Abstract
To clarify the organizing effect of Semax and HLDF-6 peptides on the kinetics of protein synthesis in hepatocytes, in addition to an in vitro study (Brodsky et al., 2019), the effects of these peptides in vivo have been studied. The circahoralian (ultradian) rhythm of protein synthesis, that is, a marker of the direct cell-to-cell communication, was investigated in rats of different ages. Peptides were injected intraperitoneally into young (2–3-month-old) or old (1.5–2-year-old) rats at a 50–100-µg/kg dose. Hepatocytes were isolated and sparse or dense cultures were established. In sparse cultures from young rats that received one or another peptide, the rhythm of protein synthesis was observed; in the cultures from rats of the same age that were injected with saline, no rhythm was found. In dense cultures of old rats after the action of the peptide, the amplitudes of the rhythm of protein synthesis did not differ from the rhythms observed in young rats; after saline injection, the rhythm amplitudes were twice as low. Injection of the peptide into adult rats that had previously received dopamine caused a protein synthesis rhythm characteristic for rats of this age; the administration of dopamine abolished the rhythm. The synchronizing effect of the peptides was maintained for at least 2 days after their single administration to the rat. The use of Semax or HLDF-6 is recommended to compensate for the disturbances of the kinetics of protein synthesis in humans in aging and pathology.
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ACKNOWLEDGEMENTS
We are grateful to the Koltsov Institute of Developmental Biology, Russian Academy of Sciences, for providing access to the equipment.
Funding
This study was supported by the Russian Foundation for Basic Research (project no. 17-04-00460) and the state program according to the academic research plan of the Institute of Molecular Genetics of the Russian Academy of Sciences (project no. АААА-А19-119022590101-1).
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Brodsky, V.Y., Zolotarev, Y.A., Malchenko, L.A. et al. The Administration of Semax and HLDF-6 Peptides to Rats Regulates Protein Synthesis Rhythm in Hepatocytes and Corrects Senescent Disturbances. Russ J Dev Biol 51, 99–105 (2020). https://doi.org/10.1134/S1062360420020034
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DOI: https://doi.org/10.1134/S1062360420020034