The Morphological, Genetic and Physiological Characteristics of a Benthic Dinoflagellate of the Genus Ostreopsis Isolated from Coastal Waters of the Northwestern Sea of Japan
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A previous molecular-genetic analysis of clonal cultures and single cells of Ostreopsis spp. that were isolated from Peter the Great Bay (the Sea of Japan) revealed three different genotypes according to the nucleotide sequences of ribosomal DNA (rDNA). Of these, Ostreopsis sp. 1 was earlier found only off the coast of Japan and described as being the most common and highly toxic. We have obtained further molecular evidence and have studied the morphological features of cells from one of the Ostreopsis strains with this genotype and some aspects of its growth at salinities of 24, 28, and 32‰. The results showed that in cells from the Russian coast of the Sea of Japan, the third apical (3') and the third precingular (3") plates do not adjoin one another, being separated by the elongated second apical (2') plate. Cells of Ostreopsis sp. 1 from the Russian coast are significantly larger than Ostreopsis sp. 1 cells with the same genotype from the coast of Japan. Salinity did not have a substantial effect on the growth of Ostreopsis sp. 1. The growth rate varied from 0.24 to 0.35 divisions per day, with maximum values at a salinity of 24‰. When salinity decreased to 24‰, the exponential growth phase was significantly extended and the maximum cell concentration doubled.
KeywordsOstreopsis morphology morphometry growth rate salinity Sea of Japan
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- 1.Konovalova, G.V., Dinoflagellaty (Dinophyta) dal’nevostochnykh morei Rossii i sopredel’nykh akvatorii Tikhogo okeana (Dinoflagellates (Dinophyta) of the Far Eastern Seas of Russia and Adjacent Waters of the Pacific Ocean), Vladivostok: Dalnauka, 1998.Google Scholar
- 13.Efimova, K.V., Orlova, T.Yu., and Brykov, Vl.A., Phylogenetic characterization of cryptic species of the marine dinoflagellate, Ostreopsis sp. Shmidt, 1902, from Russian coastal waters, the Sea of Japan, J. Biodiversity Environ. Sci., 2014, vol. 5, no. 4, pp. 317–332.Google Scholar
- 15.Gallitelli, M., Ungaro, N., Addante, L.M., et al., Respiratory illness as a reaction to tropical algal blooms occurring in a temperate climate, JAMA, 2005, vol. 293, pp. 2595–2600.Google Scholar
- 19.Hoppenrath, M., Murray, S.A., Chomérat, N., and Horiguchi, T., Marine Benthic Dinoflagellates–Unveiling Their Worldwide Biodiversity, Kleine Senckenberg-Reihe, Stuttgart, Germany: Schweizerbart, 2014, vol.54.Google Scholar
- 25.Norris, D.R., Bomber, J.W., and Balech, E., Benthic dinoflagellates associated with ciguatera from the Florida Keys. I. Ostreopsis heptagona sp. nov., Toxic Dinoflagellates, New York: Elsevier, 1985, pp. 39–44.Google Scholar
- 30.Pezzolesi, L., Guerrini, F., Ciminiello, P., et al., Influence of temperature and salinity on Ostreopsis cf. ovata growth and evaluation of toxin content through HR LC-MS and biological assays, Water Resour., 2012, vol. 46, no. 1, pp. 82–92.Google Scholar
- 37.Tanimoto, Y., Yamaguchi, H., Yoshimatsu, T., et al., Effects of temperature, salinity and their interaction on growth of toxic Ostreopsis sp. 1 and Ostreopsis sp. 6 (Dinophyceae) isolated from Japanese coastal waters, Fish. Sci. (Tokyo, Jpn.), 2013, vol. 79, no. 2, pp. 285–291.CrossRefGoogle Scholar
- 39.Tindall, D.R. and Morton, S.L., Community dynamics and physiology of epiphytic/benthic dinoflagellates associated with ciguatera, in Physiological Ecology of Harmful Algal Blooms, NATO ASI Series, Ser. G: Ecological Science, vol. 41, Berlin: Springer-Verlag, 1998, pp. 293–313.Google Scholar
- 42.Verma, A., Hoppenrath, M., Dorantes-Aranda, J.J., et al., Molecular and phylogenetic characterization of Ostreopsis (Dinophyceae) and the description of a new species, Ostreopsis rhodesae sp. nov., from a subtropical Australian lagoon, Harmful Algae, 2016, vol. 60, pp. 116–130.CrossRefPubMedGoogle Scholar