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Use of Dibutyryl Cyclic Adenosine Monophosphate in Sperm Capacitation for in vitro Production of Bovine Embryos

  • CELL BIOLOGY
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Abstract

The opportunity to create a chemically defined system for bovine sperm in vitro capacitation has been performed. 100 µM dibutyryl cyclic adenosine monophosphate (dbcAMP) used as a capacitating agent instead of heparin 10 µg/mL solution was administered in a Tyrode’s protein-free medium for fertilization. In addition, the fertilization medium did not contain glucose or any sperm-activating additives. It has been proven for the first time that spermatozoa capacitated in the in vitro protein-free medium with the use of dbcAMP only are capable of highly efficient in vitro fertilization of the bovine oocytes, which were matured outside the body in a protein-free medium as well. Embryos developed to the 4- to 8-cell stage are chosen as criteria for normal fertilization.

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Authors and Affiliations

  1. All-Russia Research Institute of Animal Physiology, Biochemistry, and Nutrition, Ernst VIZh Federal Research Center, 240913, Borovsk, Kaluga oblast, Russia

    I. G. Smetanina & L. V. Tatarinova

  2. Semenov Institute of Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

    A. S. Krivokharchenko

Authors
  1. I. G. Smetanina
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  2. L. V. Tatarinova
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  3. A. S. Krivokharchenko
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Correspondence to I. G. Smetanina.

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Translated by O. Zhiryakova

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Smetanina, I.G., Tatarinova, L.V. & Krivokharchenko, A.S. Use of Dibutyryl Cyclic Adenosine Monophosphate in Sperm Capacitation for in vitro Production of Bovine Embryos. Biol Bull Russ Acad Sci 46, 327–331 (2019). https://doi.org/10.1134/S1062359019040137

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  • DOI: https://doi.org/10.1134/S1062359019040137

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