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Characterization of Phytase Transgenic Wheat under Salt Stress

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Abstract

Wheat is the major cereal crop. It is grown to meet the demand of food in developing countries but salinity is the serious problem in some countries which are based on agriculture. In the present study fourteen transgenic lines expressing phytase gene were selected using PCR and BASTA leaf paint assay. Different morphological and biochemical analysis that include plant growth, number of spikes, length of spikes, chlorophyll, soluble protein, soluble sugar and proline content were carried out from the leaf of the plants under different stress level of salt. Results showed mostly significant reduction in all the morphological parameters and chlorophyll content with increase in salt concentration while on the other hand in all other parameters, most of them showed significant increment. The result showed that some lines have ability to resist salt stress.

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Authors and Affiliations

  1. Department of Biological Science, Forman Christian College University, Lahore Pakistan (FCCU), Zahoor Elahi Road, Gulberg III, 54600, Lahore, Punjab, Pakistan

    M. Kanwal & A. Maqbool

  2. Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, University Main Road, 38000, Faisalabad, Punjab, Pakistan

    A. Razzaq

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  1. M. Kanwal
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Correspondence to A. Razzaq.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Kanwal, M., Razzaq, A. & Maqbool, A. Characterization of Phytase Transgenic Wheat under Salt Stress. Biol Bull Russ Acad Sci 46, 371–380 (2019). https://doi.org/10.1134/S106235901904006X

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  • DOI: https://doi.org/10.1134/S106235901904006X

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