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Analysis of telomeric DNA: Current approaches and methods

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Abstract

Terminal parts of linear chromosomes in eukaryotes are represented by telomeric DNA and associated proteins. Length, sequence and structure are the major parameters of telomeric DNA. The present review describes a set of molecular, cellular and genetic methods used to study the parameters of telomeric DNA.

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References

  • A Complete Set of Human Telomeric Probes and Their Clinical Application. National Institutes of Health and Institute of Molecular Medicine Collaboration, Nat. Genet., 1996, vol. 14, pp. 86–89.

  • Ambrosini, A., Paul, S., Hu, S., et al., Human Subtelomeric Duplicon Structure and Organization, Genome Biol., 2007, vol. 8, p. R151.

    Article  PubMed  CAS  Google Scholar 

  • Anisimova, V.E., Rebrikov, D.V., Zhulidov, P.A., et al., Renaturation, Activation, and Practical Use of Recombinant Duplex-Specific Nuclease from Kamchatka Crab, Biochemistry (Mosc), 2006, vol. 71, pp. 513–519.

    Article  CAS  Google Scholar 

  • Armour, J.A., Sismani, C., Patsalis, P.C., et al., Measurement of Locus Copy Number by Hybridisation with Amplifiable Probes, Nucleic Acids Res., 2000, vol. 28, pp. 605–609.

    Article  PubMed  CAS  Google Scholar 

  • Bailey, S.M., Brenneman, M.A., and Goodwin, E.H., Frequent Recombination in Telomeric DNA May Extend the Proliferative Life of Telomerase-Negative Cells, Nucleic Acids Res., 2004, vol. 32, pp. 3743–3751.

    Article  PubMed  CAS  Google Scholar 

  • Bailey, S.M., Cornforth, M.N., Kurimasa, A., et al., Strand-Specific Postreplicative Processing of Mammalian Telomeres, Science, 2001, vol. 293, pp. 2462–2465.

    Article  PubMed  CAS  Google Scholar 

  • Bailey, S.M., Goodwin, E.H., and Cornforth, M.N., Strand-Specific Fluorescence in Situ Hybridization: The Co-Fish Family, Cytogenet. Genome Res., 2004, vol. 107, pp. 14–17.

    Article  PubMed  CAS  Google Scholar 

  • Baird, D.M., New Developments in Telomere Length Analysis, Exp. Gerontol., 2005, vol. 40, pp. 363–368.

    Article  PubMed  CAS  Google Scholar 

  • Baird, D.M., Rowson, J., Wynford-Thomas, D., et al., Extensive Allelic Variation and Ultrashort Telomeres in Senescent Human Cells, Nat. Genet., 2003, vol. 33, pp. 203–207.

    Article  PubMed  CAS  Google Scholar 

  • Ballif, B.C., Sulpizio, S.G., Lloyd, R.M., et al., The Clinical Utility of Enhanced Subtelomeric Coverage in Array CGH, Am. J. Med. Genet. A, 2007, vol. 143, pp. 1850–1857.

    Google Scholar 

  • Baroin, A., Prat, A., and Caron, F., Telomeric Site Position Heterogeneity in Macronuclear DNA of Paramecium Primaurelia, Nucleic Acids Res., 1987, vol. 15, pp. 1717–1728.

    Article  PubMed  CAS  Google Scholar 

  • Bates, P., Mergny, J.L., and Yang, D., Quartets in G-Major. The First International Meeting on Quadruplex DNA, EMBO Rep., 2007, vol. 8, pp. 1003–1010.

    PubMed  CAS  Google Scholar 

  • Bekaert, S., Derradji, H., and Baatout, S., Telomere Biology in Mammalian Germ Cells and During Development, Dev. Biol., 2004, vol. 274, pp. 15–30.

    Article  PubMed  CAS  Google Scholar 

  • Blackburn, E.H. and Challoner, P.B., Identification of a Telomeric DNA Sequence in Trypanosoma brucei, Cell, 1984, vol. 36, pp. 447–457.

    Article  PubMed  CAS  Google Scholar 

  • Blackburn, E.H. and Gall, J.G., A Tandemly Repeated Sequence at the Termini of the Extrachromosomal Ribosomal RNA Genes in Tetrahymena, J. Mol. Biol., 1978, vol. 120, pp. 33–53.

    Article  PubMed  CAS  Google Scholar 

  • Blasco, M.A., Telomeres and Human Disease: Ageing, Cancer and Beyond, Nat. Rev. Genet., 2005, vol. 6, pp. 611–622.

    Article  PubMed  CAS  Google Scholar 

  • Bollmann, F.M., Targeting Alt: The Role of Alternative Lengthening of Telomeres in Pathogenesis and Prevention of Cancer, Cancer Treat Rev., 2007, vol. 33, pp. 704–709.

    PubMed  CAS  Google Scholar 

  • Booth, C., Griffith, E., Brady, G., et al., Quantitative Amplification of Single-Stranded DNA (QAOS) Demonstrates That Cdc13-1 Mutants Generate sDNA in a Telomere to Centromere Direction, Nucleic Acids Res., 2001, vol. 29, pp. 4414–4422.

    Article  PubMed  CAS  Google Scholar 

  • Britt-Compton, B. and Baird, D.M., Intra-Allelic Mutation at Human Telomeres, Biochem. Soc. Trans., 2006, vol. 34, pp. 581–582.

    Article  PubMed  CAS  Google Scholar 

  • Britt-Compton, B., Rowson, J., Locke, M., et al., Structural Stability and Chromosome-Specific Telomere Length Is Governed by Cis-Acting Determinants in Humans, Hum. Mol. Genet., 2006, vol. 15, pp. 725–733.

    Article  PubMed  CAS  Google Scholar 

  • Brown, J., Saracoglu, K., Uhrig, S., et al., Subtelomeric Chromosome Rearrangements Are Detected Using an Innovative 12-Color Fish Assay (M-TEL), Nat. Med., 2001, vol. 7, pp. 497–501.

    Article  PubMed  CAS  Google Scholar 

  • Brown, W.R., Molecular Cloning of Human Telomeres in Yeast, Nature, 1989, vol. 338, pp. 774–776.

    Article  PubMed  CAS  Google Scholar 

  • Brown, W.R., Dobson, M.J., and MacKinnon, P., Telomere Cloning and Mammalian Chromosome Analysis, J. Cell. Sci., 1990, vol. 95(Pt 4), pp. 521–526.

    PubMed  CAS  Google Scholar 

  • Buckle, V.J. and Kearney, L., Untwirling Dirvish, Nat. Genet., 1993, vol. 5, pp. 4–5.

    Article  PubMed  CAS  Google Scholar 

  • Burge, S., Parkinson, G.N., Hazel, P., et al., Quadruplex DNA: Sequence, Topology and Structure, Nucleic Acids Res., 2006, vol. 34, pp. 5402–5415.

    Article  PubMed  CAS  Google Scholar 

  • Callicott, R.J. and Womack, J.E., Real-Time PCR Assay for Measurement of Mouse Telomeres, Comp. Med., 2006, vol. 56, pp. 17–22.

    PubMed  CAS  Google Scholar 

  • Caron, F., A High Degree of Macronuclear Chromosome Polymorphism Is Generated by Variable DNA Rearrangements in Paramecium primaurelia during Macronuclear Differentiation, J. Mol. Biol., 1992, vol. 225, pp. 661–678.

    Article  PubMed  CAS  Google Scholar 

  • Cawthon, R.M., Telomere Measurement by Quantitative Pcr, Nucleic Acids Res., 2002, vol. 30, pp. e47.

    Article  PubMed  Google Scholar 

  • Cesare, A.J. and Griffith, J.D., Telomeric DNA in ALT Cells Is Characterized by Free Telomeric Circles and Heterogeneous T-Loops, Mol. Cell Biol., 2004, vol. 24, pp. 9948–9957.

    Article  PubMed  CAS  Google Scholar 

  • Chai, W., Du, Q., Shay, J.W., et al., Human Telomeres Have Different Overhang Sizes at Leading Versus Lagging Strands, Mol. Cell, 2006, vol. 21, pp. 427–435.

    Article  PubMed  CAS  Google Scholar 

  • Chan, C.S. and Tye, B.K., Organization of DNA Sequences and Replication Origins at Yeast Telomeres, Cell, 1983, vol. 33, pp. 563–573.

    Article  PubMed  CAS  Google Scholar 

  • Cherry, J.M., Ball, C., Weng, S., et al., Genetic and Physical Maps of Saccharomyces cerevisiae, Nature, 1997, vol. 387, pp. 67–73.

    Article  PubMed  CAS  Google Scholar 

  • Cimino-Reale, G., Pascale, E., Battiloro, E., et al., The Length of Telomeric G-Rich Strand 3′-Overhang Measured by Oligonucleotide Ligation Assay, Nucleic Acids Res., 2001, vol. 29, pp. E35.

    Article  PubMed  CAS  Google Scholar 

  • Cornforth, M.N. and Eberle, R.L., Termini of Human Chromosomes Display Elevated Rates of Mitotic Recombination, Mutagenesis, 2001, vol. 16, pp. 85–89.

    Article  PubMed  CAS  Google Scholar 

  • Cross, S.H., Allshire, R.C., McKay, S.J., et al., Cloning of Human Telomeres by Complementation in Yeast, Nature, 1989, vol. 338, pp. 771–774.

    Article  PubMed  CAS  Google Scholar 

  • Davison, G.M., Telomeres and Telomerase in Leukaemia and Lymphoma, Transfus Apher Sci., 2007, vol. 37, pp. 43–47.

    Article  PubMed  Google Scholar 

  • de Bruin, D., Kantrow, S.M., Liberatore, R.A., et al., Telomere Folding Is Required for the Stable Maintenance of Telomere Position Effects in Yeast, Mol. Cell Biol., 2000, vol. 20, pp. 7991–8000.

    Article  PubMed  Google Scholar 

  • de Bruin, D., Zaman, Z., Liberatore, R.A., et al., Telomere Looping Permits Gene Activation by a Downstream Uas in Yeast, Nature, 2001, vol. 409, pp. 109–113.

    Article  PubMed  CAS  Google Scholar 

  • De Cian, A., Guittat, L., Kaiser, M., et al., Fluorescence-Based Melting Assays for Studying Quadruplex Ligands, Methods, 2007, vol. 42, pp. 183–195.

    Article  PubMed  CAS  Google Scholar 

  • de Lange, T., T-Loops and the Origin of Telomeres, Nat. Rev. Mol. Cell Biol., 2004, vol. 5, pp. 323–329.

    Article  PubMed  CAS  Google Scholar 

  • De Vries, B.B., Winter, R., Schinzel, A., et al., Telomeres: A Diagnosis at the End of the Chromosomes, J. Med. Genet., 2003, vol. 40, pp. 385–398.

    Article  PubMed  Google Scholar 

  • Deng, Z., Dheekollu, J., Broccoli, D., et al., The Origin Recognition Complex Localizes to Telomere Repeats and Prevents Telomere-Circle Formation, Curr Biol., 2007, vol. 17, pp. 1989–1995.

    Article  PubMed  CAS  Google Scholar 

  • Dijkwel, P.A. and Hamlin, J.L., Mapping Replication Origins by Neutral/Neutral Two-Dimensional Gel Electrophoresis, Methods, 1997, vol. 13, pp. 235–245.

    Article  PubMed  CAS  Google Scholar 

  • Dionne, I. and Wellinger, R.J., Cell Cycle-Regulated Generation of Single-Stranded G-Rich DNA in the Absence of Telomerase, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 13902–13907.

    Article  PubMed  CAS  Google Scholar 

  • Dixit, M., Ansseau, E., Tassin, A., et al., DUX4, a Candidate Gene of Facioscapulohumeral Muscular Dystrophy, Encodes a Transcriptional Activator of PITX1, Proc. Natl. Acad. Sci. USA, 2007, vol. 104, pp. 18157–18162.

    Article  PubMed  CAS  Google Scholar 

  • Dmitriev, P.V., Petrov, A.V., and Dontsova, O.A., Yeast Telosome Complex: Components and Their Functions, Biochemistry (Mosc), 2003, vol. 68, pp. 718–734.

    Article  CAS  Google Scholar 

  • Dutrillaux, B., [New System of Chromosome Banding: The T Bands (Author’s Transl)], Chromosoma, 1973, vol. 41, pp. 395–402.

    Article  PubMed  CAS  Google Scholar 

  • Eldridge, A.M. and Wuttke, D.S., Probing the Mechanism of Recognition of sDNA by the Cdc13-DBD, Nucleic Acids Res., 2008, vol. 36, pp. 1624–1633.

    Article  PubMed  CAS  Google Scholar 

  • Emery, H.S. and Weiner, A.M., An Irregular Satellite Sequence Is Found at the Termini of the Linear Extrachromosomal rDNA in Dictyostelium Discoideum, Cell, 1981, vol. 26, pp. 411–419.

    Article  PubMed  CAS  Google Scholar 

  • Epel, E.S., Blackburn, E.H., Lin, J., et al., Accelerated Telomere Shortening in Response to Life Stress, Proc. Natl. Acad. Sci. USA, 2004, vol. 101, pp. 17312–17315.

    Article  PubMed  CAS  Google Scholar 

  • Fajkus, J., Sykorova, E., and Leitch, A.R., Telomeres in Evolution and Evolution of Telomeres, Chromosome Res., 2005, vol. 13, pp. 469–479.

    Article  PubMed  CAS  Google Scholar 

  • Finelli, P., Fabris, S., Zagano, S., et al., Detection of T(4;14) (P16.3;Q32) Chromosomal Translocation in Multiple Myeloma by Double-Color Fluorescent in Situ Hybridization, Blood, 1999, vol. 94, pp. 724–732.

    PubMed  CAS  Google Scholar 

  • Fletcher, T.M., Sun, D., Salazar, M., et al., Effect of DNA Secondary Structure on Human Telomerase Activity, Biochemistry, 1998, vol. 37, pp. 5536–5541.

    Article  PubMed  CAS  Google Scholar 

  • Flint, J., Wilkie, A.O., Buckle, V.J., et al., The Detection of Subtelomeric Chromosomal Rearrangements in Idiopathic Mental Retardation, Nat. Genet., 1995, vol. 9, pp. 132–140.

    Article  PubMed  CAS  Google Scholar 

  • Freulet-Marriere, M.A., Potocki-Veronese, G., Deverre, J.R., et al., Rapid Method for Mean Telomere Length Measurement Directly from Cell Lysates, Biochem. Biophys. Res. Commun., 2004, vol. 314, pp. 950–956.

    Article  PubMed  CAS  Google Scholar 

  • Fujiwara, M., Kamma, H., Wu, W., et al., Alternative Lengthening of Telomeres in the Human Adrenocortical Carcinoma Cell Line H295R, Int. J. Oncol., 2006, vol. 29, pp. 445–451.

    PubMed  CAS  Google Scholar 

  • Galy, V., Olivo-Marin, J.C., Scherthan, H., et al., Nuclear Pore Complexes in the Organization of Silent Telomeric Chromatin, Nature, 2000, vol. 403, pp. 108–112.

    Article  PubMed  CAS  Google Scholar 

  • Garagna, S., Zuccotti, M., Capanna, E., et al., High-Resolution Organization of Mouse Telomeric and Pericentromeric DNA, Cytogenet. Genome Res., 2002, vol. 96, pp. 125–129.

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Cao, M., Gonzalo, S., Dean, D., et al., A Role for the Rb Family of Proteins in Controlling Telomere Length, Nat. Genet., 2002, vol. 32, pp. 415–419.

    Article  PubMed  CAS  Google Scholar 

  • Gardiner, J.M., Coe, E.H., and Chao, S., Cloning Maize Telomeres by Complementation in Saccharomyces cerevisiae, Genome, 1996, vol. 39, pp. 736–748.

    Article  PubMed  CAS  Google Scholar 

  • Gilson, E. and Geli, V., How Telomeres Are Replicated, Nat. Rev. Mol. Cell Biol., 2007, vol. 8, pp. 825–838.

    Article  PubMed  CAS  Google Scholar 

  • Gonzalo, S., Jaco, I., Fraga, M.F., et al., DNA Methyltransferases Control Telomere Length and Telomere Recombination in Mammalian Cells, Nat. Cell Biol., 2006, vol. 8, pp. 416–424.

    Article  PubMed  CAS  Google Scholar 

  • Goodwin, E. and Meyne, J., Strand-Specific Fish Reveals Orientation of Chromosome 18 Alphoid DNA, Cytogenet Cell Genet., 1993, vol. 63, pp. 126–127.

    Article  PubMed  CAS  Google Scholar 

  • Gowthaman, R., Krishnamoorthy, S., Nandakumar, R.D., et al., Teck Database: A Comprehensive Collection of Telomeric and Centromeric Sequences with Their Associated Proteins, Bioinformation, 2007, vol. 2, pp. 73–75.

    PubMed  Google Scholar 

  • Greider, C.W. and Blackburn, E.H., Identification of a Specific Telomere Terminal Transferase Activity in Tetrahymena Extracts, Cell, 1985, vol. 43, pp. 405–413.

    Article  PubMed  CAS  Google Scholar 

  • Griffith, J.D., Comeau, L., Rosenfield, S., et al., Mammalian Telomeres End in a Large Duplex Loop, Cell, 1999, vol. 97, pp. 503–514.

    Article  PubMed  CAS  Google Scholar 

  • Grudic, A., Jul-Larsen, A., Haring, S.J., et al., Replication Protein a Prevents Accumulation of Single-Stranded Telomeric DNA in Cells That Use Alternative Lengthening of Telomeres, Nucleic Acids Res., 2007, vol. 35, pp. 7267–7278.

    Article  PubMed  CAS  Google Scholar 

  • Guan, X.Y., Zhang, H., Bittner, M., et al., Chromosome Arm Painting Probes, Nat. Genet., 1996, vol. 12, pp. 10–11.

    Article  PubMed  CAS  Google Scholar 

  • Haaf, T. and Ward, D.C., Structural Analysis of Alpha-Satellite DNA and Centromere Proteins Using Extended Chromatin and Chromosomes, Hum. Mol. Genet., 1994, vol. 3, pp. 697–709.

    Article  PubMed  CAS  Google Scholar 

  • Hacia, J.G., Novotny, E.A., Mayer, R.A., et al., Design of Modified Oligodeoxyribonucleotide Probes to Detect Telomere Repeat Sequences in Fish Assays, Nucleic Acids Res., 1999, vol. 27, pp. 4034–4039.

    Article  PubMed  CAS  Google Scholar 

  • Hails, T., Huttner, O., and Day, A., Isolation of a Chlamydomonas reinhardtii Telomere by Functional Complementation in Yeast, Curr Genet., 1995, vol. 28, pp. 437–440.

    Article  PubMed  CAS  Google Scholar 

  • Han, H., Hurley, L.H., and Salazar, M., A DNA Polymerase Stop Assay for G-Quadruplex-Interactive Compounds, Nucleic Acids Res., 1999, vol. 27, pp. 537–542.

    Article  PubMed  CAS  Google Scholar 

  • Harley, C.B., Futcher, A.B., and Greider, C.W., Telomeres Shorten During Ageing of Human Fibroblasts, Nature, 1990, vol. 345, pp. 458–460.

    Article  PubMed  CAS  Google Scholar 

  • Harper, L., Golubovskaya, I., and Cande, W.Z., A Bouquet of Chromosomes, J. Cell. Sci., 2004, vol. 117, pp. 4025–4032.

    Article  PubMed  CAS  Google Scholar 

  • Harrington, L., Those Dam-Aged Telomeres!, Curr. Opin. Genet. Dev., 2004, vol. 14, pp. 22–28.

    Article  PubMed  CAS  Google Scholar 

  • Hashimoto, M., Kyo, S., Masutomi, K., et al., Analysis of Telomeric Single-Strand Overhang Length in Human Endometrial Cancers, FEBS Lett., 2005, vol. 579, pp. 2959–2964.

    Article  PubMed  CAS  Google Scholar 

  • Hayflick, L. and Moorhead, P.S., The Serial Cultivation of Human Diploid Cell Strains, Exp. Cell Res., 1961, vol. 25, pp. 585–621.

    Article  Google Scholar 

  • He, Y., Neumann, R.D., and Panyutin, I.G., Intramolecular Quadruplex Conformation of Human Telomeric DNA Assessed with 125I-Radioprobing, Nucleic Acids Res., 2004, vol. 32, pp. 5359–5367.

    Article  PubMed  CAS  Google Scholar 

  • Heacock, M., Spangler, E., Riha, K., et al., Molecular Analysis of Telomere Fusions in Arabidopsis: Multiple Pathways for Chromosome End-Joining, EMBO J., 2004, vol. 23, pp. 2304–2313.

    Article  PubMed  CAS  Google Scholar 

  • Hediger, F., Neumann, F.R., Van Houwe, G., et al., Live Imaging of Telomeres: yKu and Sir Proteins Define Redundant Telomere-Anchoring Pathways in Yeast, Curr. Biol., 2002, vol. 12, pp. 2076–2089.

    Article  PubMed  CAS  Google Scholar 

  • Hemann, M.T., Strong, M.A., Hao, L.Y., et al., The Shortest Telomere, Not Average Telomere Length, Is Critical for Cell Viability and Chromosome Stability, Cell, 2001, vol. 107, pp. 67–77.

    Article  PubMed  CAS  Google Scholar 

  • Henderson, E., Hardin, C.C., Walk, S.K., et al., Telomeric DNA Oligonucleotides Form Novel Intramolecular Structures Containing Guanine-Guanine Base Pairs, Cell, 1987, vol. 51, pp. 899–908.

    Article  PubMed  CAS  Google Scholar 

  • Heng, H.H. and Tsui, L.C., High Resolution Free Chromatin/DNA Fiber Fluorescent in situ Hybridization, J. Chromatogr. A, 1998, vol. 806, pp. 219–229.

    Article  PubMed  CAS  Google Scholar 

  • Henson, J.D., Hannay, J.A., McCarthy, S.W., et al., A Robust Assay for Alternative Lengthening of Telomeres in Tumors Shows the Significance of Alternative Lengthening of Telomeres in Sarcomas and Astrocytomas, Clin. Cancer Res., 2005, vol. 11, pp. 217–225.

    PubMed  CAS  Google Scholar 

  • Hockemeyer, D., Sfeir, A.J., Shay, J.W., et al., POT1 Protects Telomeres from a Transient DNA Damage Response and Determines how Human Chromosomes End, EMBO J., 2005, vol. 24, pp. 2667–2678.

    Article  PubMed  CAS  Google Scholar 

  • Horvath, M.P., Schweiker, V.L., Bevilacqua, J.M., et al., Crystal Structure of the Oxytricha Nova Telomere End Binding Protein Complexed with Single Strand DNA, Cell, 1998, vol. 95, pp. 963–974.

    Article  PubMed  CAS  Google Scholar 

  • Hultdin, M., Gronlund, E., Norrback, K., et al., Telomere Analysis by Fluorescence in Situ Hybridization and Flow Cytometry, Nucleic Acids Res., 1998, vol. 26, pp. 3651–3656.

    Article  PubMed  CAS  Google Scholar 

  • Ivessa, A.S., Zhou, J.Q., Schulz, V.P., et al., Saccharomyces Rrm3p, a 5′ to 3′ DNA Helicase That Promotes Replication Fork Progression through Telomeric and Subtelomeric DNA, Genes Dev., 2002, vol. 16, pp. 1383–1396.

    Article  PubMed  CAS  Google Scholar 

  • Jackson, S.A., Wang, M.L., Goodman, H.M., et al., Application of Fiber-Fish in Physical Mapping of Arabidopsis thaliana, Genome, 1998, vol. 41, pp. 566–572.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, J.E., Smith, J.S., Kozak, M.L., et al., In Vivo Veritas: Using Yeast to Probe the Biological Functions of G-Quadruplexes, Biochimie, 2008, vol. 90, pp. 1250–1263.

    Article  PubMed  CAS  Google Scholar 

  • Johnston, M., Hillier, L., Riles, L., et al., The Nucleotide Sequence of Saccharomyces cerevisiae Chromosome Xii, Nature, 1997, vol. 387, pp. 87–90.

    Article  PubMed  CAS  Google Scholar 

  • Kang, C., Zhang, X., Ratliff, R., et al., Crystal Structure of Four-Stranded Oxytricha Telomeric DNA, Nature, 1992, vol. 356, pp. 126–131.

    Article  PubMed  CAS  Google Scholar 

  • Kass-Eisler, A. and Greider, C.W., Recombination in Telomere-Length Maintenance, Trends Biochem. Sci., 2000, vol. 25, pp. 200–204.

    Article  PubMed  CAS  Google Scholar 

  • Kipling, D., Wilson, H.E., Thomson, E.J., et al., Yac Cloning Mus musculus Telomeric DNA: Physical, Genetic, in Situ and STS Markers for the Distal Telomere of Chromosome 10, Hum. mol. Genet., 1995, vol. 4, pp. 1007–1014.

    Article  PubMed  CAS  Google Scholar 

  • Kirwan, M. and Dokal, I., Dyskeratosis Congenita: A Genetic Disorder of Many Faces, Clin. Genet., 2008, vol. 73, pp. 103–112.

    Article  PubMed  CAS  Google Scholar 

  • Klobutcher, L.A., Swanton, M.T., Donini, P., et al., All Gene-Sized DNA Molecules in Four Species of Hypotrichs Have the Same Terminal Sequence and an Unusual 3′ Terminus, Proc. Natl. Acad. Sci. USA, 1981, vol. 78, pp. 3015–3019.

    Article  PubMed  CAS  Google Scholar 

  • Knight, S.J., Horsley, S.W., Regan, R., et al., Development and Clinical Application of an Innovative Fluorescence in Situ Hybridization Technique Which Detects Submicroscopic Rearrangements Involving Telomeres, Eur. J. Hum. Genet., 1997, vol. 5, pp. 1–8.

    PubMed  CAS  Google Scholar 

  • Knight, S.J., Lese, C.M., Precht, K.S., et al., An Optimized Set of Human Telomere Clones for Studying Telomere Integrity and Architecture, Am. J. Hum. Genet., 2000, vol. 67, pp. 320–332.

    Article  PubMed  CAS  Google Scholar 

  • Koch, J., Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-Prins, Methods Mol. Biol., 2006, vol. 334, pp. 81–88.

    PubMed  Google Scholar 

  • Koch, J.E., Kolvraa, S., Petersen, K.B., et al., Oligonucleotide-Priming Methods for the Chromosome-Specific Labelling of Alpha Satellite DNA in Situ, Chromosoma, 1989, vol. 98, pp. 259–265.

    Article  PubMed  CAS  Google Scholar 

  • Konig, P., Giraldo, R., Chapman, L., et al., The Crystal Structure of the DNA-Binding Domain of Yeast RAP1 in Complex with Telomeric DNA, Cell, 1996, vol. 85, pp. 125–136.

    Article  PubMed  CAS  Google Scholar 

  • Krejci, K. and Koch, J., Improved Detection and Comparative Sizing of Human Chromosomal Telomeres in Situ, Chromosoma, 1998, vol. 107, pp. 198–203.

    Article  PubMed  CAS  Google Scholar 

  • Krejci, K. and Koch, J., An in Situ Study of Variant Telomeric Repeats in Human Chromosomes, Genomics, 1999, vol. 58, pp. 202–206.

    Article  PubMed  CAS  Google Scholar 

  • Krejci, K., Stentoft, J., and Koch, J., Molecular Cytogenetics Investigation of the Telomeres in a Case of Philadelphia Positive B-ALL with a Single Telomere Expansion, Neoplasia, 1999, vol. 1, pp. 492–497.

    Article  PubMed  CAS  Google Scholar 

  • Kuimov, A.N., Polypeptide Components of Telomere Nucleoprotein Complex, Biochemistry (Mosc), 2004, vol. 69, pp. 117–129.

    Article  CAS  Google Scholar 

  • Lansdorp, P.M., Verwoerd, N.P., van de Rijke, F.M., et al., Heterogeneity in Telomere Length of Human Chromosomes, Hum. Mol. Genet., 1996, vol. 5, pp. 685–691.

    Article  PubMed  CAS  Google Scholar 

  • Laroche, T., Martin, S.G., Tsai-Pflugfelder, M., et al., The Dynamics of Yeast Telomeres and Silencing Proteins through the Cell Cycle, J. Struct. Biol., 2000, vol. 129, pp. 159–174.

    Article  PubMed  CAS  Google Scholar 

  • Larrivee, M., LeBel, C., and Wellinger, R.J., The Generation of Proper Constitutive G-Tails on Yeast Telomeres Is Dependent on the MRX Complex, Genes Dev., 2004, vol. 18, pp. 1391–1396.

    Article  PubMed  CAS  Google Scholar 

  • Lei, M., Podell, E.R., and Cech, T.R., Structure of Human POT1 Bound to Telomeric Single-Stranded DNA Provides a Model for Chromosome End-Protection, Nat. Struct. Mol. Biol., 2004, vol. 11, pp. 1223–1229.

    Article  PubMed  CAS  Google Scholar 

  • Lemmers, R.J., de Kievit, P., Sandkuijl, L., et al., Facioscapulohumeral Muscular Dystrophy Is Uniquely Associated with One of the Two Variants of the 4q Subtelomere, Nat. Genet., 2002, vol. 32, pp. 235–236.

    Article  PubMed  CAS  Google Scholar 

  • Lemmers, R.J., Wohlgemuth, M., van der Gaag, K.J., et al., Specific Sequence Variations within the 4q35 Region Are Associated with Facioscapulohumeral Muscular Dystrophy, Am. J. Hum. Genet., 2007, vol. 81, pp. 884–894.

    Article  PubMed  CAS  Google Scholar 

  • Lin, K.W. and Yan, J., The Telomere Length Dynamic and Methods of Its Assessment, J. Cell. Mol. Med., 2005, vol. 9, pp. 977–989.

    Article  PubMed  CAS  Google Scholar 

  • Louis, E.J. and Borts, R.H., A Complete Set of Marked Telomeres in Saccharomyces cerevisiae for Physical Mapping and Cloning, Genetics, 1995, vol. 139, pp. 125–136.

    PubMed  CAS  Google Scholar 

  • Maeshima, K., Janssen, S., and Laemmli, U.K., Specific Targeting of Insect and Vertebrate Telomeres with Pyrrole and Imidazole Polyamides, EMBO J., 2001, vol. 20, pp. 3218–3228.

    Article  PubMed  CAS  Google Scholar 

  • Makarov, V.L., Hirose, Y., and Langmore, J.P., Long G Tails at Both Ends of Human Chromosomes Suggest a C Strand Degradation Mechanism for Telomere Shortening, Cell, 1997, vol. 88, pp. 657–666.

    Article  PubMed  CAS  Google Scholar 

  • Makovets, S., Herskowitz, I., and Blackburn, E.H., Anatomy and Dynamics of DNA Replication Fork Movement in Yeast Telomeric Regions, Mol. Cell Biol., 2004, vol. 24, pp. 4019–4031.

    Article  PubMed  CAS  Google Scholar 

  • Masny, P.S., Bengtsson, U., Chung, S.A., et al., Localization of 4q35.2 to the Nuclear Periphery: Is FSHD a Nuclear Envelope Disease?, Hum. Mol. Genet., 2004, vol. 13, pp. 1857–1871.

    Article  PubMed  CAS  Google Scholar 

  • Masuno, M., Imaizumi, K., Nakamura, M., et al., Miller-Dieker Syndrome Due to Maternal Cryptic Translocation T(10;17) (Q26.3;P13.3), Am J. Med. Genet., 1995, vol. 59, pp. 441–443.

    Article  PubMed  CAS  Google Scholar 

  • Maxam, A.M. and Gilbert, W., Sequencing End-Labeled DNA with Base-Specific Chemical Cleavages, Methods Enzymol., 1980, vol. 65, pp. 499–560.

    Article  PubMed  CAS  Google Scholar 

  • McClintock, B., The Stability of Broken Ends of Chromosomes in Zea mays, Genetics, 1941, vol. 26, pp. 234–282.

    PubMed  CAS  Google Scholar 

  • Mefford, H.C. and Trask, B.J., The Complex Structure and Dynamic Evolution of Human Subtelomeres, Nat. Rev. Genet., 2002, vol. 3, pp. 91–102.

    Article  PubMed  CAS  Google Scholar 

  • Meselson, M. and Stahl, F.W., The Replication of DNA in Escherichia Coli, Proc. Natl. Acad. Sci. USA, 1958, vol. 44, pp. 671–682.

    Article  PubMed  CAS  Google Scholar 

  • Meyne, J., Baker, R.J., Hobart, H.H., et al., Distribution of Non-Telomeric Sites of the (TTAGGG)n Telomeric Sequence in Vertebrate Chromosomes, Chromosoma, 1990, vol. 99, pp. 3–10.

    Article  PubMed  CAS  Google Scholar 

  • Miller, K.M., Rog, O., and Cooper, J.P., Semi-Conservative DNA Replication through Telomeres Requires Taz1, Nature, 2006, vol. 440, pp. 824–828.

    Article  PubMed  CAS  Google Scholar 

  • Mitton-Fry, R.M., Anderson, E.M., Theobald, D.L., et al., Structural Basis for Telomeric Single-Stranded DNA Recognition by Yeast Cdc13, J. Mol. Biol., 2004, vol. 338, pp. 241–255.

    Article  PubMed  CAS  Google Scholar 

  • Molenaar, C., Wiesmeijer, K., Verwoerd, N.P., et al., Visualizing Telomere Dynamics in Living Mammalian Cells Using Pna Probes, EMBO J., 2003, vol. 22, pp. 6631–6641.

    Article  PubMed  CAS  Google Scholar 

  • Moyzis, R.K., Buckingham, J.M., Cram, L.S., et al., A Highly Conserved Repetitive DNA Sequence, (TTAGGG)n, Present at the Telomeres of Human Chromosomes, Proc. Natl. Acad. Sci. USA, 1988, vol. 85, pp. 6622–6626.

    Article  PubMed  CAS  Google Scholar 

  • Munoz-Jordan, J.L., Cross, G.A., de Lange, T., et al., T-Loops at Trypanosome Telomeres, EMBO J., 2001, vol. 20, pp. 579–588.

    Article  PubMed  CAS  Google Scholar 

  • Muntoni, A. and Reddel, R.R., The First Molecular Details of ALT in Human Tumor Cells, Hum. Mol. Genet., 2005, vol. 14.Spec No. 2, pp. R191–196.

    Article  PubMed  Google Scholar 

  • Nagele, R.G., Freeman, T., McMorrow, L., et al., Chromosomes Exhibit Preferential Positioning in Nuclei of Quiescent Human Cells, J. Cell. Sci., 1999, vol. 112(Pt. 4), pp. 525–535.

    PubMed  CAS  Google Scholar 

  • Nagele, R.G., Velasco, A.Q., Anderson, W.J., et al., Telomere Associations in Interphase Nuclei: Possible Role in Maintenance of Interphase Chromosome Topology, J. Cell. Sci., 2001, vol. 114, pp. 377–388.

    PubMed  CAS  Google Scholar 

  • Nakagawa, S., Gemmell, N.J., and Burke, T., Measuring Vertebrate Telomeres: Applications and Limitations, Mol. Ecol., 2004, vol. 13, pp. 2523–2533.

    Article  PubMed  CAS  Google Scholar 

  • Nakamura, Y., Hirose, M., Matsuo, H., et al., Simple, Rapid, Quantitative, and Sensitive Detection of Telomere Repeats in Cell Lysate by a Hybridization Protection Assay, Clin. Chem., 1999, vol. 45, pp. 1718–1724.

    PubMed  CAS  Google Scholar 

  • Nielsen, P.E., Egholm, M., Berg, R.H., et al., Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide, Science, 1991, vol. 254, pp. 1497–1500.

    Article  PubMed  CAS  Google Scholar 

  • Nishikawa, T., Okamura, H., Nagadoi, A., et al., Solution Structure of a Telomeric DNA Complex of Human TRF1, Structure, 2001, vol. 9, pp. 1237–1251.

    Article  PubMed  CAS  Google Scholar 

  • Oexle, K., Telomere Length Distribution and Southern Blot Analysis, J. Theor. Biol., 1998, vol. 190, pp. 369–377.

    Article  PubMed  CAS  Google Scholar 

  • Oexle, K. and Kohlschutter, A., Cause of Progression in Duchenne Muscular Dystrophy: Impaired Differentiation More Probable Than Replicative Aging, Neuropediatrics, 2001, vol. 32, pp. 123–129.

    Article  PubMed  CAS  Google Scholar 

  • Ohki, R. and Ishikawa, F., Telomere-Bound TRF1 and TRF2 Stall the Replication Fork at Telomeric Repeats, Nucleic Acids Res., 2004, vol. 32, pp. 1627–1637.

    Article  PubMed  CAS  Google Scholar 

  • Olovnikov, A.M., [Principle of Marginotomy in Template Synthesis of Polynucleotides], Dokl. Akad. Nauk SSSR, 1971, vol. 201, pp. 1496–1499.

    PubMed  CAS  Google Scholar 

  • Olovnikov, A.M., A Theory of Marginotomy. The Incomplete Copying of Template Margin in Enzymic Synthesis of Polynucleotides and Biological Significance of the Phenomenon, J. Theor. Biol., 1973, vol. 41, pp. 181–190.

    Article  PubMed  CAS  Google Scholar 

  • Paeschke, K., Simonsson, T., Postberg, J., et al., Telomere End-Binding Proteins Control the Formation of G-Quadruplex DNA Structures in Vivo, Nat. Struct. Mol. Biol., 2005, vol. 12, pp. 847–854.

    Article  PubMed  CAS  Google Scholar 

  • Panyutin, I.G., Kovalsky, O.I., Budowsky, E.I., et al., G-DNA: A Twice-Folded DNA Structure Adopted by Single-Stranded Oligo(Dg) and Its Implications for Telomeres, Proc. Natl. Acad. Sci. USA, 1990, vol. 87, pp. 867–870.

    Article  PubMed  CAS  Google Scholar 

  • Parkinson, G.N., Lee, M.P., and Neidle, S., Crystal Structure of Parallel Quadruplexes from Human Telomeric DNA, Nature, 2002, vol. 417, pp. 876–880.

    Article  PubMed  CAS  Google Scholar 

  • Parra, I. and Windle, B., High Resolution Visual Mapping of Stretched DNA by Fluorescent Hybridization, Nat. Genet., 1993, vol. 5, pp. 17–21.

    Article  PubMed  CAS  Google Scholar 

  • Patel, P.K. and Hosur, R.V., Nmr Observation of T-Tetrads in a Parallel Stranded DNA Quadruplex Formed by Saccharomyces Cerevisiae Telomere Repeats, Nucleic Acids Res., 1999, vol. 27, pp. 2457–2464.

    Article  PubMed  CAS  Google Scholar 

  • Phan, A.T. and Mergny, J.L., Human Telomeric DNA: G-Quadruplex, i-Motif and Watson-Crick Double Helix, Nucleic Acids Res., 2002, vol. 30, pp. 4618–4625.

    Article  PubMed  CAS  Google Scholar 

  • Ponzi, M., Pace, T., Dore, E., et al., Identification of a Telomeric DNA Sequence in Plasmodium Berghei, EMBO J., 1985, vol. 4, pp. 2991–2995.

    PubMed  CAS  Google Scholar 

  • Price, C.M. and Cech, T.R., Telomeric DNA-Protein Interactions of Oxytricha Macronuclear DNA, Genes Dev., 1987, vol. 1, pp. 783–793.

    Article  PubMed  CAS  Google Scholar 

  • Quina, A.S. and Parreira, L., Telomere-Surrounding Regions Are Transcription-Permissive 3D Nuclear Compartments in Human Cells, Exp. Cell Res., 2005, vol. 307, pp. 52–64.

    Article  PubMed  CAS  Google Scholar 

  • Rahman, R., Forsyth, N.R., and Cui, W., Telomeric 3′ Overhang Length Is Associated with the Size of Telomeres, Exp. Gerontol., 2008, vol. 43, pp. 258–265.

    Article  PubMed  CAS  Google Scholar 

  • Rezler, E.M., Bearss, D.J., and Hurley, L.H., Telomeres and Telomerases as Drug Targets, Curr. Opin. Pharmacol., 2002, vol. 2, pp. 415–423.

    Article  PubMed  CAS  Google Scholar 

  • Richards, E.J. and Ausubel, F.M., Isolation of a Higher Eukaryotic Telomere from Arabidopsis thaliana, Cell, 1988, vol. 53, pp. 127–136.

    Article  PubMed  CAS  Google Scholar 

  • Riethman, H., Closing in on Telomeric Closure, Genome Res., 1997, vol. 7, pp. 853–855.

    PubMed  CAS  Google Scholar 

  • Riethman, H., Ambrosini, A., Castaneda, C., et al., Mapping and Initial Analysis of Human Subtelomeric Sequence Assemblies, Genome Res., 2004, vol. 14, pp. 18–28.

    Article  PubMed  CAS  Google Scholar 

  • Riethman, H., Ambrosini, A., and Paul, S., Human Subtelomere Structure and Variation, Chromosome Res., 2005, vol. 13, pp. 505–515.

    Article  PubMed  CAS  Google Scholar 

  • Riethman, H.C., Moyzis, R.K., Meyne, J., et al., Cloning Human Telomeric DNA Fragments into Saccharomyces cerevisiae Using a Yeast-Artificial-Chromosome Vector, Proc. Natl. Acad. Sci. USA, 1989, vol. 86, pp. 6240–6244.

    Article  PubMed  CAS  Google Scholar 

  • Riethman, H.C., Xiang, Z., Paul, S., et al., Integration of Telomere Sequences with the Draft Human Genome Sequence, Nature, 2001, vol. 409, pp. 948–951.

    Article  PubMed  CAS  Google Scholar 

  • Riha, K., McKnight, T.D., Fajkus, J., et al., Analysis of the G-Overhang Structures on Plant Telomeres: Evidence for Two Distinct Telomere Architectures, Plant J., 2000, vol. 23, pp. 633–641.

    Article  PubMed  CAS  Google Scholar 

  • Riou, J.F., Guittat, L., Mailliet, P., et al., Cell Senescence and Telomere Shortening Induced by a New Series of Specific G-Quadruplex DNA Ligands, Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 2672–2677.

    Article  PubMed  CAS  Google Scholar 

  • Rosenberg, C., Florijn, R.J., Van de Rijke, F.M., et al., High Resolution DNA Fiber-Fish on Yeast Artificial Chromosomes: Direct Visualization of DNA Replication, Nat. Genet., 1995, vol. 10, pp. 477–479.

    Article  PubMed  CAS  Google Scholar 

  • Rosenberg, M., Hui, L., Ma, J., et al., Characterization of Short Tandem Repeats from Thirty-One Human Telomeres, Genome Res., 1997, vol. 7, pp. 917–923.

    PubMed  CAS  Google Scholar 

  • Rufer, N., Dragowska, W., Thornbury, G., et al., Telomere Length Dynamics in Human Lymphocyte Subpopulations Measured by Flow Cytometry, Nat. Biotechnol., 1998, vol. 16, pp. 743–747.

    Article  PubMed  CAS  Google Scholar 

  • Saccone, S., De Sario, A., Della Valle, G., et al., The Highest Gene Concentrations in the Human Genome Are in Telomeric Bands of Metaphase Chromosomes, Proc. Natl. Acad. Sci. USA, 1992, vol. 89, pp. 4913–4917.

    Article  PubMed  CAS  Google Scholar 

  • Sayegh, C., Jhunjhunwala, S., Riblet, R., et al., Visualization of Looping Involving the Immunoglobulin Heavy-Chain Locus in Developing B Cells, Genes Dev., 2005, vol. 19, pp. 322–327.

    Article  PubMed  CAS  Google Scholar 

  • Schaetzlein, S., Lucas-Hahn, A., Lemme, E., et al., Telomere Length Is Reset During Early Mammalian Embryogenesis, Proc. Natl. Acad. Sci. USA, 2004, vol. 101, pp. 8034–8038.

    Article  PubMed  CAS  Google Scholar 

  • Scherthan, H., Jerratsch, M., Li, B., et al., Mammalian Meiotic Telomeres: Protein Composition and Redistribution in Relation to Nuclear Pores, Mol. Biol. Cell, 2000, vol. 11, pp. 4189–4203.

    PubMed  CAS  Google Scholar 

  • Schoeftner, S. and Blasco, M.A., Developmentally Regulated Transcription of Mammalian Telomeres by DNA-Dependent RNA Polymerase Ii, Nat. Cell Biol., 2008, vol. 10, pp. 228–236.

    Article  PubMed  CAS  Google Scholar 

  • Schouten, J.P., McElgunn, C.J., Waaijer, R., et al., Relative Quantification of 40 Nucleic Acid Sequences by Multiplex Ligation-Dependent Probe Amplification, Nucleic Acids Res., 2002, vol. 30, pp. e57.

    Article  PubMed  Google Scholar 

  • Schrock, E., du Manoir, S., Veldman, T., et al., Multicolor Spectral Karyotyping of Human Chromosomes, Science, 1996, vol. 273, pp. 494–497.

    Article  PubMed  CAS  Google Scholar 

  • Sedivy, J.M., Shippen, D.E., and Shakirov, E.V., Surprise Ending, Nat. Genet., 2003, vol. 33, pp. 114–116.

    Article  PubMed  CAS  Google Scholar 

  • Serakinci, N. and Koch, J., Detection and Sizing of Telomeric Repeat DNA in Situ, Nat. Biotechnol., 1999, vol. 17, pp. 200–201.

    Article  PubMed  CAS  Google Scholar 

  • Serrano, A.L. and Andres, V., Telomeres and Cardiovascular Disease: Does Size Matter?, Circ. Res., 2004, vol. 94, pp. 575–584.

    Article  PubMed  CAS  Google Scholar 

  • Shampay, J., Szostak, J.W., and Blackburn, E.H., DNA Sequences of Telomeres Maintained in Yeast, Nature, 1984, vol. 310, pp. 154–157.

    Article  PubMed  CAS  Google Scholar 

  • Slijepcevic, P., Telomere Length Measurement by Q-FISH, Methods Cell Sci., 2001, vol. 23, pp. 17–22.

    Article  PubMed  CAS  Google Scholar 

  • Stewart, S.A., Ben-Porath, I., Carey, V.J., et al., Erosion of the Telomeric Single-Strand Overhang at Replicative Senescence, Nat. Genet., 2003, vol. 33, pp. 492–496.

    Article  PubMed  CAS  Google Scholar 

  • Sundquist, W.I. and Klug, A., Telomeric DNA Dimerizes by Formation of Guanine Tetrads between Hairpin Loops, Nature, 1989, vol. 342, pp. 825–829.

    Article  PubMed  CAS  Google Scholar 

  • Szostak, J.W. and Blackburn, E.H., Cloning Yeast Telomeres on Linear Plasmid Vectors, Cell, 1982, vol. 29, pp. 245–255.

    Article  PubMed  CAS  Google Scholar 

  • Taddei, A. and Gasser, S.M., Multiple Pathways for Telomere Tethering: Functional Implications of Subnuclear Position for Heterochromatin Formation, Biochim. Biophys. Acta, 2004, vol. 1677, pp. 120–128.

    PubMed  CAS  Google Scholar 

  • Tahara, H., Kusunoki, M., Yamanaka, Y., et al., G-Tail Telomere HPA: Simple Measurement of Human Single-Stranded Telomeric Overhangs, Nat. Methods, 2005, vol. 2, pp. 829–831.

    Article  PubMed  CAS  Google Scholar 

  • Tam, R., Smith, K.P., and Lawrence, J.B., The 4q Subtelomere Harboring the FSHD Locus Is Specifically Anchored with Peripheral Heterochromatin Unlike Most Human Telomeres, J. Cell Biol., 2004, vol. 167, pp. 269–279.

    Article  PubMed  CAS  Google Scholar 

  • Teng, S.C. and Zakian, V.A., Telomere-Telomere Recombination Is an Efficient Bypass Pathway for Telomere Maintenance in Saccharomyces cerevisiae, Mol. Cell Biol., 1999, vol. 19, pp. 8083–8093.

    PubMed  CAS  Google Scholar 

  • Therkelsen, A.J., Nielsen, A., Koch, J., et al., Staining of Human Telomeres with Primed in Situ Labeling (PRINS), Cytogenet. Cell Genet., 1995, vol. 68, pp. 115–118.

    Article  PubMed  CAS  Google Scholar 

  • Tupler, R. and Gabellini, D., Molecular Basis of Facioscapulohumeral Muscular Dystrophy, Cell Mol. Life Sci., 2004, vol. 61, pp. 557–566.

    Article  PubMed  CAS  Google Scholar 

  • Van der Ploeg, L.H., and Cornelissen, A.W., The Contribution of Chromosomal Translocations to Antigenic Variation in Trypanosoma brucei, Philos. Trans. R. Soc. Lond. B Biol. Sci., 1984, vol. 307, pp. 13–26.

    Article  PubMed  Google Scholar 

  • Veltman, J.A., Schoenmakers, E.F., Eussen, B.H., et al., High-Throughput Analysis of Subtelomeric Chromosome Rearrangements by Use of Array-Based Comparative Genomic Hybridization, Am. J. Hum. Genet., 2002, vol. 70, pp. 1269–1276.

    Article  PubMed  CAS  Google Scholar 

  • Vespa, L., Warrington, R.T., Mokros, P., et al., Atm Regulates the Length of Individual Telomere Tracts in Arabidopsis, Proc. Natl. Acad. Sci. USA, 2007, vol. 104, pp. 18145–18150.

    Article  PubMed  CAS  Google Scholar 

  • Walker, P.M.B., How Different Are the DNAs from Related Animals?, Nature, 1968, vol. 219, pp. 228–232.

    Article  PubMed  CAS  Google Scholar 

  • Wang, Y. and Patel, D.J., Solution Structure of the Human Telomeric Repeat D[AG3(T2AG3)3] G-Tetraplex, Structure, 1993, vol. 1, pp. 263–282.

    Article  PubMed  CAS  Google Scholar 

  • Watson, J.D. and Crick, F.H., Genetical Implications of the Structure of Deoxyribonucleic Acid, Nature, 1953, vol. 171, pp. 964–967.

    Article  PubMed  CAS  Google Scholar 

  • Weipoltshammer, K., Schofer, C., Almeder, M., et al., Intranuclear Anchoring of Repetitive DNA Sequences: Centromeres, Telomeres, and Ribosomal DNA, J. Cell Biol., 1999, vol. 147, pp. 1409–1418.

    Article  PubMed  CAS  Google Scholar 

  • Weitzmann, M.N., Woodford, K.J., and Usdin, K., The Development and Use of a DNA Polymerase Arrest Assay for the Evaluation of Parameters Affecting Intrastrand Tetraplex Formation, J. Biol. Chem., 1996, vol. 271, pp. 20958–20964.

    Article  PubMed  CAS  Google Scholar 

  • Wellinger, R.J., Ethier, K., Labrecque, P., et al., Evidence for a New Step in Telomere Maintenance, Cell, 1996, vol. 85, pp. 423–433.

    Article  PubMed  CAS  Google Scholar 

  • Wellinger, R.J., Wolf, A.J., and Zakian, V.A., Use of Non-Denaturing Southern Hybridization and Two Dimensional Agarose Gels to Detect Putative Intermediates in Telomere Replication in Saccharomyces cerevisiae, Chromosoma, 1992, vol. 102, pp. S150–156.

    Article  PubMed  CAS  Google Scholar 

  • Wellinger, R.J., Wolf, A.J., and Zakian, V.A., Origin Activation and Formation of Single-Strand TG1–3 Tails Occur Sequentially in Late S Phase on a Yeast Linear Plasmid, Mol. Cell Biol., 1993, vol. 13, pp. 4057–4065.

    PubMed  CAS  Google Scholar 

  • Wellinger, R.J., Wolf, A.J., and Zakian, V.A., Saccharomyces Telomeres Acquire Single-Strand TG1–3 Tails Late in S Phase, Cell, 1993, vol. 72, pp. 51–60.

    Article  PubMed  CAS  Google Scholar 

  • Wijmenga, C., Hewitt, J.E., Sandkuijl, L.A., et al., Chromosome 4q DNA Rearrangements Associated with Facioscapulohumeral Muscular Dystrophy, Nat. Genet., 1992, vol. 2, pp. 26–30.

    Article  PubMed  CAS  Google Scholar 

  • Williamson, J.R., Raghuraman, M.K., and Cech, T.R., Monovalent Cation-Induced Structure of Telomeric DNA: The G-Quartet Model, Cell, 1989, vol. 59, pp. 871–880.

    Article  PubMed  CAS  Google Scholar 

  • Wong, J.M. and Collins, K., Telomere Maintenance and Disease, Lancet, 2003, vol. 362, pp. 983–988.

    Article  PubMed  CAS  Google Scholar 

  • Wright, W.E., Tesmer, V.M., Huffman, K.E., et al., Normal Human Chromosomes Have Long G-Rich Telomeric Overhangs at One End, Genes Dev., 1997, vol. 11, pp. 2801–2809.

    Article  PubMed  CAS  Google Scholar 

  • Xu, L. and Blackburn, E.H., Human Cancer Cells Harbor T-Stumps, a Distinct Class of Extremely Short Telomeres, Mol. Cell, 2007, vol. 28, pp. 315–327.

    Article  PubMed  CAS  Google Scholar 

  • Yan, J., Bouchard, E.F., Samassekou, O., et al., Identification of a Human Chromosome-Specific Interstitial Telomere-Like Sequence (ITS) at 22q11.2 Using Double-Strand PRINS, Cytogenet. Genome Res., 2007, vol. 116, pp. 29–37.

    Article  PubMed  CAS  Google Scholar 

  • Yan, J., Chen, B.Z., Bouchard, E.F., et al., The Labeling Efficiency of Human Telomeres Is Increased by Double-Strand PRINS, Chromosoma, 2004, vol. 113, pp. 204–209.

    Article  PubMed  CAS  Google Scholar 

  • Yegorov, Y.E., Akimov, S.S., Akhmalisheva, A.K., et al., Blockade of Telomerase Function in Various Cells, Anticancer Drug Des., 1999, vol. 14, pp. 305–316.

    PubMed  CAS  Google Scholar 

  • Zahler, A.M., Williamson, J.R., Cech, T.R., et al., Inhibition of Telomerase by G-Quartet DNA Structures, Nature, 1991, vol. 350, pp. 718–720.

    Article  PubMed  CAS  Google Scholar 

  • Zaug, A.J., Podell, E.R., and Cech, T.R., Human POT1 Disrupts Telomeric G-Quadruplexes Allowing Telomerase Extension in Vitro, Proc. Natl. Acad. Sci. USA, 2005, vol. 102, pp. 10864–10869.

    Article  PubMed  CAS  Google Scholar 

  • Zhao, Y., Hoshiyama, H., Shay, J.W., et al., Quantitative Telomeric Overhang Determination Using a Double-Strand Specific Nuclease, Nucleic Acids Res., 2008, vol. 36, pp. e14.

    Article  PubMed  CAS  Google Scholar 

  • Zhdanova, N.S., Rubtsov, N.B., and Minina, Yu.M., Terminal Regions of Mammal Chromosomes: Plasticity and Role in Evolution, Genetika, 2007, vol. 43, pp. 873–886.

    PubMed  CAS  Google Scholar 

  • Zijlmans, J.M., Martens, U.M., Poon, S.S., et al., Telomeres in the Mouse Have Large Inter-Chromosomal Variations in the Number of T2ag3 Repeats, Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 7423–7428.

    Article  PubMed  CAS  Google Scholar 

  • Zou, Y., Gryaznov, S.M., Shay, J.W., et al., Asynchronous Replication Timing of Telomeres at Opposite Arms of Mammalian Chromosomes, Proc. Natl. Acad. Sci. USA, 2004, vol. 101, pp. 12928–12933.

    Article  PubMed  CAS  Google Scholar 

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Original Russian Text © P.V. Dmitriev, Y.S. Vassetzky, 2009, published in Ontogenez, 2009, vol. 39, No. 3, pp. 163–184.

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Dmitriev, P.V., Vassetzky, Y.S. Analysis of telomeric DNA: Current approaches and methods. Russ J Dev Biol 40, 125–144 (2009). https://doi.org/10.1134/S1062360409030011

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