Abstract
—Rapid and accurate bacterial identification plays a crucial role across diverse sectors, offering valuable applications. While traditional culture and molecular techniques maintain a high standard, the contemporary demand for diagnostics centers on swiftly delivering dependable insights into bacterial infections, directly at the site, within a short timeframe. In this study, we present a novel approach utilizing surface-enhanced Raman scattering (SERS)-active nanostructured substrates in the form of gold- and silver-coated porous silicon nanowires (AuAg@pSiNWs) for rapid and highly sensitive bacterial detection. The porous silicon nanowires are fabricated using a straightforward method known as metal-assisted chemical etching. Subsequently, silver and gold decoration is achieved through chemical reduction of metal salts, imbuing the substrates with SERS-active properties. Scanning electron microscopy data reveals that upon incubation with AuAg@pSiNWs, bacteria are localized amidst clusters and on the surface of the nanowires, particularly in the vicinity of bimetallic gold and silver nanoparticles. Illustrated through the utilization of Listeria innocua bacteria as a model, the SERS efficacy of AuAg@pSiNWs is highlighted, enabling rapid label-free bacterial diagnosis with a limit of detection of 1.14 × 104 CFU/mL.
REFERENCES
Rentschler, S., Kaiser, L., and Deigner, H.P., Int. J. Mol. Sci., 2021, vol. 22, no. 1, p. 456. https://doi.org/10.3390/ijms22010456
Lavrentev, F.V., Rumyantsev, I.S., Ivanov, A.S., Shilovskikh, V.V., Orlova, O.Y., Nikolaev, K.G., Andreeva, D.A., and Skorb, E.V., ACS Appl. Mater. Interfaces, 2022, vol. 14, no. 5, p. 7321. https://doi.org/10.1021/acsami.1c22470
Zakharzhevskii, M., Drozdov, A.S., Kolchanov, D.S., Shkodenko, L., and Vinogradov, V.V., Nanomaterials, 2020, vol. 10, no. 2, p. 313. https://doi.org/10.3390/nano10020313
Jarvis, R.M. and Goodacre, R., Anal. Chem., 2004, vol. 76, no. 1, p. 40. https://doi.org/10.1021/ac034689c
Pahlow, S., Meisel, S., Cialla-May, D., Weber, K., Rösch, P., and Popp, J., Adv. Drug Delivery Rev., 2015, vol. 89, p. 105. https://doi.org/10.1016/j.addr.2015.04.006
Fleischmann, M., Hendra, P.J., and McQuillan, A.J., Chem. Phys. Lett., 1974, vol. 26, no. 2, p. 163. https://doi.org/10.1016/0009-2614(74)85388-1
Schuster, K.C., Reese, I., Urlaub, E., Grapes, R., Lendl, B., Anal. Chem., 2000, vol. 72, p. 5529. https://doi.org/10.1021/ac000718x
Vendamani, V.S., Rao, S.N., Pathak, A.P., and Soma, V.R., ACS Appl. Nano Mater., 2022, vol. 5, no. 4, p. 4550. https://doi.org/10.1021/acsanm.1c04569
Gonchar, K.A., Zubairova, A.A., Schleusener, A., Osminkina, L.A., and Sivakov, V., Nanoscale Res. Lett., 2016, vol. 11, p. 357. https://doi.org/10.1186/s11671-016-1568-5
Huang, Z., Geyer, N., Werner, P., de Boor, J., and Gösele, U., Adv. Mater., 2011, vol. 23, p. 285. https://doi.org/10.1002/adma201001784
Gonchar, K.A., I. V. Bozh’ev, Shalygina, O.A., and Osminkina, L.A., JETP Lett., 2023, vol. 117, p. 111. https://doi.org/10.1134/S0021364022603098
Gongalsky, M.B., Pervushin, N.V., Maksutova, D.E., Tsurikova, U.A., Putintsev, P.P., Gyuppenen, O.D., Evstratova, Y.V., Shalygina, O.A., Kopeina, G.S., Kudryavtsev, A.A., and Osminkina, L.A., Nanomaterials, 2021, vol. 11, p. 2167. https://doi.org/10.3390/nano11092167
Gonchar, K.A., Moiseev, D.V., Bozhev, I.V., and Osminkina, L.A., Mater. Sci. Semicond. Proc., 2021, vol. 125, p. 105644. https://doi.org/10.1016/j.mssp.2020.105644
Žukovskaja, O., Agafilushkina, S., Sivakov, V., Weber, K., Cialla-May, D., Osminkina, L., and Popp, J., Talanta, 2019, vol. 202, p. 171. https://doi.org/10.1016/j.talanta.2019.04.047
Parker, J.H., Jr., Feldman, D.W., and Ashkin, M., Phys. Rev., 1967, vol. 155, no. 3, p. 712. https://doi.org/10.1103/PhysRev.155.712
Kartashova, A.D., Gonchar, K.A., Chermoshentsev, D.A., Alekseeva, E.A., Gongalsky, M.B., Bozhev, I.V., Eliseev, A.A., Dyakov, S.A., Samsonova, J.V., and Osminkina, L.A., ACS Biomater. Sci. Eng., 2022, vol. 8, no. 10, p. 4175. https://doi.org/10.1021/acsbiomaterials.1c00728
Gonchar, K.A., Alekseeva, E.A., Gyuppenen, O.D., Bozhev, I.V., Kalinin, E.V., Ermolaeva, S.A., and Osminkina, L.A., Opt. Spectrosc., 2022, vol. 130, p. 521. https://doi.org/10.1134/S0030400X22110017
Efrima, S. and Zeiri, L., J. Raman Spectrosc., 2009, vol. 40, no. 3, p. 277. https://doi.org/10.1002/jrs.2121
Potara, M., Jakab, E., Damert, A., Popescu, O., Canpean, V., and Astilean, S., Nanotechnology, 2011, vol. 22, no. 13, p. 135101. https://doi.org/10.1088/0957-4484/22/13/135101
Sundaram, J., Park, B., Kwon, Y., and Lawrence, K.C., Int. J. Food Microbiol., 2013, vol. 167, no. 1, p. 67. https://doi.org/10.1016/j.ijfoodmicro.2013.05.013
Uusitalo, S., Kögler, M., A.L. Välimaa, Popov, A., Ryabchikov, Y., Kontturi, V., Siitonen, S., Petäjä, J., Virtanen, T., Laitinen, R., and Kinnunen, M., RSC Adv., 2016, vol. 6, no. 67, p. 62981. https://doi.org/10.1039/C6RA08313G
Busch, R.T., Karim, F., Sun, Y., Fry, H.C., Liu, Y., Zhao, C., and Vasquez, E.S., Front. Nanotechnol., 2021, vol. 3, p. 653744. https://doi.org/10.3389/fnano.2021.653744
N. Massad-Ivanir, Shtenberg, G., Raz, N., Gazenbeek, C., Budding, D., Bos, M.P., and Segal, E., Sci. Rep., 2016, vol. 6, p. 1038. https://doi.org/10.1038/srep38099
Chiappini, C., Liu, X., Fakhoury, J.R., and Ferrari, M., Adv. Funct. Mater., 2010, vol. 20, no. 14, p. 2231. https://doi.org/10.1002/adfm.201000360
Premasiri, W.R., Lee, J.C., A. Sauer-Budge, Théberge, R., Costello, C.E., and Ziegler, L.D., Anal. Bioanal. Chem., 2016, vol. 408, p. 4631. https://doi.org/10.1007/s00216-016-9540-x
Maquelin, K., L.-P. Choo-Smith, T. van Vreeswijk, Endtz, H.Ph., Smith, B., Bennett, R., Bruining, H.A., and G. J. Puppels, Anal. Chem., 2000, vol. 72, no. 1, p. 12. https://doi.org/10.1021/ac991011h
Thode, J., How to determine the LOD using the calibration curve? https://mpl.loesungsfabrik.de/en/english-blog/method-validation/calibration-line-procedure.
ACKNOWLEDGMENTS
The measurements were performed using SEM of the Training Methodical Center of Lithography and Microscopy, Moscow State University.
Funding
The study was supported by the Russian Science Foundation, grant no. 22-72-10062. https://rscf.ru/en/project/22-72-10062/.
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Nazarovskaia, D.A., Domnin, P.A., Gyuppenen, O.D. et al. Advanced Bacterial Detection with SERS-Active Gold- and Silver-Coated Porous Silicon Nanowires. Bull. Russ. Acad. Sci. Phys. 87 (Suppl 1), S41–S46 (2023). https://doi.org/10.1134/S1062873823704385
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DOI: https://doi.org/10.1134/S1062873823704385