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Isolation and partial characterization of bacteria with potential antimicrobial activity from the Caspian Sea

  • Microbiology
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Abstract

Due to the constant increasing of bacterial resistance against known antibiotics, it is now necessary to find new sources of antimicrobials including the marine environment. The aim of this study was to evaluate antimicrobial activity of bacterial strains isolated from different coastal regions of the Caspian Sea and to provide phylogenetic analyses of antibiotic producing strains. Water samples collected from the Caspian Sea were serially diluted and plated on selective media. Isolates were tested against a panel of reference strains (Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa) by microbial antagonism and disc diffusion assay. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method was also employed to produce a phylogenetic tree based on 16S rDNA sequences. Amongst 162 isolates, 8 strains (4.93%) showed antibacterial activity. Isolated bacteria displayed more activity against gram-positive bacteria than gram-negative bacteria. Moreover, the 16S sequences obtained for the 8 selected strains were compared using a BLAST algorithm and allowed us to determine the strains genus as followed: Bacillus (RS28, RS54, RS56, RS82, RS116, and NS53), Brevundimonas (RS32), and Arthrobacter (NS25). The findings of the present study recommend that culturally marine bacteria collected from the Caspian Sea might be a potent source of novel bioactive compounds such as antibiotics.

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Authors and Affiliations

  1. Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

    Sajad Harounabadi & Seyed Mansour Meybodi

  2. Department of Environment and Energy, Science Research Branch, Islamic Azad University, Tehran, Iran

    Parvaneh Eghbali Shamsabad

  3. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Seyyed Khalil Shokouhi Mostafavi

Authors
  1. Sajad Harounabadi
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  2. Parvaneh Eghbali Shamsabad
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  3. Seyyed Khalil Shokouhi Mostafavi
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  4. Seyed Mansour Meybodi
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Correspondence to Sajad Harounabadi.

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The text was submitted by the authors in English.

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Harounabadi, S., Shamsabad, P.E., Mostafavi, S.K.S. et al. Isolation and partial characterization of bacteria with potential antimicrobial activity from the Caspian Sea. Russ J Mar Biol 42, 36–41 (2016). https://doi.org/10.1134/S1063074016010089

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  • DOI: https://doi.org/10.1134/S1063074016010089

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