Abstract
This study examines the molecular species composition and heat-induced crystalline-liquid crystalline phase transitions of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) from the muscle tissues of six species of echinoderms that were collected during the summer: the starfishes Distolasterias nipon and Asterias amurensis, the sea urchin Strongylocentrotus intermedius, and the holothurians Eupentacta fraudatrix, Cucumaria frondosa japonica, and Apostichopus japonicus. Phospholipids (PLs) were in the liquid crystalline state, which is optimal for the functioning of the cell membranes. The use of data on the molecular species composition of PLs for the interpretation of their thermotropic behavior indicated that homeoviscous adaptation is achieved by various rearrangements in the composition of the aliphatic groups of PLs. The phase transitions of PC and PE of echinoderms (except holothurians) were symbatic. The presence of a high-temperature peak on the PC thermograms of C. frondosa japonica and A. japonicus is attributable to the melting of the phospholipid domain, which is composed of molecular species with saturated aliphatic groups. Such domains are responsible for a significant shift in the temperature ranges of the phase transitions of phospholipids of holothurians and sea urchin towards temperatures above 0°C.
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References
Ivkov, V.G. and Berestovskii, G.N., Dinamicheskaya struktura lipidnogo bisloya (Dynamical Structure of the Lipid Bilayer), Moscow: Nauka, 1981.
Gennis, R., Biomembranes: Molecular Structure and Function, New York: Springer-Verlag, 1989.
Kostetsky, E.Ya., Velansky, P.V., and Sanina, N.M., Phospholipids of the organs and tissues of echinoderms and tunicates from Peter the Great Bay (Sea of Japan), Russ. J. Mar. Biol., 2012, vol. 38, no. 1, pp. 65–71.
Kostetsky, E.Ya., Velansky, P.V., and Sanina, N.M., Individual variations of the phospholipid composition of the organs of arthropods, echinoderms, and tunicates from Peter the Great Bay of the Sea of Japan, Russ. J. Mar. Biol., 2012, vol. 38, no. 2, pp. 166–173.
Kostetsky, E.Ya., Velansky, P.V., and Sanina, N.M., Phase transitions of phospholipids as a criterion for assessing the capacity for thermal adaptation in fish, Russ. J. Mar. Biol., 2013, vol. 39, no. 2, pp. 136–143.
Kostetsky, E.Ya., Sanina, N.M., and Naumenko, N.V., Influence of the fatty acid composition upon the structure of the thermogram of the phase transition of phosphatidylcholine (PC) from the holothurian Cucumaria fraudatrix, Zh. Evol. Biochim. Fiziol., 1992, vol. 28, no. 4, pp. 426–433.
Kostetsky, E.Ya. and Sergeyuk, N.N., Influence of seasonal factors upon the content of phospholipids and their plasmalogenic forms in the musscle tissue of marine invertebrates, Zh. Evol. Biochim. Fiziol., 1986, vol. 22, no. 2, pp. 132–142.
Sanina, N.M., Membrane-forming lipids, physicochemical bases of thermoadaptation of marine invertebrates and macrophytes, Extended Abstract of Doctoral (Biol.) Dissertation, Vladivostok: Dal’nevost. Gos. Univ., 2006.
Kharakoz, D.P., On a possible biological role of the phase transition liquid-solid in biological membranes, Usp. Biol. Khim., 2001, vol.?41, pp. 333–364.
Brouwers, J.F.H.M., Vernooij, E.A.A.M., Tielens, A.G.M., and van Golde, L.M.G., Rapid separation and identification of phosphatidylethanolamine molecular species, J. Lipid Res., 1999, vol. 40, pp. 164–169.
Carboni, S., Hughes, A.D., Atack, T., et al., Fatty acid profiles during gametogenesis in sea urchin (Paracentrotus lividus): effects of dietary inputs on gonad, egg and embryo profiles, Comp. Biochem. Physiol. A, 2013, vol. 164, pp. 376–382.
Drazen, J.C., Phleger, C.F., Guest, M.A., and Nichols, P.D., Lipid, sterols and fatty acid composition of abyssal holothurians and ophiuroids from the North-East Pacific Ocean: food web implications, Comp. Biochem. Physiol. B, 2008, vol. 151, no. 1, pp. 79–87.
Fodor, E., Jones, R.H., Buda, C., et al., Molecular architecture and biophysical properties of phospholipids during thermal adaptation in fish: an experimental and model study, Lipids, 1995, vol. 30, no. 12, pp. 1119–1126.
Folch, J., Lees, M., and Sloane-Stangley, G.H., Asimple method for the isolation and purification of total lipids from animal tissues, J. Biol. Chem., 1957, vol. 226, no. 1, pp. 497–509.
Guschina, I.A. and Harwood, J.L., Mechanisms of temperature adaptation in poikilotherms, FEBS Lett., 2006, vol. 580, pp. 5477–5483.
Han, X. and Gross, R.W., Electrospray ionization mass spectroscopic analysis of human erythrocyte plasma membrane phospholipids, Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 10635–10639.
Han, X. and Gross, R.W., Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples, Mass Spectrom. Rev., 2005, vol. 24, pp. 367–712.
Hughes, A.D., Cook, E.J., Orr, H., et al., The transformation of long chain polyunsaturated fatty acids in benthic food webs: the role of sea urchins, J. Exp. Mar. Biol. Ecol., 2011, vol. 409, pp. 229–234.
Kanehisa, M.J. and Tsong, T.V., Cluster model of lipid phase transitions with application to passive permeation of molecules and structure relaxations in lipid bilayers, J. Am. Chem. Soc., 1978, vol. 18, pp. 424–432.
Logue, J.A., de Vries, A.L., Fodor, E., and Cossins, A.R., Lipid compositional correlates of temperature-adaptive interspecific differences in membrane physical structure, J. Exp. Biol., 2000, vol. 203, pp. 2105–2115.
Sanina, N.M. and Kostetsky, E.Ya., Seasonal changes in thermotropic behavior of phosphatidylcholine and phosphatidylethanolamine in different organs of the ascidian Halocynthia aurantium, Comp. Biochem. Physiol. B, 2001, vol. 128, pp. 295–305.
Sanina, N.M. and Kostetsky, E.Ya., Thermotropic behavior of major phospholipids from marine invertebrates: changes with warm-acclimation and seasonal acclimatization, Comp. Biochem. Physiol. B, 2002, vol. 133, no. 2, pp. 143–153.
Seddon, J.M. and Templer, R.H., Polymorphism of lipid-water system, in Structure and Dynamics of Membranes, Elsevier, 1995, pp. 97–161.
Tenchov, B.G., Nonuniform lipid distribution in membranes, Prog. Surf. Sci., 1985, vol. 20, no. 21, pp. 273–340.
Vecchini, A., Panagia, V., and Binagli, L., Analysis of phospholipid molecular species, Mol. Cell Biochem., 1997, vol. 172, pp. 129–136.
Wang, D., Xu, W., Xu, X., et al., Determination of intramuscular phospholipid classes and molecular species in Gaoyou duck, Food Chem., 2009, vol. 112, pp. 150–155.
Weaver, F.E., Shaikh, S.R., and Edidin, M., Plasma membrane lipid diffusion and composition of sea urchin egg membranes vary with ocean temperature, Chem. Phys. Lipids, 2008, vol. 151, no. 1, pp. 62–65.
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Original Russian Text © E.Ya. Kostetsky, N.M. Sanina, P.V. Velansky, 2014, published in Biologiya Morya.
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Kostetsky, E.Y., Sanina, N.M. & Velansky, P.V. The thermotropic behavior and major molecular species composition of the phospholipids of echinoderms. Russ J Mar Biol 40, 131–139 (2014). https://doi.org/10.1134/S1063074014020059
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DOI: https://doi.org/10.1134/S1063074014020059