Advertisement

Crystallography Reports

, Volume 59, Issue 1, pp 112–116 | Cite as

Formation of the long-periodicity phase in model membranes of the outermost layer of skin (Stratum corneum)

  • M. A. KiselevEmail author
  • E. V. Ermakova
  • A. Yu. Gruzinov
  • A. V. Zabelin
Liquid Crystals

Abstract

Model SC membranes (lipid membranes fabricated from the main components of the lipid matrix of the outermost layer of mammalian skin (stratum corneum (SC)) are used to study the general regularities of the nanostructurization of SC lipid matrix. A model membrane from the outermost layer of skin SC with a composition ceramide 1/ceramide 6/cholesterol/palmitic acid/cholesterol sulfate and a component weight ratio of 30/30/20/15/5 has been investigated by X-ray diffraction. It is shown that the membrane structure at pH = 7.2 consists of two short-periodicity phases with repeat distances d = 47 and 35.7 Å and a long-periodicity phase with d = 127 Å. An increase in pH to 9.0 leads to destruction of the long-periodicity phase, while the short-periodicity phase is retained with d = 48.3 Å.

Keywords

Stratum Corneum DPPC Crystallography Report Lipid Matrix Repeat Distance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. V. Rawlings and P. J. Matts, J. Invest. Dermatol. 124, 1099 (2005).CrossRefGoogle Scholar
  2. 2.
    Y. Mizutani, S. Mitsutake, K. Tsuji, et al., Biochimie 91, 784 (2009).CrossRefGoogle Scholar
  3. 3.
    G. C. Charalambopoulou, T. A. Steriotis, T. Hauss, et al., J. Appl. Phys. A 74, 1245 (2002).ADSCrossRefGoogle Scholar
  4. 4.
    J. Bouwstra, G. Pilgram, G. Gooris, et al., Skin Pharmacol. Appl. Skin Physiol. 14, 52 (2001).CrossRefGoogle Scholar
  5. 5.
    N. Ohta, S. Ban, H. Tanaka, et al., Chem. Phys. Lipids 123, 1 (2003).CrossRefGoogle Scholar
  6. 6.
    T. McIntosh, Biophys J. 85, 1675 (2003).ADSCrossRefGoogle Scholar
  7. 7.
    D. C. Swartzendruber, P. W. Wertz, D. J. Kitko, et al., J. Invest. Dermatol. 92, 251 (1989).CrossRefGoogle Scholar
  8. 8.
    J. R. Hill and P. W. Wertz, Biochem. Biophys. Acta 16, 121 (2003).CrossRefGoogle Scholar
  9. 9.
    D. Groen, G. S. Gooris, and J. A. Bouwstra, Biophys. J. 97, 2242 (2009).ADSCrossRefGoogle Scholar
  10. 10.
    A. Schroeter, D. Kessner, M. A. Kiselev, et al., Biophys. J. 97, 1104 (2009).ADSCrossRefGoogle Scholar
  11. 11.
    M. A. Kiselev, N. Yu. Ryabova, A. M. Balagurov, et al., Eur. Biophys. J. 34, 1030 (2005).CrossRefGoogle Scholar
  12. 12.
    A. Ruettinger, M. A. Kiselev, Th. Hauss, et al., Eur. Biophys. J. 37, 759 (2008).CrossRefGoogle Scholar
  13. 13.
    A. Schroeter, M. A. Kiselev, Th. Hauss, et al., Biochem. Biophys. Acta 1788, 2194 (2009).CrossRefGoogle Scholar
  14. 14.
    D. Kessner, M. Kiselev, S. Dante, et al., Eur. Biophys. J. 37, 989 (2008).CrossRefGoogle Scholar
  15. 15.
    M. A. Kiselev, Crystallogr. Rep. 52(3), 529 (2007).ADSCrossRefGoogle Scholar
  16. 16.
    M. A. Kiselev, E. V. Ermakova, N. Yu. Ryabova, et al., Crystallogr. Rep. 55(3), 466 (2010).ADSCrossRefGoogle Scholar
  17. 17.
    V. N. Korneev, P. M. Sergienko, A. M. Matyushin, et al., Nucl. Instrum. Methods Phys. Res. A 543, 368 (2005).ADSCrossRefGoogle Scholar
  18. 18.
    M. J. Ruocco and G. G. Shipley, Biochem. Biophys. Acta 691, 309 (1982).CrossRefGoogle Scholar
  19. 19.
    M. A. Kiselev, T. Gutberlet, and P. Lesieur, Chem. Phys. Lipids 133, 181 (2005).CrossRefGoogle Scholar
  20. 20.
    S. Raudenkolb, S. Wartewig, and R. H. H. Neubert, Chem. Phys. Lipids 133, 89 (2005).CrossRefGoogle Scholar
  21. 21.
    J. Zbytovska, M. A. Kiselev, S. S. Funari, et al., Colloids Surf. A: Physicochem. Eng. Aspects 328, 90 (2008).CrossRefGoogle Scholar
  22. 22.
    H. S. Shieh, L. G. Hoard, and C. E. Nordman, Nature 267, 287 (1977).ADSCrossRefGoogle Scholar
  23. 23.
    D. Bach and E. Wachtel, Biochem. Biophys. Acta 1610, 187 (2003).CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2014

Authors and Affiliations

  • M. A. Kiselev
    • 1
    Email author
  • E. V. Ermakova
    • 1
  • A. Yu. Gruzinov
    • 2
  • A. V. Zabelin
    • 2
  1. 1.Joint Institute for Nuclear ResearchDubna, Moscow oblastRussia
  2. 2.National Research Centre “Kurchatov Institute”MoscowRussia

Personalised recommendations