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Cytotoxic and Antioxidant Potential of Chloroform Extract of Holothuria tubulosa Gmelin, 1791

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Abstract

Natural products are promising anticancer agents and antioxidants. The present study was aimed to evaluate the cytotoxic and antioxidant potential and the contents of phenolic compounds of a chloroform extract from Holothuria tubulosa collected from the Gökova Gulf (Muğla, Turkey) for the first time. Cell viability was assessed by the MTT assay. Apoptotic cell death was analyzed by flow cytometry. The antioxidant activity of the extract was determined by the DPPH radical scavenging activity assay, the ABTS radical cation scavenging activity assay, the β-carotene-linoleic acid and FRAP assays and by the phosphomolybdenum method. Total flavonoid and tannin contents in the extract were investigated. Also, the phenolic compounds of the extract were detected by HPLC. The extract showed the potential cytotoxic effect against cancer cells and was capable of inducing apoptosis in A549 and HeLa cells. Overall, the antioxidant capacity of the extract may be evaluated as low. The phenolic compound content of the extract was also found to be low. The most abundant component in the extract was ellagic acid (482.293 μg/g extract) according to the HPLC analysis. Our results may contribute to further studies on the use of H. tubulosa for obtaining new anticancer and other therapeutic agents.

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ACKNOWLEDGMENTS

The authors would like to thank the Burdur Mehmet Akif Ersoy University, Scientific and the Technology Application and Research Center, for HPLC analysis and also Özgür Okur from the İzmir Institute of Technology for the help in flow cytometry analysis.

This study is a part of the master’s thesis of the first author accepted at the Department of Molecular Biology and Genetics of Muğla Sıtkı Koçman University in 2020.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    M. Doğangüneş & M. Alper

  2. Department of Aquaculture, Faculty of Fisheries, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    A. Türker

  3. Division of Molecular Biology and Biotechnology, Department of Biology, Faculty of Science, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    H. Güneş

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  1. M. Doğangüneş
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Correspondence to M. Alper.

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Doğangüneş, M., Türker, A., Güneş, H. et al. Cytotoxic and Antioxidant Potential of Chloroform Extract of Holothuria tubulosa Gmelin, 1791. Russ J Mar Biol 47, 498–507 (2021). https://doi.org/10.1134/S1063074021060043

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  • DOI: https://doi.org/10.1134/S1063074021060043

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