Abstract
The results are presented on haploid androgenesis in Siberian sturgeon and sterlet induced by UV irradiation of ovicells. During irradiation, the cells in Ringer solution were rotated around a UV lamp. The efficiency of genetic inactivation of ovicells was estimated by the following parameters: manifestation of Hertwig effect, the fraction of embryos demonstrating haploid syndrome at final developmental stages, by arrest of embryonic development in hybrids Siberian sturgeon × great sturgeon, and by absence of maternal alleles of microsatellite loci in embryos. The dose-effect curve suggests that, during UV irradiation of ovicells of Siberian sturgeon, the complete genetic inactivation is attained at exposition of 120 s, while that in sterlet is 90 or 105 s. The results show a principal possibility of inactivation of ovicells by UV irradiation and use of such cells for producing androgenetic progeny of acipenserids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdel-Hakim, N.F., Soltan, M.A., and Bakeer, M.N., Induction of diploid androgenetic Nile tilapia (Oreochromis niloticus), Egypt. J. Aquat. Biol. Fish., 2000, vol. 4, no. 4, 337–349.
Arai, K., Masaoka, T., and Suzuki, R., Optimum conditions of UV ray irradiation for genetic inactivation of loach eggs, Nippon Suissan Gakkaishi, 1992, vol. 58, no. 7, 1197–1201.
Barmintseva, A.E. and Myuge, N.S., The use of microsatellite loci for identification of sturgeon species (Acipenseridae) and hybrid forms, Russ. J. Genet., 2013, vol. 49, no. 9, 950–961.
Birstein, V.J., Bemis, W.E., and Waldman, J.R., The threatened status of acipenseriform fishes: a summary, in Sturgeon Biodiversity and Conservation, Birstein, V.J., Eds., Dordrecht: Kluwer Acad. Publ., 1997, pp. 427–444.
Bongers, A.B.J., Veld, E.P.C., Abo, H.K., et al., Androgenesis in common carp (Cyprinus carpio L.) using UV irradiation in a synthetic ovarian fluid and heat shocks, Aquaculture, 1994, vol. 122, no. 2, 119–132.
Bongers, A.B.J., Nguenga, D., Eding, E.H., and Richter, C.J.J., Androgenesis in the African catfish, Clarias gariepinus, Aquat. Liv. Res., 1995, vol. 8, 329–332.
Christopher, J.G., Murugesan, A.G., and Sukumaran, N., Optimization of UV treatment to induce haploid androgenesis in the stinging catfish, Heteropneustes fossilis, Int. Aquatic. Res., 2012, vol. 4, no. 1, 1–8.
Corley-Smith, G.E. and Brandhorst, B.P., Preservation of endangered species and populations: a role for genome banking, somatic cell cloning, and androgenesis?, Mol. Reprod. Develop., 1999, vol. 53, 363–367.
David, C.J. and Marimuthu, G., Successful induction of intra-specific androgenesis in widow tetra, Gymnocorymbus ternetzi (Boulenger 1895) using UV irradiation, Asian Fisheries Sci., 2014, vol. 27, 117–126.
Ginzburg, A.S. and Detlaf, T.A., Razvitie osetrovykh ryb. Sozrevanie yaits, oplodotvorenie i embriogenez (Development of Sturgeon: Egg Maturation, Fertilization, and Embryogenesis), Moscow: Nauka, 1969.
Gomel’skii, B.I. and Recoubratsky, A.V., On the possibility of spontaneous androgenesis and gynogenesis in fish, Vopr. Ikhtiol., 1990, vol. 30, no. 4, 698–700.
Goncharov, B.F., Effect of the culture medium composition on the ability of sturgeon follicles to respond to gonadotropins by maturation, in Voprosy rannego ontogeneza ryb (Problems of Early Ontogenesis of Fish), Kiev: Nauk. Dumka, 1978, pp. 77–78.
Grunina, A.S. and Neyfakh, A.A., Induction of diploid androgenesis in Siberian sturgeon Acipenser baeri Brandt, Ontogenez, 1991, vol. 22, no. 1, 53–56.
Grunina, A.S. and Neyfakh, A.A., Induced diploid androgenesis, Physiol. Gen. Biol. Rev., 1997, vol. 12, 73–103.
Grunina, A.S. and Recoubratsky, A.V., Induced androgenesis in fish: obtaining viable nucleocytoplasmic hybrids, Russ. J. Dev. Biol., 2005, vol. 36, no. 4, 208–217.
Grunina, A.S., Recoubratsky, A.V., and Neyfakh, A.A., Induced diploid androgenesis in sturgeons, Sturgeon Quart., 1995, vol. 3, no. 3, 6–7.
Grunina, A.S., Recoubratsky, A.V., Tsvetkova, L.I., and Barmintsev, V.A., Investigation on dispermic androgenesis in sturgeon fish. The first successful production of androgenetic sturgeons with cryopreserved sperm, Int. J. Refrigeration, 2006, vol. 29, no. 3, 379–386.
Grunina, A.S., Recoubratsky, A.V., Barmintsev, V.A., et al., Dispermic androgenesis as a method for recovery of endangered sturgeon species, in Biology, Conservation and Sustainable Development of Sturgeons, Carmona, R., et al., Eds., Springer Sciences, 2009, pp. 187–204.
Henderson-Arzapalo, A. and King, T.L., Novel microsatellite markers for Atlantic sturgeon (Acipenser oxyrinchus) population delineation and broodstock management, Mol. Ecol. Notes, 2002, vol. 2, 437–439.
Hertwig, O., Die radium Krankheit tierischer keim zellen, Arch. Mikrosk. Anat., 1911, vol. 77, 1–97.
Ivanova, N.V. and Hebert, P.D.N., An inexpensive, automation friendly protocol for recovering high-quality DNA, Mol. Ecol. Notes, 2006, vol. 6, 998–1002.
Karayücel, I. and Karayücel, S., Optimisation of UV treatment duration to induce haploid androgenesis in the Nile tilapia (Oreochromis niloticus L.), Turk. J. Vet. Anim. Sci., 2003, vol. 27, 401–407.
Kirankumar, S. and Pandian, T.J., Production of androgenetic tiger berb, Puntius tetrazona, Aquaculture, 2003, vol. 228, 37–51.
Kowtal, G.V., Preliminary experiments in induction of polyploidy, gynogenesis, and androgenesis in the white sturgeon, Acipenser transmontanus Richardson, in Proc. World Symp. Selection, Hybridization Genet Engineering Aquaculture, Tiews, K., Ed., Berlin: Heenemann, 1987, vol. 2, 317–324.
Kucharczyk, D., Kucharczyk, D., Targonska, K., et al., Genetic inactivation of dace, Leuciscus leuciscus (L.), gametes using UV irradiation, Arch. Polish Fish., 2008, vol. 16, no. 4, 437–446.
Myers, J.M., Penman, D.J., Basavaraju, Y., et al., Induction of diploid androgenetic and mitotic gynogenetic Nile tilapia Oreochromis niloticus (L.), Theor. Appl. Genet., 1995, vol. 90, 205–210.
Recoubratsky, A.V., Grunina, A.S., Minin, A.A., et al., Dispermic androgenesis in Acipenser stellatus, Sturgeon Quart., 1996, vol. 4, no. 4, 12–14.
Recoubratsky, A.V., Grunina, A.S., Myuge, N.S., and Neyfakh, A.A., Production of androgenetic nucleocytoplasmic hybrids in surgeon fish, Russ. J. Dev. Biol., 1998, vol. 29, no. 4, 224–230.
Recoubratsky, A.V., Grunina, A.S., Barmintsev, V.A., et al., Meiotic gynogenesis in the stellate and Russian sturgeons and sterlet, Russ. J. Dev. Biol., 2003, vol. 32, no. 2, 92–101.
Rupert, C., Enzymatic photoreactivation, overview, in Molecular Mechanisms for Repair of DNA, New York: Plenum-Press, 1975, pp. 73–124.
Veprintsev, B.N. and Rott, N.N., Conserving genetic resources of animal species, Nature, 1979, vol. 280, 633–634.
Welsh, A.B., Blumberg, M., and May, B., Identification of microsatellite loci in lake sturgeon, Acipenser fulvescens, and their variability in green sturgeon, A. medirostris, Mol. Ecol. Notes, 2003, vol. 3, 47–55.
Zane, L. Ludwig, A., et al., Isolation and characterization of microsatellites in the Adriatic sturgeon (Acipenser naccarii), Mol. Ecol. Notes, 2002, vol. 2, 586–588.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © D.A. Balashov, E.V. Vinogradov, K.V. Kovalev, A.E. Barmintseva, A.V. Recoubratsky, A.S. Grunina, 2017, published in Ontogenez, 2017, Vol. 48, No. 5, pp. 386–396.
Rights and permissions
About this article
Cite this article
Balashov, D.A., Vinogradov, E.V., Kovalev, K.V. et al. Induced androgenesis in acipenserids may be obtained by ultraviolet radiation. Russ J Dev Biol 48, 330–339 (2017). https://doi.org/10.1134/S1062360417050022
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1062360417050022