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A Comparative Morphometric Analysis of the Azov Anchovy (Engraulis encrasicolus maeoticus Pusanov, 1926) and the Black Sea Anchovy (E. encrasicolus ponticus Alexandrov, 1927) Based on Body Shape

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Abstract

A comparative analysis of body shape was performed on 841 specimens of anchovy collected from 21 catches from the Sea of Azov, the Kerch Strait, and the Black Sea off Crimea and the northeastern coast of the Caucasus in 2006–2007 and 2016–2017. Twenty-three measurements between 12 homologous landmarks of the body of each specimen according to the Truss Network System served as characters for analysis. Fifteen characters showed significant differences between the Azov anchovy Engraulis encrasicolus maeoticus Pusanov, 1926 and the Black Sea anchovy E. encrasicolus ponticus Alexandrov, 1927. Specimens of the Azov subspecies had larger relative values for all characters of the head region, the height of the trunk region, the relative length of the bases of the dorsal and anal fins, and the height of the base of the caudal fin. Specimens of the Black Sea subspecies had a greater postdorsal length and a larger distance from the end of the dorsal fin base to the end of the anal fin base. A multivariate principal component analysis revealed the highest variability of characters of the body height, which depends on the body condition of the fish and demonstrates seasonal dynamics. The second group combines characters that do not have a seasonal component (dimensions of the head region, lengths of the bases of the dorsal and anal fins, and postdorsal distance) and can be used for distinguishing the shape of the Azov and Black Sea anchovies. The success rate of classification of Azov and Black Sea anchovies according to the body shape measurement characters using discriminant analysis was 83.4%.

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REFERENCES

  1. Alexandrov, A.I., Anchovies of the Azov–Black Sea basin, their origin and taxonomic designation, Tr. Kerch. Nauchn. Rybokhoz. Stn., 1927, vol. 1, nos. 2–3, pp. 37–99.

    Google Scholar 

  2. Altukhov, Yu.P., Limansky, V.V., Payusova, A.N., and Truveler, K.A., Immunogenetic analysis of the intraspecific differentiation in the European anchovy inhabiting the Black and Azov seas. 1. Blood groups of anchovy and a possible mechanism of gene control, Genetika, 1969, vol. 5, no. 4, pp. 50–64.

    Google Scholar 

  3. Afifi, A. and Azen, S., Statistical Analysis: A Computer-Oriented Approach, New York: Academic, 1979.

    Google Scholar 

  4. Vodiasova, E.A. and Abramson, N.I., Genetic variability of anchovy in the Azov–Black Sea basin, Russ. J. Genet., 2017, vol. 53, pp. 680–687. https://doi.org/10.1134/S1022795417060138

    Article  CAS  Google Scholar 

  5. Vodyasova, E.A. and Soldatov, A.A., Identification of subspecies of European anchovy Engraulis encrasicolus (Engraulidae) in the wintering aggregations based on morphological parameters of otoliths, J. Ichthyol., 2017, vol. 57, pp. 553–559. https://doi.org/10.1134/S0032945217040191

    Article  Google Scholar 

  6. Danilevsky, N.N., The penetration of the Black Sea anchovy into the Sea of Azov and concomitant environmental conditions, Tr. Azovo-Chernomorsk. Nauchno-Issled. Inst. Rybn. Khos. Okeanogr., 1960, vol. 18, pp. 118–130.

    Google Scholar 

  7. Dobrovolov, I., Polymorphism of muscle proteins of the anchovy Engraulis encrasicholus (L.) of the Azov-Black Sea basin and the Atlantic Ocean, Vopr. Ikhtiol., 1978, vol. 18, no. 3, pp. 534–540.

    Google Scholar 

  8. Zuyev, G.V., Gutsal, D.K., Melnikova, E.B., and Bondarev, V.A., On the issue of intraspecific heterogeneity of anchovy wintering off the coast of the Crimea, Rybn. Khoz. Ukr., 2007, no. 6, pp. 2–9.

  9. Zuyev, G.V., Gutsal, K.D., Goralevich, K.G., et al., Intraspecific morphological, ecological, and biological variability of Azov–Black Sea European anchovy Engraulis encra-sicolus (Pisces: Engraulidae) wintering near Crimea coastal waters, Morsk. Ekol. Zh., 2011, vol. 10, no. 1, pp. 5–18.

    Google Scholar 

  10. Zuyev, G.V., Bondarev, V.A., Murzin, Yu.L., and Samotoi, Yu.V., Long-term and annual variability of the intraspecific and biological structure of the anchovy Engraulis encrasicolus (L.) (Pisces: Engraulidae) wintering off the eastern coast of the Crimea, Morsk. Ekol. Zh., 2014, vol. 8, no. 2, pp. 31–40.

    Google Scholar 

  11. Kalnin, V.V. and Kalnina, O.V., Genetic differentiation and reproductive relationships of the Azov and Black Sea races of European anchovy. 1. Biochemical polymorphism, Genetika, 1984, vol. 20, no. 2, pp. 303–308.

    Google Scholar 

  12. Kalnina, O.V. and Kalnin, V.V., Genetic differentiation and reproductive relationships of the Azov and Black Sea races of European anchovy. 2. Genetic differences and internal heterogeneity of the Azov and Black Sea races of anchovy, Genetika, 1984, vol. 20, no. 2, pp. 309–313.

    Google Scholar 

  13. Maiorova, A.A., Distribution and fishery of the Black Sea anchovy: A preliminary report, Tr. Azovo-Chernomorsk. Nauchno-Issled. Inst. Rybn. Khoz. Okeanogr., 1950, no. 14, pp. 11–34.

  14. Pavlov, P.I., Fauna Ukrainy (Fauna of Ukraine), vol. 8, part 1: Ryby (Fishes), Kiev: Naukova Dumka, 1980.

  15. Pusanov, I.I. and Tzeeb, Ya.Ya., About anchovy races inhabiting the Black and Azov seas, Tr. Krym. Nauchno-Issled. Inst., 1926, vol. 1, pp. 86–95.

    Google Scholar 

  16. Skazkina, E.P., Differences between Azov anchovy and Black Sea anchovy (Engraulis encrasicolus maeoticus Puzanov, Engraulis encrasicolus ponticus Alex.) by otoliths, Vopr. Ikhtiol., 1965, vol. 5, no. 4, pp. 600–605.

    Google Scholar 

  17. Terekhov, P.A., Contracecosis in the Azov anchovy Engraulis encrasicolus maeoticus Pusanov, Vopr. Ikhtiol., 1979, vol. 19, pp. 759–761.

    Google Scholar 

  18. Chashchin, A.K., Nematode infection of the Black Sea anchovy, Ekspress Inf. TSNIITEIRKh, 1981, no. 3, pp. 7–11.

  19. Shevchenko, N.F., Geographical variability of anchovy in the seas of the Mediterranean basin, Vopr. Ikhtiol., 1980, vol. 20, no. 1, pp. 20–31.

    Google Scholar 

  20. Shlyakhov, V.A., Shlyakhova, O.V., Nadolinsky, V.P., and Perevalov, O.A., Commercial and biological indicators of fisheries for the most important distributed reserves of aquatic biological resources of the Black Sea as the basis for their regional estimation, Vodn. Bioresur. Sreda Obitaniya, 2018, vol. 1, no. 1, pp. 86–103.

    Google Scholar 

  21. Shul’man, G.E., Fiziologo-biokhimicheskie osobennosti godovykh tsiklov ryb (Physiological and Biochemical Characteristics of the Annual Cycles of Fish), Moscow: Pishch. Prom-st., 1972.

  22. Yuneva, T.V., Zabelinsky, S.A., Nikolsky, V.N., et al., Identification of the Azov and Black Sea anchovy in the Black Sea off the coast of Ukraine and Turkey during the fishing period of 2006–2012 based on the content of docosahexaenoic fatty acid in phospholipids, Morsk. Ekol. Zh., 2014, vol. 13, no. 2, pp. 82–89.

    Google Scholar 

  23. Bembo, D.G., Carvalho, G.R., Cingolani, N., et al., Allozymic and morphometric evidence for two stocks of the European anchovy Engraulis encrasicolus in Adriatic waters, Mar. Biol., 1996, vol. 126, pp. 529–538.

    Article  CAS  Google Scholar 

  24. Bonhomme, F., Meyer, L., Arbiol, C., et al., Systematics of European coastal anchovies (genus Engraulis Cuvier), J. Fish Biol., 2022, vol. 100, pp. 594–600.

    Article  PubMed  Google Scholar 

  25. Bookstein, F.L., Morphometric Tools for Landmark Data: Geometry and Biology, New York: Cambridge Univ. Press, 1991.

    Google Scholar 

  26. Cadrin, S.X., Kerr, L.A., and Mariani, S.E., Stock Identification Methods: Applications in Fishery Science, San Diego: Academic, 2014.

    Book  Google Scholar 

  27. Caneco, B., Silva, A., and Morais, A., Morphometric variation among anchovy (Engraulis encrasicholus, L.) populations from the Bay of Biscay and Iberian waters, ICES Counc. Meet., 2004, ICES CM 2004/EE:24.

  28. Chashchin, A., The Black Sea populations of anchovy, Sci. Mar., 1996, vol. 60, no. 2, pp. 219–225.

    Google Scholar 

  29. Düzgüneş, Z.D., Eroğlu, O., Firidin, Ş., and Çakmak, E., Genetic diversity for anchovy populations (Engraulis encrasicolus L.) in the Azov, Marmara and Black Sea, Genet. Aquat. Org., 2018, vol. 2, pp. 53–60.

    Google Scholar 

  30. Elliott, N.G., Haskard, K., and Koslow, J.A., Morphometric analysis of orange roughy (Hoplostethus atlanticus) off the continental slope of southern Australia, J. Fish Biol., 1995, vol. 46, pp. 202–220.

    Article  Google Scholar 

  31. Erdoğan, Z., Turan, C., and Koc, H.T., Morphologic and allozyme analyses of European anchovy (Engraulis encrasicolus (L. 1758)) in the Black, Marmara and Aegean Seas, Acta Adriat., 2009, vol. 50, no. 1, pp. 77–90.

  32. GFCM Stock Assessment Form Small Pelagics—Anchovy 2012–2013; Black Sea Ukrainian Waters, ver. 0.9, GFCM–FAO, 2014.

  33. Guraslan, C., Fach, B.A., and Oguz, T., Understanding the impact of environmental variability on anchovy overwintering migration in Black Sea and its implications for the fishing industry, Front. Mar. Sci., 2017, vol. 4, no. 275, pp. 1–24.

    Article  Google Scholar 

  34. Hammer, Ø., Harper, D.A.T., and Ryan, P.D., PAST: Paleontological statistics software package for education and data analysis, Paleontol. Electron., 2001, vol. 4, no. 1, p. 4.

  35. Ivanova, P.P. and Dobrovolov, I.S., Population-genetic structure on European anchovy (Engraulis encrasicolus, Linnaeus, 1758) (Osteichthyes: Engraulide) from Mediterranean Basin and Atlantic Ocean, Acta Adriat., 2006, vol. 47, no. 1, pp. 13–22.

    Google Scholar 

  36. Kristoffersen, J.B. and Magoulas, A., Population structure of anchovy Engraulis encrasicolus L. in the Mediterranean Sea inferred from multiple methods, Fish. Res., 2008, vol. 91, pp. 187–195.

    Article  Google Scholar 

  37. Rohlf, F.J. and Marcus, L.F., A revolution in morphometrics, Trends Ecol. Evol., 1993, vol. 8, no. 4, pp. 129–132.

    Article  Google Scholar 

  38. Scientific, Technical and Economic Committee for Fisheries (STECF), Black Sea Assessments, Cardinale, M. and Damalas, D., Eds., Luxembourg: Publ. Office Eur. Union, EUR 27517 EN, JRC 98095, 2015.

  39. Strauss, R.E. and Bookstein, F., The truss: Body form reconstructions in morphometrics, Syst. Zool., 1982, vol. 31, pp. 113–135.

    Article  Google Scholar 

  40. Turan, C., Ergüden, D., Gürlek, M., et al., Morphometric structuring of the anchovy (Engraulis encrasicolus L.) in the Black, Aegean and Northeastern Mediterranean Seas, Turk. J. Vet. Anim. Sci., 2004, vol. 28, no. 5, pp. 865–871.

    Google Scholar 

  41. Whitehead, P.J.P., Nelson, G.J., and Wongratana, T., FAO Species Catalogue, vol. 7: Clupeoid Fishes of the World (Suborder Clupeoidei): An Annotated and Illustrated Catalogue of the Herrings, Sardines, Pilchards, Sprats, Shads, Anchovies and Wolf-herrings, Rome: FAO Fish. Synop., 1988, vol. 7, no. 125, pp. 305–579.

    Google Scholar 

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Funding

This research was carried out within the framework of the state assignment (project “Functional, Metabolic and Toxicological Aspects of Aquatic Organisms and Their Populations in Biotopes with Different Physicochemical Regimes”), state registration no. 121041400077-1.

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Correspondence to M. V. Chesalin.

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Translated by T. Koznova

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Chesalin, M.V., Nikolsky, V.N. A Comparative Morphometric Analysis of the Azov Anchovy (Engraulis encrasicolus maeoticus Pusanov, 1926) and the Black Sea Anchovy (E. encrasicolus ponticus Alexandrov, 1927) Based on Body Shape. Russ J Mar Biol 49, 87–96 (2023). https://doi.org/10.1134/S1063074023020049

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