The High Divergence of Two Morphologically Similar Trematode Species of the Genus Nanophyetus of Salmonids from the Data of mtDNA nad1 Gene Sequences
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Intestinal flukes of fish (mainly salmonids) belonging to the genus Nanophyetus (Trematoda: Troglotrematidae) are the causative agents of nanophyetiasis, a zoonotic disease of animals and humans, which is widespread in countries in the northern Pacific. Two geographical forms, one from North America and the other from the eastern Eurasia were described within this genus; however, their taxonomic status was debatable. A multilocus analysis of nuclear rDNA sequences applied in this study has shown that these forms are independent nominal species: Nanophyetus salmincola and Nanophyetus schikhobalowi. This study, based on sequencing the mtDNA nad1 gene, has evaluated the genetic variability of N. schikhobalowi from eastern Eurasia (Russia) and compared our data with the results obtained for N. salmincola from North America (United States). The genetic differentiation within the Eurasian sample was 1.4%, that for the North American sample was 0.8%, and differentiation between the samples was 15.5%. High values of genetic divergence and completed sorting of mitochondrial haplotypes confirmed the species independence of the compared geographic forms of Nanophyetus.
KeywordsTrematoda Nanophyetus nad1 haplotypes molecular phylogenetics taxonomy
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- 1.Butorina, T.E., Busarova, O.Yu., and Ermolenko, A.V., Parazity gol’tsov Salmonidae: Salvelinus) Golarktiki (Salmonid Parasites (Salmonidae: Salvelinus) of the Holarctic), Vladivostok: Dal’nauka, 2011.Google Scholar
- 2.Dragomeretskaya, A.G., Zelya, O.P., Trotsenko, O.E., and Ivanova, I.B., Social factors of the functioning of nanophyetiasis foci in the Amur region, Med. Parazitol. Parazit. Bolezni, 2014, no. 4, pp. 23–28.Google Scholar
- 3.Ermolenko, A.V., Besprozvannykh, V.V., Rumyantseva, E.E., and Voronok, V.M., Pathogens of trematodoses in humans in Primorskii krai, Med. Parazitol. Parazit. Bolezni, 2015, no. 2, pp. 6–10.Google Scholar
- 4.Sinovich, L.I. and Vostrikov, L.A., Trematody Dal’nego Vostoka, metodicheskie rekomendatsii (Trematodes of the Far East, Methodical Recommendations), Khabarovsk: Khabarovsk. Nauch.–Issled. Inst. Epidemiol. Mikrobiol., 1974, p.4.Google Scholar
- 5.Filimonova, L.V., The spread of nanophyetiasis in the territory of the Soviet Far East, Tr. Gel’mintol. Lab., Akad. Nauk SSSR, 1966, vol. 17, pp. 240–244.Google Scholar
- 9.Chapin, E.A., A new genus and species of trematode, the probable cause of salmon-poisoning in dogs, North Am. Vet., 1926, vol. 7, pp. 36–37.Google Scholar
- 19.Pinto, H.A., Griffin, M.J., Quiniou, S.M., et al., Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: A morphological and molecular study, Parasitol. Res., 2016, vol. 115, no. 1, pp. 51–62.CrossRefPubMedGoogle Scholar
- 23.Skrjabin, K.J. and Podjapolskaja, W.P., Nanophyetus schikhobalowi, n. sp., ein neuer Trematode aus dem Darm des Menschen, Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Abt. Orig., 1931, vol. 119, pp. 294–297.Google Scholar