Abstract
A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium chelator BAPTA-AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchronization in MSC consists in calcium-dependent phosphorylation of cell proteins.
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Original Russian Text © V.Ya. Brodsky, A.V. Vasil’ev, V.V. Terskikh, N.D. Zvezdina, V.I. Fateeva, L.A. Mal’chenko, E.V. Kiseleva, E.I. Bueverova, 2012, published in Ontogenez, 2012, Vol. 43, No. 3, pp. 229–232.
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Brodsky, V.Y., Vasil’ev, A.V., Terskikh, V.V. et al. Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal. Russ J Dev Biol 43, 196–198 (2012). https://doi.org/10.1134/S1062360412030022
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DOI: https://doi.org/10.1134/S1062360412030022