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Effect of Linoleic Acid on the Efficiency of Mouse Embryos’ Vitrification

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Abstract

The study examines the effect of in vitro cultivation of mouse embryos for 48 h with 200 µM linoleic acid (LA) on their viability after vitrification. Fluorescence microscopy of DAPI-stained embryos showed increased fragmentation of nuclei in case of exposure to LA without cryopreservation and a significant decrease in the number of cells in vitrified embryos 24 h after their thawing. Thus, in vitro cultivation with LA reduces the viability of mouse embryos after vitrification.

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ACKNOWLEDGMENTS

The authors express their gratitude to the Center for Genetic Resources of Laboratory Animals Shared-Access Center (http://spf.bionet.nsc.ru) and the Shared-Access Center for Microscopic Analysis of Biological Objects (Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences) (http://www.bionet.nsc.ru/microscopy).

Funding

The work was performed with the support of Russian Foundation for Basic Research, no. 19-016-00025, budget project no. 0259-2021-0015 with the usage of equipment of the Center for Genetic Resources of Laboratory Animals Shared-Access Center of the FRC Institute of Cytology and Genetics (Siberian Branch, Russian Academy of Sciences) supported by the Ministry of Science and Higher Education of Russia (Unique project ID is RFMEFI62119X0023).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    E. Yu. Brusentsev, T. N. Igonina, S. V. Okotrub, E. A. Chuyko & S. Ya. Amstislavsky

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  1. E. Yu. Brusentsev
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  2. T. N. Igonina
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  3. S. V. Okotrub
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  4. E. A. Chuyko
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  5. S. Ya. Amstislavsky
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Contributions

E.Yu. Brusentsev, T.N. Igonina, and S.Ya. Amstislavsky developed the design of the experiment. E.Yu. Brusentsev, T.N. Igonina, and S.V. Okotrub obtained preimplantation embryos, carried out their vitrification and thawing, and their cultivation in vitro. E.A. Chuyko carried out DAPI staining, counting the number of interphase nuclei, their fragments, and metaphase plates in developing blastocysts. E.Yu. Brusentsev, T.N. Igonina, S.V. Okotrub, and S.Ya. Amstislavsky participated in data analysis. E.Yu. Brusentsev, T.N. Igonina, and S.Ya. Amstislavsky participated in writing the text of the article. All the authors participated in the discussion of the results.

Corresponding author

Correspondence to S. Ya. Amstislavsky.

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Conflict of interest. The authors declare that they have no conflict of interests.

Statement on the welfare of animals. In the course of implementing this study, all manipulations performed with experimental animals, methods of anesthesia, euthanasia, and animal care before and after experimental interventions complied with international standards of bioethics.

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Translated by A. Ermakov

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Brusentsev, E.Y., Igonina, T.N., Okotrub, S.V. et al. Effect of Linoleic Acid on the Efficiency of Mouse Embryos’ Vitrification. Russ J Dev Biol 53, 51–54 (2022). https://doi.org/10.1134/S1062360422010027

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  • DOI: https://doi.org/10.1134/S1062360422010027

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