The dynamics of heat production in erythrocytes of the scorpion fish (Scorpaena porcus Linnaeus, 1758) in vitro
- 20 Downloads
Temperature measurements in a plastic tube isolated from external influences containing an erythrocyte suspension of the scorpion fish (Scorpaena porcus Linnaeus, 1758) showed that these red blood cells are able to generate heat. Heat release in the cell suspension was expressed by a linear temperature increase in the tube during the entire experiment. Addition of extracellular ATP (1 mg mL–1) caused the effect of a thermal shift: a sharp temperature rise in the cell suspension for 30–60 s. We believe that the heat release was caused by hydrolysis of extracellular ATP by membrane ecto-ATPase. Inhibition of ecto-ATPase activity through the addition of EDTA (1 mM) to the erythrocyte suspension led to complete blockage of heat release; the effect of the thermal shift ceased. We assume that thermal properties of red blood cells play an important role in blood hemodynamics, especially in providing the “non-Newtonian” properties of blood. The thermal phenomena observed in suspensions of fish erythrocytes open new scientific directions in exploring the capabilities of multifunctional extracellular ATP.
Keywordstemperature heat production red blood cells ATP membrane ecto-ATPase fish
Unable to display preview. Download preview PDF.
- 2.Venkstern, T.V. and Engelhardt, V.A., Distribution of ecto-adenosinpolyphosphatase and characterization of some of its properties, Biochemistry, 1957, vol. 22, no. 5, pp. 911–916.Google Scholar
- 3.Evans, E.A. and Skalak, R., Mechanics and Thermodynamics of Biomembranes, Boca Raton, FL: CRC, 1980.Google Scholar
- 5.Murav’ev, A.V., Tikhomirova, I.A., Maimistova, A.A., et al., The role of the microrheological properties of erythrocytes in non-Newtonian behavior of whole blood, Ross. Zh. Biomekhaniki, 2010, vol. 14, no. 4, pp. 96–104.Google Scholar
- 6.Silkin, Yu.A. and Silkina, E.N., Mg-Dependent Ecto-ATPase of the erythrocyte plasma membrane of the scorpion fish Scorpaena porcus: Biochemical properties and kinetic characteristics, J. Evol. Biochem. Phyiol., 2000, vol. 36, no. 5, pp. 519–524.Google Scholar
- 8.Soldatov, A.A., Parfenova, I.A., and Nowicka, V.N., The content of monovalent cations and ATP in erythrocytes of sea fish in experimental hypoxia, Ukr. Biokhim. Zh., 2010, vol. 82, no. 2, pp. 36–41.Google Scholar
- 14.Extracellular ATP and Adenosine as Regulators of Endothelial Cell Function, Gerasimovskaya, E. and Kaczmarek, E., Eds., New York: Springer-Verlag, 2010.Google Scholar
- 15.Fâhraeus, R. and Lindqvist, T., The viscosity of the blood in narrow capillary tubes, Am. J. Physiol., 1931, vol. 96, pp. 562–568.Google Scholar
- 17.Katiukhin, L.N., About a mechanism of the Fâhraeus-Lindqvist effect, J. Blood Disord. Transfus., 2014, vol. 5, no. 5, pp. 211–213.Google Scholar
- 21.Philips, M.C., Moyes, C.D., and Tufts, B.L., The effects of cell aging on metabolism in rainbow trout (Oncorhynchus mykiss) red blood cells, J. Exp. Biol., 2000, vol. 203, pp. 1039–1045.Google Scholar
- 23.Stolbov, A.Y., Mishurov, V.G., and Shadrin, N.V., The macrocalorimetric method in hydrobiology: Description of the pilot device, Ekol. Morya, 2009, vol. 77, pp. 94–96.Google Scholar