Abstract
The relevance of the work is related to the need to develop methods for determining individual opportunities to minimize the harmful effects of ionizing radiation. Experiments were conducted to search for informative indicators of the structure of mast cells to predict the individual effectiveness of the radioprotective effect of hypoxia. A study was performed on white male rats. In 81 animals, before the main experiment, a hypoxic sample was carried out once, by placing the rats in a gas mixture containing 8% oxygen and 92% nitrogen (GGS-8). In each of these rats, a biopsy of the skin of the auricle was performed immediately before the test and at the time of its completion. In the biopsies, the content of various forms of mast cells detected by the method of metachromasia with toluidine blue was determined. After a week, all animals of the main group (81 rats) were exposed to total γ-irradiation at a dose of 11 Gy under the protection of GGS-8. Rats in the control group (63 rats) were irradiated in the same dose in the air. The life expectancy after the exposure was assessed. The radioprotective effect of the gas hypoxic mixture was confirmed, which is expressed in an increase in the average life expectancy of irradiated rats by 2.2 times. A weak relationship between individual quantitative characteristics of mast cells and the life expectancy of irradiated animals is shown. A formal expression of the equation of regression of the number of mast cells on the life expectancy of animals irradiated under the protection of hypoxia has been created. On the basis of regression analysis, groups of animals in which the highest radioprotective effect of hypoxia was manifested were revealed. The minimum life expectancy after irradiation is characteristic of rats with extreme values of the mast cell reactivity index, and all surviving cases are noted in rats with average values of the indicator. Among them there was not a single animal with an intestinal form of death. This indicates the possibility of predicting the radioprotective effect of hypoxia based on an assessment of the functional activity of mast cells.
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Statement on the welfare of animals. The experiments were performed at the State Research Center for Aviation and Space Medicine (SRCASM) in compliance with national and international requirements for housing and humane treatment of animals. The experiments were approved by the committee for bioethics of SRCASM.
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Ushakov, I.B., Kordenko, A.N. Use of the Method of Assessing the Secretoma of Skin Mast Cells to Determine the Possible Individual Radiomodifying Effect of Hypoxia. Biol Bull Russ Acad Sci 49, 2309–2313 (2022). https://doi.org/10.1134/S1062359022120238
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DOI: https://doi.org/10.1134/S1062359022120238