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The Composition and Kinetics of the Hemocyte Population in the Mussel Crenomytilus grayanus (Dunker, 1853)

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Abstract

Based on light microscopy data, hemocytes of Crenomytilus grayanus were classified into five morphological types common for Bivalvia. In the stage of sexual inertia (late October), the proportions of the cell types are as follows: (1) hemoblasts (0.2 ± 0.1%), (2) hyalinocytes (1.9 ± 0.3%), and also (3) basophilic (10.9 ± 1.4%), (4) neutrophilic (13.3 ± 3.0%), and (5) acidophilic (74.1 ± 2.9%) granulocytes. All hemocytes were divided into four groups on the basis of their size (FSC) and complexity (SSC) by flow cytometry. Correlation analysis has shown that R1 corresponds to hemoblasts, R2 to hyalinocytes, and R4 to granulocytes and their acidophilic forms. However, these correlations are not observed in the summer season. The hemocyte morphology and quantitative relationships between their structural types confirm Mix’s hematopoietic model, which postulates histogenetic continuity of hyalinocytes and granulocytes. The arrangement of cells in the light-scatter dot plots (FSC vs. SSC) indicates their maturity stage; it depends on functional status and may change with disturbances of the mitotic cycle. The hemocyte population in C. grayanus shows a low rate of renewal and a dominance of acidophilic granulocytes (up to 99% of all cells in the sexual inertia stage), which suggests a strategy targeted at long-term maintenance of highly differentiated cells and is consistent with the long life expectancy of the species.

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ACKNOWLEDGMENTS

The authors are deeply grateful to V.Ya. Kavun, a senior researcher of the Laboratory of Physiology, Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences (NSCMB FEB RAS), for assistance in collecting the material and to A.V. Boroda, a senior researcher of the Laboratory of Cytotechnology, NSCMB FEB RAS, for organizing the work on a CytoFLEX flow cytometer.

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  1. Cell Biology and Genetics Department, Institute of the World Ocean, Far Eastern Federal University, 690922, Vladivostok, Russia

    A. A. Anisimova, M. N. Diagileva, O. A. Karusheva, A. V. Sinenko & I. A. Dmitrieva

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  1. A. A. Anisimova
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  2. M. N. Diagileva
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Correspondence to A. A. Anisimova.

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Translated by E. Shvetsov

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Anisimova, A.A., Diagileva, M.N., Karusheva, O.A. et al. The Composition and Kinetics of the Hemocyte Population in the Mussel Crenomytilus grayanus (Dunker, 1853). Russ J Mar Biol 48, 256–265 (2022). https://doi.org/10.1134/S1063074022040022

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