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Russian Journal of Marine Biology

, Volume 44, Issue 1, pp 14–24 | Cite as

The Morphological, Genetic and Physiological Characteristics of a Benthic Dinoflagellate of the Genus Ostreopsis Isolated from Coastal Waters of the Northwestern Sea of Japan

  • M. S. Selina
  • T. V. Morozova
  • N. A. Aizdaicher
  • K. V. Efimova
Article
  • 25 Downloads

Abstract

A previous molecular-genetic analysis of clonal cultures and single cells of Ostreopsis spp. that were isolated from Peter the Great Bay (the Sea of Japan) revealed three different genotypes according to the nucleotide sequences of ribosomal DNA (rDNA). Of these, Ostreopsis sp. 1 was earlier found only off the coast of Japan and described as being the most common and highly toxic. We have obtained further molecular evidence and have studied the morphological features of cells from one of the Ostreopsis strains with this genotype and some aspects of its growth at salinities of 24, 28, and 32‰. The results showed that in cells from the Russian coast of the Sea of Japan, the third apical (3') and the third precingular (3") plates do not adjoin one another, being separated by the elongated second apical (2') plate. Cells of Ostreopsis sp. 1 from the Russian coast are significantly larger than Ostreopsis sp. 1 cells with the same genotype from the coast of Japan. Salinity did not have a substantial effect on the growth of Ostreopsis sp. 1. The growth rate varied from 0.24 to 0.35 divisions per day, with maximum values at a salinity of 24‰. When salinity decreased to 24‰, the exponential growth phase was significantly extended and the maximum cell concentration doubled.

Keywords

Ostreopsis morphology morphometry growth rate salinity Sea of Japan 

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References

  1. 1.
    Konovalova, G.V., Dinoflagellaty (Dinophyta) dal’nevostochnykh morei Rossii i sopredel’nykh akvatorii Tikhogo okeana (Dinoflagellates (Dinophyta) of the Far Eastern Seas of Russia and Adjacent Waters of the Pacific Ocean), Vladivostok: Dalnauka, 1998.Google Scholar
  2. 2.
    Selina, M.S. and Levchenko, E.V., Species composition and morphology of dinoflagellates (Dinophyta) of epiphytic assemblages of Peter the Great Bay in the Sea of Japan, Russ. J. Mar. Biol., 2011, vol. 37, no. 1, pp. 23–32.CrossRefGoogle Scholar
  3. 3.
    Accoroni, S., Romagnoli, T., Penna, A., et al., Ostreopsis fattorussoi sp. nov. (Dinophyceae), a new benthic toxic Ostreopsis species from the eastern Mediterranean Sea, J. Phycol., 2016, vol. 52, no. 6, pp. 1064–1084.CrossRefPubMedGoogle Scholar
  4. 4.
    Accoroni, S., Romagnoli, T., Pichierri, S., et al., Morphometric analysis of Ostreopsis cf. ovata cells in relation to environmental conditions and bloom phases, Harmful Algae, 2012, vol. 19, pp. 15–22.CrossRefGoogle Scholar
  5. 5.
    Adachi, M., Sako, Y., and Ishida, Y., Restriction fragment length polymorphism of ribosomal DNA internal transcribed spacer and 5.8S regions in Japanese Alexandrium species (Dinophyceae), J. Phycol., 1994, vol. 30, no. 5, pp. 857–863.CrossRefGoogle Scholar
  6. 6.
    Aligizaki, K. and Nikolaidis, G., The presence of the potentially toxic genera Ostreopsis and Coolia (Dinophyceae) in the North Aegean Sea, Greece, Harmful Algae, 2006, vol. 5, pp. 717–730.CrossRefGoogle Scholar
  7. 7.
    Bravo, I., Vila, M., Casabianca, S., et al., Life cycle stages of the benthic palytoxin-producing dinoflagellate Ostreopsis cf. ovata (Dinophyceae), Harmful Algae, 2012, vol. 18, pp. 24–34.CrossRefGoogle Scholar
  8. 8.
    Carnicer, O., García-Altares, M., Andree, K.B., et al., Ostreopsis cf. ovata from western Mediterranean Sea: physiological responses under different temperature and salinity conditions, Harmful Algae, 2016, vol. 57, pp. 98–108.CrossRefGoogle Scholar
  9. 9.
    Chinain, M., Faust, M.A., and Paullac, S., Morphology and molecular analyses of three toxic species of Gambierdiscus (Dinophyceae): G. pacificus, sp. nov., G. australes, sp. nov., and G. polynesiensis, sp. nov., J. Phycol., 1999, vol. 35, no. 6, pp. 1282–1296.CrossRefGoogle Scholar
  10. 10.
    Ciminiello, P., Dell’Aversano, C., Fattorusso, E., et al., Putative palytoxin and its new analogue, ovatoxin-a, in Ostreopsis ovata collected along the Ligurian coasts during the 2006 toxic outbreak, J. Am. Soc. Mass Spectrom., 2008, vol. 19, no. 1, pp. 111–120.CrossRefPubMedGoogle Scholar
  11. 11.
    Darriba, D., Taboada, G.L., Doallo, R., et al., jModelTest 2: more models, new heuristics and parallel computing, Nat. Methods, 2012, vol. 9, no. 8, pp. 772–772.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    David, H., Laza-Martínez, A., Miguel, I., and Orive, E., Ostreopsis cf. siamensis and Ostreopsis cf. ovata from the Atlantic Iberian Peninsula: morphological and phylogenetic characterization, Harmful Algae, 2013, vol. 30, pp. 44–55.CrossRefGoogle Scholar
  13. 13.
    Efimova, K.V., Orlova, T.Yu., and Brykov, Vl.A., Phylogenetic characterization of cryptic species of the marine dinoflagellate, Ostreopsis sp. Shmidt, 1902, from Russian coastal waters, the Sea of Japan, J. Biodiversity Environ. Sci., 2014, vol. 5, no. 4, pp. 317–332.Google Scholar
  14. 14.
    Faimali, M., Giussani, V., Piazza V., et al., Toxic effects of harmful benthic dinoflagellate Ostreopsis ovata on invertebrate and vertebrate marine organisms, Mar. Environ. Res., 2012, vol. 76, pp. 97–107.CrossRefPubMedGoogle Scholar
  15. 15.
    Gallitelli, M., Ungaro, N., Addante, L.M., et al., Respiratory illness as a reaction to tropical algal blooms occurring in a temperate climate, JAMA, 2005, vol. 293, pp. 2595–2600.Google Scholar
  16. 16.
    Giussani, V., Costa, E., Pecorino, D., et al., Effects of the harmful dinoflagellate Ostreopsis cf. ovata on different life cycle stages of the common moon jellyfish Aurelia sp., Harmful Algae, 2016, vol. 57, pp. 49–58.CrossRefGoogle Scholar
  17. 17.
    Granáli, E., Vidyarathna, N.K., Funari, E., et al., Can increases in temperature stimulate blooms of the toxic benthic dinoflagellate Ostreopsis ovata?, Harmful Algae, 2011, vol. 10, no. 2, pp. 165–172.CrossRefGoogle Scholar
  18. 18.
    Guindon, S., Dufayard, J.F., Lefort, V., et al., New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0, Syst. Biol., 2010, vol. 59, no. 3, pp. 307–321.CrossRefPubMedGoogle Scholar
  19. 19.
    Hoppenrath, M., Murray, S.A., Chomérat, N., and Horiguchi, T., Marine Benthic Dinoflagellates–Unveiling Their Worldwide Biodiversity, Kleine Senckenberg-Reihe, Stuttgart, Germany: Schweizerbart, 2014, vol.54.Google Scholar
  20. 20.
    Kameneva, P.A., Efimova, K.V., Rybin, V.G., and Orlova, T.Yu., Detection of dinophysistoxin-1 in clonal culture of marine dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan, Toxins, 2015, vol. 7, no. 10, pp. 3947–3959.CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Mangialajo, L., Ganzin, N., Accoroni, S., et al., Trends in Ostreopsis proliferation along the Northern Mediterranean coasts, Toxicon, 2011, vol. 57, no. 3, pp. 408–420.CrossRefPubMedGoogle Scholar
  22. 22.
    Monti, M., Minocci, M., Beran A., and Iveša, L., First record of Ostreopsis cf. ovata on macroalgae in the Northern Adriatic Sea, Mar. Pollut. Bull., 2007, vol. 54, no. 5, pp. 598–601.CrossRefPubMedGoogle Scholar
  23. 23.
    Morton, S.L., Norris, D.R., and Bomber, J.W., Effect of temperature, salinity and light intensity on the growth and seasonality of toxic dinoflagellates associated with ciguatera, J. Exp. Mar. Biol. Ecol., 1992, vol. 157, no. 1, pp. 79–90.CrossRefGoogle Scholar
  24. 24.
    Nascimento, S.M., Corrêa, E.V., Menezes, M., et al., Growth and toxin profile of Ostreopsis cf. ovata (Dinophyta) from Rio de Janeiro, Brazil, Harmful Algae, 2012, vol. 13, pp. 1–9.CrossRefGoogle Scholar
  25. 25.
    Norris, D.R., Bomber, J.W., and Balech, E., Benthic dinoflagellates associated with ciguatera from the Florida Keys. I. Ostreopsis heptagona sp. nov., Toxic Dinoflagellates, New York: Elsevier, 1985, pp. 39–44.Google Scholar
  26. 26.
    Parsons, M.L., Aligizaki, K., Bottein, M.-Y.D., et al., Gambierdiscus and Ostreopsis: Reassessment of the state of knowledge of their taxonomy, geography, ecophysiology, and toxicology, Harmful Algae, 2012, vol. 14, pp. 107–129.CrossRefGoogle Scholar
  27. 27.
    Penna, A., Bertozzini, E., Battocchi, C., et al., Monitoring of HAB species in the Mediterranean Sea through molecular methods, J. Plankton Res., 2007, vol. 29, no. 1, pp. 19–38.CrossRefGoogle Scholar
  28. 28.
    Penna, A., Fraga, S., Battocchi, C., et al., A phylogeographical study of the toxic benthic dinoflagellate genus Ostreopsis Schmidt, J. Biogeogr., 2010, vol. 37, no. 5, pp. 830–841.CrossRefGoogle Scholar
  29. 29.
    Penna, A., Vila, M., Fraga, S., et al., Characterization of Ostreopsis and Coolia (Dinophyceae) isolates in the western Mediterranean Sea based on morphology, toxicity and internal transcribed spacer 5.8S rDNA sequences, J. Phycol., 2005, vol. 41, no. 1, pp. 212–225.CrossRefGoogle Scholar
  30. 30.
    Pezzolesi, L., Guerrini, F., Ciminiello, P., et al., Influence of temperature and salinity on Ostreopsis cf. ovata growth and evaluation of toxin content through HR LC-MS and biological assays, Water Resour., 2012, vol. 46, no. 1, pp. 82–92.Google Scholar
  31. 31.
    Pistocchi, R., Pezzolesi, L., Guerrini, F., et al., A review on the effects of environmental conditions on growth and toxin production of Ostreopsis ovata, Toxicon, 2011, vol. 57, no. 3, pp. 421–428.CrossRefPubMedGoogle Scholar
  32. 32.
    Rhodes, L., World-wide occurrence of the toxic dinoflagellate genus Ostreopsis Schmidt, Toxicon, 2011, vol. 57, no. 3, pp. 400–407.CrossRefPubMedGoogle Scholar
  33. 33.
    Sato, S., Nishimura, T., Uehara, K., et al., Phylogeography of Ostreopsis along west Pacific coast, with special reference to a novel clade from Japan, PLoS One, 2011, vol. 6, no. 12, p. e27983.CrossRefPubMedPubMedCentralGoogle Scholar
  34. 34.
    Selina, M.S., Morozova,T.V., Vyshkvartsev, D.I., and Orlova, T.Yu., Seasonal dynamics and spatial distribution of epiphytic dinoflagellates in Peter the Great Bay (Sea of Japan) with special emphasis on Ostreopsis species, Harmful Algae, 2014, vol. 32, pp. 1–10.CrossRefGoogle Scholar
  35. 35.
    Selina, M.S. and Orlova, T.Yu., First occurrence of the genus Ostreopsis (Dinophyceae) in the Sea of Japan, Bot. Mar., 2010, vol. 53, pp. 243–249.CrossRefGoogle Scholar
  36. 36.
    Suzuki, T., Watanabe, R., Uchida, H., et al., LC-MS/MS analysis of novel ovatoxin isomers in several Ostreopsis strains collected in Japan, Harmful Algae, 2012, vol. 20, pp. 81–91.CrossRefGoogle Scholar
  37. 37.
    Tanimoto, Y., Yamaguchi, H., Yoshimatsu, T., et al., Effects of temperature, salinity and their interaction on growth of toxic Ostreopsis sp. 1 and Ostreopsis sp. 6 (Dinophyceae) isolated from Japanese coastal waters, Fish. Sci. (Tokyo, Jpn.), 2013, vol. 79, no. 2, pp. 285–291.CrossRefGoogle Scholar
  38. 38.
    Tawong, W., Nishimura, T., Sakanari, H., et al., Distribution and molecular phylogeny of the dinoflagellate genus Ostreopsis in Thailand, Harmful Algae, 2014, vol. 37, pp. 160–171.CrossRefGoogle Scholar
  39. 39.
    Tindall, D.R. and Morton, S.L., Community dynamics and physiology of epiphytic/benthic dinoflagellates associated with ciguatera, in Physiological Ecology of Harmful Algal Blooms, NATO ASI Series, Ser. G: Ecological Science, vol. 41, Berlin: Springer-Verlag, 1998, pp. 293–313.Google Scholar
  40. 40.
    Totti, C., Accoroni, S., Cerino F., et al., Ostreopsis ovata bloom along the Conero Riviera (northern Adriatic Sea): relationships with environmental conditions and substrata, Harmful Algae, 2010, vol. 9, no. 2, pp. 233–239.CrossRefGoogle Scholar
  41. 41.
    Tubaro, A., Durando, P., Del Favero, G., et al., Case definitions for human poisonings postulated to palytoxins exposure, Toxicon, 2011, vol. 57, no. 3, pp. 478–495.CrossRefPubMedGoogle Scholar
  42. 42.
    Verma, A., Hoppenrath, M., Dorantes-Aranda, J.J., et al., Molecular and phylogenetic characterization of Ostreopsis (Dinophyceae) and the description of a new species, Ostreopsis rhodesae sp. nov., from a subtropical Australian lagoon, Harmful Algae, 2016, vol. 60, pp. 116–130.CrossRefPubMedGoogle Scholar
  43. 43.
    Yamaguchi, H., Yoshimatsu, T., Tanimoto, Y., et al., Effects of temperature, salinity and their interaction on growth of the benthic dinoflagellate Ostreopsis cf. ovata (Dinophyceae) from Japanese coastal waters, Phycol. Res., 2012, vol. 60, no. 4, pp. 297–304.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • M. S. Selina
    • 1
  • T. V. Morozova
    • 1
  • N. A. Aizdaicher
    • 1
  • K. V. Efimova
    • 1
    • 2
  1. 1.Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern BranchRussian Academy of SciencesVladivostokRussia
  2. 2.Far Eastern Federal UniversityVladivostokRussia

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