Abstract
The genesis of wheat microsporial polyembryoids in vitro was analyzed in detail. The nature of different phenotypes of cereal polymeric embryos was identified. They represent the class “multiple shoot meristems,” which results from a cleavage polyembryony and is accompanied by organ fasciations of all known types (radial, flat, or ring). The morphological nature of cereal embryonic organs has been clarified: shoot meristem—axial organ; scutellum—lateral outgrowth of this axis; coleoptile—derivative of shoot meristem but fused with scutellum; terminality of scutellum—the result of linear fasciation that occurred historically. An explanation is given on how the structural model of an auxin polar transport works during the establishment of bilateral symmetry in a cereal embryo that is associated with the inverted polarization of the carrier protein PIN1 on cell membranes and, correspondingly, with the inverted auxin transport performed by this carrier (Fischer-Iglesias et al., 2001; Forestan et al., 2010).
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References
Batygina, T.B., Embriologiya pshenitsy (Wheat Embryology), Leningrad: Kolos, 1974.
Batygina, T.B., Stem cells and morphogenetic developmental programs in plants, Stem Cell Res. J., 2011, vol. 3, nos. 1–2, pp. 45–120.
Batygina T.B., Polymorphism of sexual and somatic embryos as manifestation of their developmental parallelism under natural conditions and in tissue culture, in Plant Biotechnology and Molecular Markers, Eds. Srivastava P.S., Narula A., Srivastava S. New Delhi: Anamaya Publishers, 2004. pp. 43–59.
Batygina, T.B., Biologiya razvitiya rastenii. Simfoniya zhizni (Developmental Biology of Plants: Symphony of Life), St. Petersburg: DEAN, 2014.
Batygina, T.B. and Osadtchiy, J.V., Polyembryony: twins—the result of vegetative propagation, Int. J. Plant Reprod. Biol., 2013, vol. 5, no. 1, pp. 21–27.
Batygina, T.B., Kruglova, N.N., Gorbunova, V.Yu., Titova, G.E., and Sel’dimirova, O.A., Ot mikrospory–k sortu (From Microspore to Variety), Moscow: Nauka, 2010.
Berlin, G.P. and Miksche, G.P., Botanical Microtechnique and Cytochemistry, Iowa: State Univ. Press, 1976.
Braun, A., Über Polyembryonie und Keimung von Coelebogyne, in Ein Naehtrag zu der Abhandlung über Parthenogenesis bei Pflanzen, Berlin: Abh. Kon. Akad. Wiss, 1859.
Brisibe, E.A., Gajdosova, A., Olesen, A., and Andersen, S.B., Cytodifferentiation and transformation of embryogenic callus lines derived from anther culture of wheat, J. Exp. Bot., 2000, vol. 51, no. 343, pp. 187–196.
Chuang, Ch.-Ch. and Ouyang, T.-W., A set of potato media for wheat anther culture, in Symp. on Plant Tissue Culture: Proceed., Peking: Sci. Press, 1978, pp. 52–56.
Choob, V.V. and Sinyushin, A.A., Flower and shoot fasciation: from phenomenology to the construction of models of apical meristem transformations, Russ. J. Plant Physiol., 2012, vol. 59, no. 4, pp. 530–545.
Danilova, M.F., On the nature of fasciation in plants, Bot. Zh., 1961, vol. 46, no. 10, pp. 1545–1549.
Durzan, D.J., Monozygotic cleavage polyembryogenesis and conifer tree improvement, Cytol. Genet., 2008, vol. 42, no. 3, pp. C. 27–447.
Erdelska, O. and Vidovencova, Z., Cleavage polyembryony in maize, Sex. Plant Reprod., 1992, vol. 5, no. 3, pp. 224–226.
Erdelska, O. and Vidovencova, Z., Cleavage polyembryony in vivo and in vitro, Biol. Plant., 1994, vol. 36, no. 3, pp. 329–334.
Ferguson, J.D. and McEwan, J.M., The chemical induction of supernumerary shoots in the developing embryos of wheat, Physiol. Plant., 1970, vol. 23, no. 1, pp. 18–28.
Ferguson, J.D., McEwan, J.M., and Card, K.A., Hormonally induced polyembryos in wheat, Physiol. Plant., 1979, vol. 45, no. 4, pp. 470–474.
Fischer-Iglesias, C., Sundberg, B., Neuhaus, G., and Jones, A.M., Auxin distribution and transport during embryonic pattern formation in wheat, Plant J., 2001, vol. 26, no. 2, pp. 115–129.
Fischer, C. and Neuhaus, G., Influence of auxin on the establishment of bilateral symmetry in monocots, Plant J., 1996, vol. 9, no. 5, pp. 659–669.
Fischer, C., Speth, V., Fleig-Eberenz, S., and Neuhaus, G., Induction of zygotic polyembryos in wheat: influence of auxin polar transport, Plant Cell, 1997, vol. 9, no. 10, pp. 1767–1780.
Forestan, C., Meda, S., and Varotto, S., ZmPIN1-mediated auxin transport is related to cellular differentiation during maize embryogenesis and endosperm development, Plant Physiol., 2010, vol. 152, no. 3, pp. 1373–1390.
Haccius, B., Experimentally induced twinning in plants, Nature, 1955, vol. 176, no. 4477, pp. 355–356.
Howell, S.H., Lall, S., and Che, P., Cytokinins and shoot development, Trends Plant Sci., 2003, vol. 8, no. 9, pp. 453–459.
Jackson, D. and Hake, S., Control of phyllotaxy in maize by the abphyl1 gene, Development, 1999, vol. 126, no. 2, pp. 315–323.
Kruglova, N.N. and Batygina, T.B., Metodicheskie rekomendatsii po ispol’zovaniyu morfogeneticheskogo potentsiala pyl’nika v biotekhnologicheskikh issledovaniyakh yarovoi myagkoi pshenitsy (Guidelines on the Use of the Morphogenetic Potential of the Anther in Biotechnological Studies of Spring Common Wheat), Ufa: IB UNTs RAN, 2002.
Kruglova, N.N., Batygina, T.B., Gorbunova, V.Yu., Titova, G.E., and Seldimirova, O.A., Embriologicheskie osnovy androklinii pshenitsy: atlas (Embryological Basis of Wheat Androcliny: Atlas), Moscow: Nauka, 2005.
Kruglova, N.N., Egorova, O.V., Seldimirova, O.A., Zaitsev, D.Yu., and Zinatullina, A.E., Svetovoi mikroskop kak instrument v biotekhnologii rastenii (The Light Microscope as a Tool in Plant Biotechnology), Ufa: Gilem, Bashk. Entsikl., 2013.
Lynn, J.A., Rapid toluidine blue staining of Epon-embedded and mounted “adjacent” sections, Amer. J. Clin. Path., 1965, vol. 44, no. 1, pp. 57–58.
Morgan, D.T. and Rappleye, R.D., Polyembryony in maize and lily following X-irradiation of the pollen, J. Hered., 1951, vol. 42, no. 2, pp. 91–93.
Nishimura, M., Comparative morphology and development of Poa pratensis, Phleum pretense and Setaria italica, Jap. J. Bot., 1922, vol. 1, no. 2, pp. 55–85.
Osone, K. and Oono, K., Effects of irradiation on embryogenesis in rice: I. Stage-specific responses in the induction of M1 lethality and twins, Jap. J. Breed., 1970, vol. 20, no. 3, pp. 151–159.
Paul, P., Awasthi, A., Kumar, S., Verma, S.K., Prasad, R., and Dhaliwal, H.S., Development of multiple embryos in polyembryonic insertional mutant OsPE of rice, Plant Cell Rep., 2012, vol. 31, no. 10, pp. 1779–1787.
Puri, A., Basha, P.O., Kumar, M., Rajpurohit, D., Randhawa, G.S., Kianian, S.F., Rishi, A., and Dhaliwal, H.S., The polyembryo gene (OsPE) in rice, Funct. Integr. Genom., 2010, vol. 10, no. 3, pp. 359–366.
Rober-Kleber, N., Albrechtová, J.T.P., Fleig, S., Huck, N., Michalke, W., Wagner, E., Speth, V., Neuhaus, G., and Fischer-Iglesias, C., Plasma membrane H+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development, Plant Physiol., 2003, vol. 131, no. 3, pp. 1302–1312.
Segui-Simarro, J.M. and Nuez, F., How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore-derived embryogenesis, Physiol. Plant., 2008, vol. 134, no. 1, pp. 1–12.
Segui-Simarro, J.M., Androgenesis revisited, Bot. Rev., 2010, vol. 76, pp. 377–404.
Seldimirova, O.A. and Kruglova, N.N., Androclinic embryoidogenesis in vitro in cereals, Biol. Bull. Rev., 2015, vol. 5, no. 2, pp. 156–165.
Seldimirova, O.A., Titova, G.E., Galin, I.R., and Kruglova, N.N., Structural mechanisms of symmetry establishment in microspore-derived embryoids in wheat: the data of scanning electron microscopy, Izv. Samarsk. NTs RAN, 2013, vol. 15, no. 3 (5), pp. 1676–1679.
Seldimirova, O.A., Titova, G.E., and Kruglova, N.N., Androclinic “Siamese embryos” of wheat in vitro, Izv. Ufimsk. NTs RAN, 2015, no. 4 (1), pp. 137–142.
Sharma, K.K. and Thorpe, T.A., Asexual embryogenesis in vascular plants in nature, in In Vitro Embryogenesis in Plants, Thorpe, T.A., Ed., Dordrecht: Kluwer Acad. Publ., 1995, pp. 17–72.
Soriano, M., Li, H., and Boutilier, K., Microspore embryogenesis: establishment of embryo identity and pattern in culture, Plant Reprod., 2013, vol. 26, pp. 181–196.
Titova, G.E., Structural organization of dicot and monocot embryos. Principles of differences and possible mechanisms of their origin, in XIX Intern. Congr. on Sexual Plant Reproduction: Abstr., Budapest, 2006, p. 94.
Titova, G.E., Structural organization of monocot and dicot embryos: principles of distinctions and possible mechanisms of their occurrence, in II mezhdunarodnaya shkola molodykh uchenykh “Embriologiya, genetika i biotekhnologiya”: Materialy (Proc. II Int. School for Young Scientists “Embryology, Genetics, and Biotechnology”), Ufa, 2007, pp. 112–114.
Yakovlev, M.S. and Snegirev, D.P., Effect of growth substances on the formation of polyembryonic caryopses in wheat, Bot. Zh., 1954, vol. 39, no. 2, pp. 187–194.
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Original Russian Text © G.E. Titova, O.A. Seldimirova, N.N. Kruglova, I.R. Galin, T.B. Batygina, 2016, published in Ontogenez, 2016, Vol. 47, No. 3, pp. 152–169.
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Titova, G.E., Seldimirova, O.A., Kruglova, N.N. et al. Phenomenon of “Siamese embryos” in cereals in vivo and in vitro: Cleavage polyembryony and fasciations. Russ J Dev Biol 47, 122–137 (2016). https://doi.org/10.1134/S10623604160300x61
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DOI: https://doi.org/10.1134/S10623604160300x61