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Hydrolytic Enzymes from Marine Organisms as Inhibitors of Biofilm Formation

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Abstract

The effects of some hydrolytic enzymes from marine organisms on the formation and destruction of bacterial biofilms have been studied. As the results show, the presence of α-D-galactosidase from the marine bacterium Pseudoalteromonas sp. KMM 701 stimulates the growth of biofilms formed by various species of marine bacteria, whereas the formation of biofilms by Bacillus subtilis and Yersinia pseudotuberculosis is inhibited by this enzyme. Treatment with α-galactosidase causes destruction of 5 to 35% of a mature biofilm of various bacterial species. Phosphodiesterase and alkaline phosphatase from the marine bacterium Cobetia amphilecti KMM 296 have an inhibitory effect on the biofilm formation by marine strains of Bacillus licheniformis, B. aegricola, and B. berkelogi, and also degrade already formed biofilms of these bacilli and Yersinia. The crab hepatopancreas DNase inhibits the biofilm formation by Y. pseudotuberculosis and B. subtilis by partially degrading a mature biofilm.

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Authors and Affiliations

  1. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    N. A. Terenteva, N. S. Buinovskaya, Yu. A. Noskova, L. V. Slepchenko, O. I. Nedashkovskaya & L. A. Balabanova

  2. School of Biomedicine, Far Eastern Federal University, 690091, Vladivostok, Russia

    L. V. Slepchenko, L. A. Tekuteva & L. A. Balabanova

Authors
  1. N. A. Terenteva
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  2. N. S. Buinovskaya
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  3. Yu. A. Noskova
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  4. L. V. Slepchenko
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  5. O. I. Nedashkovskaya
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  6. L. A. Tekuteva
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  7. L. A. Balabanova
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Correspondence to N. A. Terenteva.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Shvetsov

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Terenteva, N.A., Buinovskaya, N.S., Noskova, Y.A. et al. Hydrolytic Enzymes from Marine Organisms as Inhibitors of Biofilm Formation. Russ J Mar Biol 46, 302–305 (2020). https://doi.org/10.1134/S1063074020040094

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  • DOI: https://doi.org/10.1134/S1063074020040094

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