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Hemolymph cholinesterase activity in the Mussel Crenomytilus grayanus (Dunker, 1853) that was exposed to adverse natural and anthropogenic conditions

  • Biochemical Adaptation
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Abstract

The influence of habitat conditions on the activity, the structure of the substrate specificity (the ratio of the substrate hydrolysis rates), and the kinetic parameters of substrate hydrolysis due to the effect of hemolymph cholinesterase of the mussel Crenomytilus grayanus was studied. Mussels were collected from areas that are influenced by seasonal and stationary upwelling, as well as from a polluted area. Upwelling and anthropogenic pressure were shown to alter the structure of hemolymph cholinesterase substrate specificity in mussels, up to complete loss of the ability to catalyze the hydrolysis of propionyland butyrylthiocholine. It was established that during the seasonal upwelling the efficiency of the cholinergic process in mussels is provided by a wide range of effective concentrations of the substrates and by decreasing their affinity to the enzyme. Under the conditions of chronic anthropogenic pollution, the cholinesterase of the mussel hemolymph loses its ability to hydrolyze substrates other than acetylthiocholine.

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Authors and Affiliations

  1. Far Eastern Federal University, ul. Sukhanova 8, Vladivostok, 690950, Russia

    N. N. Kovalev, E. Ya. Kostetsky & O. V. Podgurskaya

  2. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, ul. Pal’chevskogo 17, Vladivostok, 690041, Russia

    V. Ya. Kavun & O. V. Podgurskaya

  3. Far Eastern State Technical Fisheries University, ul. Lugovaya 52b, Vladivostok, 690087, Russia

    N. N. Kovalev & Ye. V. Mikheev

Authors
  1. N. N. Kovalev
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  2. V. Ya. Kavun
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  3. E. Ya. Kostetsky
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  4. Ye. V. Mikheev
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  5. O. V. Podgurskaya
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Correspondence to V. Ya. Kavun.

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Original Russian Text © N.N. Kovalev, V.Ya. Kavun, E.Ya. Kostetsky, Ye.V. Mikheev, O.V. Podgurskaya, 2016, published in Biologiya Morya.

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Kovalev, N.N., Kavun, V.Y., Kostetsky, E.Y. et al. Hemolymph cholinesterase activity in the Mussel Crenomytilus grayanus (Dunker, 1853) that was exposed to adverse natural and anthropogenic conditions. Russ J Mar Biol 42, 65–72 (2016). https://doi.org/10.1134/S1063074016010120

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  • DOI: https://doi.org/10.1134/S1063074016010120

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