ray diffraction study showed that the crystals belong to sp. gr. P41212 and have the following unit-cell parameters: a = b = 79.58 Å, c = 100.51 Å; α = β = γ = 90.00°. The X-ray diffraction data set suitable for the determination of the three-dimensional structure at atomic resolution was collected from one of the grown crystals at the SPring 8 synchrotron facility to 0.98 Å resolution.

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Authors and Affiliations

  1. Scientific Center of Russian Federation Research Institute for Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi proezd 1, Moscow, 113545, Russia

    V. Kh. Akparov

  2. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow, 119333, Russia

    V. I. Timofeev & I. P. Kuranova

  3. National Research Centre “Kurchatov Institute”, pl. Akademika Kurchatova 1, Moscow, 123098, Russia

    V. I. Timofeev & I. P. Kuranova

Authors
  1. V. Kh. Akparov
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  2. V. I. Timofeev
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  3. I. P. Kuranova
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Correspondence to V. Kh. Akparov.

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Original Russian Text © V.Kh. Akparov, V.I. Timofeev, I.P. Kuranova, 2015, published in Kristallografiya, 2015, Vol. 60, No. 3, pp. 413–415.

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Akparov, V.K., Timofeev, V.I. & Kuranova, I.P. Crystallization and preliminary X-ray diffraction study of porcine carboxypeptidase B . Crystallogr. Rep. 60, 367–369 (2015). https://doi.org/10.1134/S1063774515030025

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  • DOI: https://doi.org/10.1134/S1063774515030025

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