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The Hematological Parameters of the Black Scorpionfish Scorpaena porcus Linnaeus, 1758 Under Experimental Hypothermia in vivo

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Abstract

The linear dimensions of erythrocytes, their nuclei, and the number of cells and hemoglobin in the blood of the black scorpionfish Scorpaena porcus Linnaeus, 1758 were studied in the autumn–winter period, at water temperatures of 17, 13, 8, and 4°С. The results showed high and relatively equal hemoglobin contents and the number of erythrocytes in the blood of black scorpionfish at 17 and 13°C. However, the studied parameters at a water temperature of 13°С were taken as the physiological norm, since this temperature more corresponded with the autumn temperatures of the Karadag coast. Lowering the water temperature in the aquarium to 8°C reduced the hemoglobin level by 25% and the number of erythrocytes by 26% in the blood of S. porcus. The linear dimensions of erythrocytes did not change when the temperature dropped to 8°C. At a temperature of 4°C the number of erythrocytes and hemoglobin in the blood of S. porcus rapidly decreased by 57% and by 31%, respectively, and swelling of erythrocytes was observed. The large diameter of red blood cells increased by 11% and the small diameter by 25%. The linear dimensions of the nuclei of erythrocytes increased by 12% along the major and minor axes at a temperature of 8°С. At 4°C, the nuclei of erythrocytes decreased in size, along the major axis by 7%, and along the minor axis by 9%, indicating a disruption of the chromatin structure in the nuclear apparatus of the cell. The results we obtained made it possible to carry out a clear gradation of the temperature resistance of S. porcus. At water temperatures below 8°С, destructive processes occurred in the blood, indicating the exhaustion of protective reserves in this species. The data we obtained can be used in fish mariculture as indicators of resistance to temperature factors.

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Funding

This work was supported by ongoing institutional funding of the Kovalevsky Institute of Biology of the Southern Seas within the framework of the theme of state program no. 075-00744-21-00 Study of fundamental physical, physiological and biochemical, reproductive, population and behavioral characteristics of marine hydrobionts. No additional grants to carry out or direct this particular research were obtained.

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  1. Vyazemsky Karadag Scientific Station, Federal Research Center, Institute of Biology of Southern Seas, 298187, Feodosia, Russia

    Yu. A. Silkin, E. N. Silkina, M. Yu. Silkin & V. E. Vasilets

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  1. Yu. A. Silkin
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  3. M. Yu. Silkin
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Correspondence to Yu. A. Silkin.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

Animal-related experiments (fish) were conducted in accordance with the Guidelines of the execution of works during fishing in research and control purposes for 2022–2024 Comprehensive studies of aquatic bioresources and their habitat in the Azov-Black Sea basin (Black and Azov Seas) based on the Order Rosrybolovstvo no. 793 dated December 28, 2022.

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Translated by N. Pehr

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Silkin, Y.A., Silkina, E.N., Silkin, M.Y. et al. The Hematological Parameters of the Black Scorpionfish Scorpaena porcus Linnaeus, 1758 Under Experimental Hypothermia in vivo. Russ J Mar Biol 50, 33–42 (2024). https://doi.org/10.1134/S1063074024010085

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