Abstract
Possible involvement of MEK mitogen-activated protein kinase and TGF-β receptor in the processes of regeneration and morphogenesis in freshwater planarian flatworms Schmidtea mediterranea was studied using a pharmacological inhibitor analysis. It was found that pharmacological inhibitors of these kinases significantly inhibit the regeneration of the head end of the animals and that this effect is realized due to inhibition of proliferative activity of neoblasts, planarian stem cells. It is shown that that the inhibition of the studied protein kinases in regenerating planarians markedly disturbs stem cell differentiation and morphogenesis.
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References
Baguñá, J., Planarian neoblasts, Nature, 1981, no. 290, pp. 14–15.
Ermakova, O.N., Ermakov, A.M., and Tiras, Kh.P., Melatonin effect on the regeneration of the flatworm Girardia tigrina, Russ. J. Dev. Biol., 2009, vol. 40, no. 6, pp. 382–385.
Gentile, L., Cebrià, F., and Bartscherer, K., The planarian flatworm: an in vivo model for stem cell biology and nervous system regeneration, Dis. Model. Mech., 2011, vol. 4, no. 1, pp. 12–19.
Heldin, C.H., Landstrom, M., and Moustakas, A., Mechanism of TGF-beta signaling to growth arrest, apoptosis, and epithelial-mesenchymal transition, Curr. Opin. Cell Biol., 2009, vol. 21, pp. 166–176.
Kyriakis, J.M. and Avruch, J., Mammalian MAPK signal transduction pathways activated by stress and inflammation: a 10-year update, Physiol. Rev., 2012, vol. 92, no. 2, pp. 689–737.
Labbé, R.M., Irimia, M., Currie, K.W., et al., A comparative transcriptomic analysis reveals conserved features of stem cell pluripotency in planarians and mammals, Stem Cells, 2012, vol. 30, no. 8, pp. 1734–1745.
Newmark, P.A. and Sanchez Alvarado, A., Bromodeoxyuridine specifically labels the regenerative stem cells of planarians, Dev. Biol., 2000, vol. 220, pp. 142–153.
Onal, P., Grün, D., Adamidi, C., et al., Gene expression of pluripotency determinants is conserved between mammalian and planarian stem cells, EMBO J., 2012, vol. 31, no. 12, pp. 2755–2769.
Potekhina, E.S. and Nadezhdina, E.S., Mitogen-activated protein kinase cascades and participation of Ste20-like protein kinases in them, Usp. Biol. Khim., 2002, vol. 42, pp. 235–256.
Shibata, N., Rouhana, L., and Agata, K., Cellular and molecular dissection of pluripotent adult somatic stem cells in planarians, Dev. Growth Differ., 2010, vol. 52, pp. 27–41.
Tasaki, J., Shibata, N., Nishimura, O., et al., ERK signaling controls blastema cell differentiation during planarian regeneration, Development, 2011, vol. 138, no. 12, pp. 2417–2427.
Tiras, Kh.P. and Sakharova, N.Yu., Lifetime morphometry of planarians, Ontogenez, 1984, vol. 15, no. 1, pp. 42–48.
Umesono, Y., Tasaki, J., Nishimura, Y., et al., The molecular logic for planarian regeneration along the anteriorposterior axis, Nature, 2013, vol. 500, no. 7460, pp. 73–76.
Yang, S.H., Sharrocks, A.D., and Whitmarsh, A.J., Map kinase signalling cascades and transcriptional regulation, Gene, 2013, vol. 513, no. 1, pp. 1–13.
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Original Russian Text © A.M. Ermakov, O.N. Ermakova, S.A. Ermolaeva, 2014, published in Ontogenez, 2014, Vol. 45, No. 5, pp. 355–360.
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Ermakov, A.M., Ermakova, O.N. & Ermolaeva, S.A. Study of possible involvement of MEK mitogen-activated protein kinase and TGF-β receptor in planarian regeneration processes using pharmacological inhibition analysis. Russ J Dev Biol 45, 292–296 (2014). https://doi.org/10.1134/S106236041405004X
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DOI: https://doi.org/10.1134/S106236041405004X