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The functional activity of fractions of coelomocytes of the starfish Asterias rubens Linnaeus, 1758

  • Evolutionary Physiology
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Abstract

As a result of the centrifugation of the circulating cells of the starfish Asterias rubens in a discontinuous density gradient of sodium diatrizoate, three cell fractions were separated. Small coelomocytes with a high nuclear–cytoplasmic ratio and the lack of granules prevailed in the upper fraction, coelomocytes with small granules evenly distributed in their cytoplasm dominated in the middle fraction, and large coelomocytes with a high content of large granules and vesicles in the perinuclear space were predominant in the bottom fraction. The coelomocytes of the separated fractions were tested for the production of reactive oxygen species, neutral red uptake, capture and internalization of the labeled bacteria Escherichia coli and Staphylococcus aureus, as well as for lytic activity and expression of the ArC3-like gene, which is a homologue of the C3 component of the mammalian complement cascade. Cells of the upper fraction manifested the most pronounced ability for the expression of the C3 gene homologue in response to stimulation with bacterial lipopolysaccharide. Coelomocytes of the middle fraction were distinguished by a pronounced ability to produce reactive oxygen species and phagocytosis, whereas the cells of the lower fraction had a high level of hemolytic activity and neutral red uptake.

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References

  1. Gorshkov, A.N., Blinova, M.I., and Pinaev, G.P., Ultrastructure of coelomic epithelium and coelomocytes of the starfish Asterias rubens L. in norm and after wounding, Cell Tissue Biol., 2009, vol. 3, pp. 477–490.

    Article  Google Scholar 

  2. Kozlova, A.B., Petukhova, O.A., and Pinaev, G.P., Analysis of the cellular elements of coelomic fluid in the early stages of regeneration of the starfish Asterias rubens L., Tsitologiya, 2006, vol. 48, pp. 175–183.

    CAS  Google Scholar 

  3. Korenbaum, E.S., Ultrastructure of coelomocytes of the starfish Asterias amurensis, Tsitologiya, 1989, vol. 31, pp. 1165–1171.

    Google Scholar 

  4. Kudryavtsev, I.V., D’yachkov, I.S., Kazakov, A.A., et al., Humoral responses of congenital immunity in the starfish Asterias rubens, J. Evol. Biochem. Physiol., 2005, vol. 41, no. 1, pp. 26–33.

    Article  CAS  Google Scholar 

  5. Kudryavtsev, I.V., D’yachkov, I.S., Kazakov, A.A., et al., Cellular responses of congenital immunity in the starfish Asterias rubens, J. Evol. Biochem. Physiol., 2005, vol. 41, no. 2, pp. 134–142.

    Article  Google Scholar 

  6. Kudryavtsev, I.V. and Polevshchikov, A.V., Comparative-immunological analysis of the cellular and humoral protective factors in echinoderms, Zh. Obsh. Biol., 2004, vol. 65, pp. 218–231.

    Google Scholar 

  7. Mogilenko, D.A., Kudryavtsev, I.V., Orlov, S.V., et al., Expression of the starfish complement component C3 gene homolog under the influence of bacterial lipopolysaccharide, Mol. Biol. (Moscow), 2010, vol. 44, no.1, pp. 67–76.

    Article  CAS  Google Scholar 

  8. Brockton, V., Henson, J.H., Raftos, D.A., et al., Localization and diversity of 185/333 proteins from the purple sea urchin–unexpected protein-size range and protein expression in a new coelomocyte type, J. Cell Sci., 2008, vol. 121, pp. 339–348.

    Article  CAS  PubMed  Google Scholar 

  9. Canicatti, C., Lysosomal enzyme pattern in Holothuria polii coelomocytes, J. Invertebr. Pathol., 1990, vol. 56, pp. 70–74.

    Article  CAS  Google Scholar 

  10. Coteur, G., Danis, B., Fowler, S.W., et al., Effects of PCB’s on reactive oxygen species (ROS) production by the immune cells of Paracentrotus lividus (Echinodermata), Mar. Pollut. Bull., 2001, vol. 42, pp. 667–672.

    Article  CAS  PubMed  Google Scholar 

  11. Coteur, G., DeBecker, G., Warnau, M., et al., Differentiation of immune cells challenged by bacteria in the common European starfish, Asterias rubens (Echinodermata), Eur. J. Cell Biol., 2002, vol. 81, pp. 413–418.

    Article  PubMed  Google Scholar 

  12. Edds, K.T., Cell biology of echinoid coelomocytes. I. Diversity and characterization of cell types, J. Invertebrate Pathol., 1993, vol. 61, pp. 173–178.

    Article  Google Scholar 

  13. Fontain, A.R. and Lambert, P., The fine structure of the leukocytes of the holothurian, Cucumaria miniata, Can. J. Zool., 1977, vol. 55, pp. 1530–1544.

    Article  Google Scholar 

  14. Gross, P.S., Al-Sharif, W.Z., Clow, L.A., et al., Echinoderm immunity and the evolution of the complement system, Dev. Comp. Immunol., 1999, vol. 23, pp. 429–442.

    Article  CAS  PubMed  Google Scholar 

  15. Gross, P.S., Clow, L.A., and Smith, L.C., SpC3, the complement homologue from the purple sea urchin, Strongylocentrotus purpuratus, is expressed in two subpopulations of the phagocytic coelomocytes, Immunogenetics, 2000, vol. 51, pp. 1034–1044.

    Article  CAS  PubMed  Google Scholar 

  16. Hauton, Ch. and Smith, V.J., In vitro cytotoxicity of crustacean immunostimulants for lobster (Homarus gammarus) granulocytes demonstrated using the neutral red uptake assay, Fish Shellfish Immunol., 2004, vol. 17, pp. 65–73.

    Article  CAS  PubMed  Google Scholar 

  17. Jangoux, M. and Vanden-Bossche, J.-P., Morphology and dynamics of the coelomocytes of Asterias rubens L. (Echinodermata, Asteroidea), Forma Funct., 1975, vol. 8, pp. 191–208.

    Google Scholar 

  18. Matranga, V., Toia, G., Bonaventura, R., et al., Cellular and biochemical responses to environmental and experimentally induced stress in sea urchin coelomocytes, Cell Stress Chaperones, 2000, vol. 5, pp. 113–120.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Messer, L.I. and Wardlaw, A.C., Separation of the coelomocytes of Echinus esculentus by density gradient centrifugation, Echinoderms: Present and Past. Proc. Eur. Colloquium on Echinoderms, Rotterdam: Balkema, 1979), pp. 319–323.

    Google Scholar 

  20. Nair, S.V., Del Valle, H., Gross P.S., et al., Macroarray analysis of coelomocyte gene expression in response to LPS in the sea urchin. Identification of unexpected immune diversity in an invertebrate, Physiol. Genomics, 2005, vol. 22, pp. 33–47.

    Article  CAS  PubMed  Google Scholar 

  21. Pagliara, P. and Canicatti, C., Isolation of cytolytic granules from sea urchin amoebocytes, Eur. J. Cell Biol., 1993, vol. 60, pp. 179–184.

    CAS  PubMed  Google Scholar 

  22. Ramírez-Gomez, F., Aponte-Rivera, F., Mendez-Castaner, L., and García-Arraras, J.E., Changes in holothurian coelomocyte populations following immune stimulation with different molecular patterns, Fish Shellfish Immunol., 2010, vol. 29, pp. 175–185.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Santiago-Cardona, P.G., Berrios, C.A., Ramirez, F., et al., Lipopolysaccharides induce intestinal serum amyloid A expression in the sea cucumber Holothuria glaberrima, Dev. Comp. Immunol., 2003, vol. 27, pp. 105–110.

    Article  CAS  PubMed  Google Scholar 

  24. Smith, L.C., Britten, R.J., and Davidson, E.H., SpCoel1, a sea urchin profilin gene expressed specifically in coelomocytes in response to injury, Mol. Biol. Cell, 1992, vol. 3, no. 4, pp. 403–414.

    CAS  Google Scholar 

  25. Smith L.C., Chang L., Britten R.J., et al., Sea urchin genes expressed in activated coelomocytes are identified by expressed sequence tags. Complement homologues and other putative immune response genes suggest immune system homology within the deuterostomes, J. Immunol., 1996, vol. 156, pp. 593–602.

    CAS  PubMed  Google Scholar 

  26. Vethamany, V.G. and Fung, M., The fine structure of the coelomocytes of the sea urchin Strongylocentrotus droebachiensis, Can. J. Zool., 1972, vol. 50, pp. 77–81.

    Article  Google Scholar 

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Correspondence to I. V. Kudryavtsev.

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Original Russian Text © I.V. Kudryavtsev, I.S. D’yachkov, D.A. Mogilenko, A.N. Sukhachev, A.V. Polevshchikov, 2016, published in Biologiya Morya.

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Kudryavtsev, I.V., D’yachkov, I.S., Mogilenko, D.A. et al. The functional activity of fractions of coelomocytes of the starfish Asterias rubens Linnaeus, 1758. Russ J Mar Biol 42, 158–165 (2016). https://doi.org/10.1134/S106307401602005X

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  • DOI: https://doi.org/10.1134/S106307401602005X

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