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Estimation of the effect of relative humidity on protein crystallization

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Abstract

The effect of relative humidity in a crystallization box on the rate of establishment of supersaturation conditions during protein crystallization by diffusion of solvent vapors is estimated. A modified crystallization box is designed, which provides the formation of a stable air flow with a specified relative humidity and its measurement directly in the closed space between a drop and a reservoir. The range of relative humidities necessary to obtain the supersaturation conditions in a drop with a protein crystallization solution is determined.

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References

  1. T. M. Bergfors, Protein Crystallization: Techniques, Strategies, and Tips (International University Line, La Jolla, 1999).

    Google Scholar 

  2. A. McPherson, Crystallization of Biological Macromolecules (CSHL Press, New York, 1999).

    Google Scholar 

  3. G. T. Detitta and J. R. Luft, Acta Crystallogr. D 51, 786 (1995).

    Article  Google Scholar 

  4. J. R. Luft, D. Albright, et al., Acta Cristallogr. D 52, 1098 (1996).

    Article  Google Scholar 

  5. W. Eames, N. J. Marr, and H. Sabir, Int. J. Heat Mass Transfer 40, 2963 (1997).

    Article  Google Scholar 

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Authors and Affiliations

  1. Yerevan State University, Yerevan, 375025, Armenia

    T. G. Hudaverdyan

  2. Max Planck Unit for Structural Molecular Biology, Hamburg, 22607, Germany

    G. S. Kachalova & H. D. Bartunik

  3. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, 142290, Russia

    G. S. Kachalova

Authors
  1. T. G. Hudaverdyan
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  2. G. S. Kachalova
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  3. H. D. Bartunik
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Additional information

Original Russian Text © T.G. Hudaverdyan, G.S. Kachalova, H.D. Bartunik, 2006, published in Kristallografiya, 2006, Vol. 51, No. 3, pp. 554–559.

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Hudaverdyan, T.G., Kachalova, G.S. & Bartunik, H.D. Estimation of the effect of relative humidity on protein crystallization. Crystallogr. Rep. 51, 519–524 (2006). https://doi.org/10.1134/S1063774506030242

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  • DOI: https://doi.org/10.1134/S1063774506030242

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