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The Effect of the Probiotic “Subtilis-C” on the Lactoferrin Content in the Blood and Epidermal Secretions of “Feeding” Discus Fish

  • ANIMAL AND HUMAN PHYSIOLOGY
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Abstract

Probiotics modulate the immune response, displace pathogenic microorganisms from the gastrointestinal tract, and increase the survival rate of larvae. The object of this study were discus fish feeding their offspring with an epidermal secretion similar in a set of components to mammalian milk. Lactoferrin is found in the blood and skin secretions of the discus; it has antibacterial, antifungal, antiviral, and antiparasitic activity. The addition of the probiotic “Subtilis-C” to the diet of the feeding discus increases the production of lactoferrin in the epidermal secretions of the parents and also contributes to better survival of larvae and fry of fish.

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REFERENCES

  1. Actor, J.K., Hwang, S.A., and Kruzel, M.L., Lactoferrin as a natural immune modulator, Curr. Pharm. Des., 2009, no. 15, pp. 1956–1973.

  2. Banerjee, G. and Ray, A.K., Bacterial symbiosis in the fish gut and its role in health and metabolism, Symbiosis, 2017, no. 72 (1), pp. 1–11.

  3. Bennett, R.M. and Kokocinski, T., Lactoferrin content of peripheral blood cells, Br. J. Haematol., 2005, no. 39, pp. 509–521.

  4. Chevalier, C., Stojanovic, O., Colin, D.J., Suarez-Zamorano, N., Tarallo, V., Veyrat-Durebex, C., Rigo, D., Fabbiano, S., Stevanovic, A., Hagemann, S., Montet, S., Seimbille, Y., Zamboni, N., Hapfelmeier, S., and Trajkovski, M., Gut microbiota orchestrates energy homeostasis during cold, Cell, 2015, no. 163 (6), pp. 1360–1374.

  5. Chong, K., Ying, T.S., Foo, J., Jin, L.T., and Chong, A., Characterization of proteins in epidermal mucus of discus fish (Symphysodon spp.) during parental phase, Aquaculture, 2005, no. 249, pp. 469–476.

  6. Chong, K., Joshi, S., Jin, L.T., and Chong, A., Proteomics profiling of epidermal mucus secretion of a cichlid (Symphysodon aequifasciata) demonstrating parental care behavior, Proteomics, 2006, no. 5, pp. 2251–2258.

  7. Esteban, M.A., Rodríguez, A., Meseguer, C., and José, A., Effects of lactoferrin on non-specific immune responses of gilthead seabream (Sparus auratus L.), Fish Shellfish Immunol., 2005, no. 18 (2), pp. 109–124.

  8. Fernandes, K.E. and Carter, D.A., The antifungal activity of lactoferrin and its derived peptides: mechanisms of action and synergy with drugs against fungal pathogens, Front. Microbiol., 2017, no. 8, pp. 1–6.

  9. Giansanti, F., Leboffe, L., D’Elia, I., and Antonini, G., An update on the antifungal activities of lactoferrin: new promising applications in diagnostic, therapeutics and biotechnology, Anti-Infect. Agents, 2013, no. 11, pp. 155–158.

  10. Giansanti, F., Panella, G., Leboffe, L., and Antonini, G., Lactoferrin from milk: nutraceutical and pharmacological properties, Pharmaceuticals (Basel), 2016, no. 9 (4), pp. 1–15.

  11. Khong, H.K., Kuah, M.K., Jaya-Ram, A., and Shu-Chien, A.C., Prolactin receptor mRNA is upregulated in discus fish (Symphysodon aequifasciata) skin during parental phase, Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol., 2009, no. 153, pp. 18–28.

  12. Legrand, D. and Mazurier, J., A critical review of the roles of host lactoferrin in immunity, Biometals, 2010, no. 23, pp. 365–376.

  13. Popova, E.N., Orlova, A.S., Ponomarev, A.B., Zakrevskii, V.V., and Sergeev, V.N., The possibility of probiotic correction of maternal microflora disorders to prevent possible complications in the child. Influence of the mother’s microflora on the formation of the health of the unborn child, in Mikrobiota (Microbiota), Moscow: Media Sfera, 2019, pp. 140–152.

    Google Scholar 

  14. Possemiers, S., Bolca, S., Verstraete, W., and Heyerick, A., The intestinal microbiome: a separate organ inside the body with the metabolic potential to influence the bioactivity of botanicals, Fitoterapia, 2011, no. 82 (1), pp. 53–66.

  15. Postnikova, L.A., Noniashvili, E.M., and Patkin, E.L., The effect of lactoferrin on the development of early embryos of mice, with intrauterine exposure to bisphenol A, in Sb. nauchnykh trudov Vseros. nauch.-prakt. konf. s mezhdunarodnym uchastiem “Sovremennye dostizheniya khimiko-biologicheskikh nauk v profilakticheskoi i klinicheskoi meditsine” (Proc. All-Russ. Sci.-Pract. Conf. with International Participation “Modern Advances of Chemical and Biological Sciences in Preventive and Clinical Medicine”), Silin, A.V. and Gaikova, L.B., Ed., St. Petersburg: SZGMU im. I.I. Mechnikova, 2020, part 2, pp. 171–176.

  16. Sandomirsky, B.P., Galchemko, S.E., and Galchenko, K.S., Antioxidative properties of lactoferrin from bovine colostrums before and after its lyophilization, Cryoletters, 2003, vol. 24, pp. 275–280.

    CAS  PubMed  Google Scholar 

  17. Satoh, S., Tanoue, H., and Mohri, M., Costs and benefits of biparental mucus provisioning in discus fish (Symphysodon aequifasciatus), Ichthyol. Res., 2018, no. 65, pp. 510–514.

  18. Sayes, C., Leyton, Y., and Riquelme, C., Probiotic bacteria as a healthy alternative for fish aquaculture, in Antibiotic Use in Animals, London: IntechOpen, 2018, pp. 115–132.

    Google Scholar 

  19. Sherman, M.P., Bennett, S.H., Hwang, F.F., and Yu, C., Neonatal small bowel epithelia: enhancing anti-bacterial defense with lactoferrin and Lactobacillus GG, Biometals, 2004, no. 17, pp. 285–289.

  20. Sylvain, F. and Derome, N., Vertically and horizontally transmitted microbial symbionts shape the gut microbiota ontogenesis of a skin-mucus feeding discus fish progeny, Sci. Rep., 2017, no. 7 (1), pp. 52–63.

  21. Tarnecki, A.M., Wafapoor, M., Phillips, R.N., and Rhody, N.R., Benefits of a Bacillus probiotic to larval fish survival and transport stress resistance, Sci. Rep., 2019, no. 9, pp. 1–11.

  22. Turin, C.G., Zea, A., Pezo, A., Cruz, K., Zegarra, J., Bellomo, S., Cam, L., Llanos, R., Castañeda, A., Tucto, L., and Ochoa, T.J., Lactoferrin for prevention of neonatal sepsis, Biometals, 2014, no. 27 (5), pp. 1007–1016.

  23. Wang, Y.Z., Xu, C.L., An, Z.H., Liu, J.X., and Feng, J., Effect of dietary bovine lactoferrin on performance and antioxidant status of piglets, Anim. Feed Sci. Technol., 2008, vol. 140, pp. 326–336.

    Article  CAS  Google Scholar 

  24. Xiong, J., Nie, L., and Chen, J., Current understanding on the roles of gut microbiota in fish disease and immunity, Zool. Res., 2019, no. 40 (2), pp. 70–76.

  25. Zhang, Y., Wen, B., Meng, L.-J., Gao, J.-Z., and Chen, Z.-Zh., Dynamic changes of gut microbiota of discus fish (Symphysodon haraldi) at different feeding stages, Aquaculture, 2021, vol. 531, pp. 1–9.

    Article  Google Scholar 

  26. Zorina, V.N., Structure and inhibitory activity of lactoferrin in relation to the influenza virus, Infekts. Immun., 2020, no. 10 (1), pp. 49–54.

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Funding

This work was supported by the Russian Foundation for Basic Research, project no. 20-316-90028.

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Authors and Affiliations

  1. Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, 127550, Moscow, Russia

    G. I. Pronina & O. V. Sanaya

  2. Pharmoborona Testing Center, Ltd., 141074, Korolev, Moscow oblast, Russia

    A. O. Revyakin

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  1. G. I. Pronina
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  2. O. V. Sanaya
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  3. A. O. Revyakin
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Correspondence to G. I. Pronina.

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Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Pronina, G.I., Sanaya, O.V. & Revyakin, A.O. The Effect of the Probiotic “Subtilis-C” on the Lactoferrin Content in the Blood and Epidermal Secretions of “Feeding” Discus Fish. Biol Bull Russ Acad Sci 50, 47–51 (2023). https://doi.org/10.1134/S1062359023010107

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  • DOI: https://doi.org/10.1134/S1062359023010107

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