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Expression of Gibberelline Biosynthesis and Catabolism Genes in the Embryos of γ-Irradiated Barley Seeds

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Abstract

The change in the expression of gibberellin biosynthesis (HvGA3ox2) and catabolism (HvGA2ox3) genes in the embryos of γ-irradiated barley seeds at doses of 4–50 Gy was studied over 30 hours of germination. It was shown that transcription activity of the gibberellin catabolism gene HvGA2ox3 was predominantly increased at each stage of germination, except 12 h after the beginning of the experiment. The transcriptional activity of the gibberellin biosynthesis gene HvGA3ox2 was reduced during the early stages of the observation period (after 6 and 12 hours) and increased after irradiation during the later stages (after 24 and 30 hours). The peak of the transcriptional activity of the genes studied was noted in the embryos of both control and irradiated seeds 18 hours after the onset of germination. At a dose of 20 Gy, the gibberellin biosynthesis gene transcript concentration (HvGa3ox2) was maximal and exceeded the gibberellin catabolism gene transcript concentration (HvGa2ox3) by more than an order of magnitude.

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Authors and Affiliations

  1. Russian Institute of Radiology and Agroecology, Obninsk, Russia

    S. V. Bitarishvili, V. S. Bondarenko & S. A. Geras’kin

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  1. S. V. Bitarishvili
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  2. V. S. Bondarenko
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  3. S. A. Geras’kin
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Correspondence to S. V. Bitarishvili.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Bibov

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Bitarishvili, S.V., Bondarenko, V.S. & Geras’kin, S.A. Expression of Gibberelline Biosynthesis and Catabolism Genes in the Embryos of γ-Irradiated Barley Seeds. Biol Bull Russ Acad Sci 47, 1558–1563 (2020). https://doi.org/10.1134/S1062359020110059

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  • DOI: https://doi.org/10.1134/S1062359020110059

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