Abstract
The combined effect of protective agents (helium-neon laser, ibuprofen, mexidol) and 3 Gy of accelerated carbon ions (12C) on the cognitive abilities of mice was studied. It was shown that the irradiated animals did not exhibit an altered behavior pattern: the level of anxiety was not increased, there was a slower positive dynamics of learning compared to the control, and there was no deficit in the hippocampus-dependent memory. Analysis of variance of learning curves revealed different coefficients of skill acquisition within the experimental groups, with the lowest characteristic for the group irradiated with a dose of 3 Gy 12C without protective agents. In addition, the analysis of the preference for novelty when testing for recognition of a new object showed that this group of animals has a violation of the nonspatial hippocampus-mediated short-term memory.
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ACKNOWLEDGMENTS
The authors thank E.N. Smirnova and A.E. Shemyakov, staff of the Laboratory of Cellular Engineering, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences (Pushchino) for their assistance in organizing and conducting the experiment on irradiation with accelerated carbon ions on the U-70 accelerator complex (Institute of High Energy Physics, National Research Center Kurchatov Institute, Protvino).
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Statement on the welfare of animals. This study was approved by the Commission on Biological Safety and Bioethics of the Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences (protocol no. 23/2019). The experiments were carried out in accordance with the requirements of the Federation of European Laboratory Animal Science Associations (FELASA).
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Translated by N. Smolina
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Sorokina, S.S., Paskevich, S.I., Zaichkina, S.I. et al. The Combined Effect of Protective Agents and Accelerated Carbon Ions on the Behavior of Mice. Biol Bull Russ Acad Sci 49, 2303–2308 (2022). https://doi.org/10.1134/S1062359022120226
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DOI: https://doi.org/10.1134/S1062359022120226