The composition and seasonal dynamics of the hemocyte cell population in the clams Corbicula japonica Prime (1864) of the Kievka River (the basin of the Sea of Japan)
- 32 Downloads
The parameters of a hemocyte cell population were investigated in the bivalve mollusk Corbicula japonica of the Kievka River (the basin of the Sea of Japan) at four seasonal points corresponding to different stages of the reproductive cycle: (1) March (sexual rest); (2) May (gametogenesis); (3) July (spawning); and (4) November (the end of reproductive activity). Using light microscopy, we identified four cell morphotypes in the hemolymph: small and large hyalinocytes, as well as basophilic and eosinophilic granulocytes. Flow cytometry confirmed the structural heterogeneity of the cell population and allowed us to estimate the seasonal variability of the cell composition in C. japonica hemolymph. The total cell count in the mollusk hemolymph did not change significantly during the annual cycle and did not correlate with the average cell size of hemocytes. In March, May, and November, granulocytes dominated in the cell population (74.3 ± 2.9, 77.5 ± 3.5, and 86.7 ± 2.6%, respectively), while in July their relative content was reduced dramatically (37.2 ± 5.1%) causing a significant decrease of both the average cell size and granularity in circulation. Most likely, this is connected with the summer migration of mature eosinophilic granulocytes from the hemolymph to spawning gonads for resorption of the unspent sexual products, as well as to other internal organs for participation in digestion and to provide the immune defense against pathogens.
Keywordshemocytes hemolymph seasonal dynamics reproductive cycle Corbicula japonica Bivalvia
Unable to display preview. Download preview PDF.
- 2.Volova, G.N., Macrobenthos of bracking-water bodies of southern Primorye (Sea of Japan), Gidrobiol. Zh., 1974, vol. 10, no. 6, pp. 32–37.Google Scholar
- 3.Dzyuba, S.M. and Maslennikova, L.A., Reproductive cycle of the bivalve mollusks Anadara broughtoni from the southern part of Peter the Great Bay (Sea of Japan), Biol. Morya (Vladivostok), 1982, no. 3, pp. 34–40.Google Scholar
- 4.Zavarzin, A.A., Comparative histology of the blood and connective tissue: IV. Inflammatory of connective tissue neoplasms in the mussel (Anodonta anatine L.), in Izbrannye trudy (Selected Research Works), Moscow: Akad. Nauk SSSR, 1953, vol. 2.Google Scholar
- 6.Yavnov, S.V. and Rakov, V.A., Korbikula (Corbicula Species), Vladivostok: TINRO-Center, 2002.Google Scholar
- 12.Chang, S.-J., Tseng, S.-M., and Chou, H.-Y., Morphological characterization via light and electron microscopy of the hemocytes of two cultured bivalves: A comparison study between the hard clam (Meretrix lusoria) and Pacific oyster (Crassostrea gigas), Zool. Stud., 2005, vol. 44, no. 1, pp. 144–153.Google Scholar
- 13.Cheng, T.C., Bivalves, in Invertebrate Blood Cells, London: Academic, 1981, vol. 2, pp. 233–300.Google Scholar
- 15.Chu, F.L.E., Volety, A.K., and La Peyre, J.F., Annual variation of hemolymph components and Perkinsus marinus infection in oysters sampled from deep water shoal, James River, Virginia, J. Shellfish Res., 1995, vol. 14, p. 263.Google Scholar
- 19.Fisher, W.S., Oliver, L.M., and Edwards, P., Hematologic and serologic variability of eastern oysters from Apalachicola Bay, Florida, J. Shellfish Res., 1996, vol. 15, pp. 555–564.Google Scholar
- 28.Mix, M.C., A general model for leucocyte cell renewal in bivalve mollusks, Mar. Fish. Rev., 1976, vol. 38, no. 10, pp. 37–41.Google Scholar
- 35.Renwrantz, L., Siegmund, E., and Woldmann, M., Variations in hemocyte counts in the mussel, Mytilus edulis: Similar reaction patterns occur in disappearance and return of molluscan hemocytes and vertebrate leukocytes, Comp. Biochem. Physiol., Part A: Mol. Integr. Physiol., 2013, vol. 164, pp. 629–637.CrossRefGoogle Scholar
- 38.Volety, A.K., Winstead, J.T., and Fisher, W.S., Influence of seasonal factors on oyster hemocyte killing of Vibrio parahaemolyticus, J. Shellfish Res., 1999, vol. 18, p. 323.Google Scholar