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Cryoprotective Properties of the Polysaccharide Fraction of the Mushroom Hericium erinaceus BP 16

  • CELL BIOLOGY
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Abstract

The natural isolate of xylotrophic basidiomycetes Hericium erinaceus BP 16 was cultivated artificially to produce fruiting bodies from which the polysaccharide-containing fraction (PF) was isolated by extraction with hot water (70°C), followed by precipitation with 96% ethanol. Residues of rhamnose, fructose, xylose, arabinose, mannose, glucose, and galactose in the ratio of 7 : 8 : 1 : 19 : 14 : 26 : 27, respectively, were identified in the composition of the PF carbohydrate chains. The effect of adding PF to aqueous solutions of cryoprotectants during water freezing in human venous blood during its cryopreservation has been demonstrated. It was found that the presence of PF (0.25%) in a glycerin-containing (3.5%) preservative ensures the safety of human nuclear blood cells at −20 and −80°C, which indicated the cryoprotective activity of PF H. erinaceus BP 16. Thus, this property of PF may be used in the development of new substances to freeze biological samples.

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Funding

This study was performed within the framework of research project no. 0767-2018-0012 “Study of the Potential Polyfunctional Effects of Micellar Microorganisms in Regional Soil Types for the Creation of New Substances That Can Increase the Adaptability and Environmental Safety of Plant Cultivation and Protection of the Environment from Pollution” and project no. 0415-2016-003 “The Effect of Pectins on the Functional Integrity of Cells during Freezing.”

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Authors and Affiliations

  1. Rudniskii Northeast Federal Agricultural Research Center, 610007, Kirov, Russia

    I. G. Shirokikh, A. A. Shirokikh & Ja. I. Nazarova

  2. Institute of Physiology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982, Syktyvkar, Russia

    T. V. Polezhaeva, A. N. Khudyakov & M. I. Sergushkina

  3. Kirov State Medical University, 610027, Kirov, Russia

    I. G. Paturova

Authors
  1. I. G. Shirokikh
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  2. T. V. Polezhaeva
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  3. A. A. Shirokikh
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  4. A. N. Khudyakov
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  5. M. I. Sergushkina
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  6. Ja. I. Nazarova
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  7. I. G. Paturova
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Correspondence to I. G. Shirokikh.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by I. Matiulko

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Shirokikh, I.G., Polezhaeva, T.V., Shirokikh, A.A. et al. Cryoprotective Properties of the Polysaccharide Fraction of the Mushroom Hericium erinaceus BP 16. Biol Bull Russ Acad Sci 47, 1–6 (2020). https://doi.org/10.1134/S1062359020010124

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  • DOI: https://doi.org/10.1134/S1062359020010124

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