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Influence of Gamma Irradiated Steinernema carpocapsae on Some Physiological Aspects of Galleria mellonella Larvae

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The physiological changes in G. mellonella larvae treated with unirradiated and 2 Gy gamma irradiated S. carpocapsae had been investigated. The results indicated that S. carpocapsae exhibited a significant decrease in the total body concentrations of proteins, lipids, carbohydrates and urea of 5th larval of G. mellonella with increasing the infection incubation period. Moreover, the decrease in the concentration of these total body metabolic products was greatly remarkable in larvae infected with gamma irradiated S. carpocapsae. In contrast, the results revealed that S. carpocapsae caused a significant increase in the acid phospatase and chitinase activity of the total body of 5th larval instar of G. mellonella with increasing the treatment time. Which was greatly remarkable in larvae treated with gamma irradiated S. carpocapsae. Protein bands at different time intervals for all tested treatments revealed the absence of some bands, appearance of new bands and changes in the intensity of the bands. Therefore, it could be concluded that gamma irradiation of S. carpocapsae increased it’s efficacy and can be used in pest control.

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All authers designed the research, conducted the experiments, analyzed the data, wrote, revised and approved the manuscript.

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Correspondence to R. M. Sayed.

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Salem, H.M., Hussein, M.A., Hafez, S.E. et al. Influence of Gamma Irradiated Steinernema carpocapsae on Some Physiological Aspects of Galleria mellonella Larvae. Biol Bull Russ Acad Sci 48, 165–171 (2021).

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