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Expression and Functional Characteristics of Genes in the Globodera rostochiensis Woll. Potato Cyst Nematode at Different Stages of Its Life Cycle

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Abstract—

The expression of the Ams-1, Pel-1, Eng-1.4, Cle, VAP-1, SPRYSEC-1, Tpx, and Gpx genes was studied in Globodera rostochiensis juveniles at early stages of the life cycle. Low gene activity was registered in juveniles inside the cyst. It was established that the transition to active life is accompanied by transcriptome activation. It was found that when susceptible plants (characterized by slow development of protective reactions) act as a host, gene expression is increased in the juveniles depending on the stage of development; this provides a successful passage of the nematode life cycle. A decreased gene transcription is registered in the juveniles in the case of plants treated with short-term temperature drops (DROP treatment); this can lead to the suppression of nematode development.

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REFERENCES

  1. Beneventi, M.A., Bonfim da Silva, O., Jr., Lisei de Sá, M.E., Firmino, A.A.P., Santos de Amorim, R.M., Albuquerque, É.V.S., Mattar da Silva, M.C., Padilha da Silva, J., de Araújo Campos, M., Lopes, M.J.C., Togawa, R.C., Pappas, G.J., Jr., and Grossi-de-Sa, M.F., Transcription profile of soybean-root-knot nematode interaction reveals a key role of phythormones in the resistance reaction, BMC Genomics, 2013, vol. 14, pp. 1–17. http://www.biomedcentral.com/1471-2164/14/322.

    Article  CAS  Google Scholar 

  2. Bird, D.M., Mechanisms of the Meloidogyne-host interaction, in Nematology: From Molecule to Ecosystem, Dundee, UK: Eur. Soc. Nematologists, 1992, pp. 51–59.

    Google Scholar 

  3. Bird, D.M., Clifton, S.W., Kepler, T., Kieber, J.J., Thorne, J., and Opperman, C.H., Genomic dissection of a nematode-plant interaction: a tool to study plant biology, Plant Physiol., 2002, vol. 129, pp. 394–395.

    Article  CAS  Google Scholar 

  4. Bleve-Zacheo, T., Melillo, M.T., and Zacheo, G., Ultrastructural response of potato roots resistant to cyst nematode Globodera rostochiensis pathotype Ro1, Rev. Nematol., 1990, vol. 13, no. 1, pp. 29–36.

    Google Scholar 

  5. Byrne, J., Twomey, U., Maher, N., and Jones, P.W., Detection of hatching inhibitors and hatching factor stimulants for golden potato cyst nematode, Globodera rostochiensis, in potato root leachate, Ann. Appl. Biol., 1998, vol. 132, no. 3, pp. 463–472.

    Article  CAS  Google Scholar 

  6. Byrne, J.T., Maher, N.J., and Jones, P.W., Comparative responses of Globodera rostochiensis and G. pallida to hatching chemicals, J. Nematol., 2001, vol. 33, no. 4, pp. 195–202.

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Chen, Q., Rehman, S., Smant, G., and Jones, J.T., Functional analysis of pathogenicity proteins of the potato cyst nematode Globodera rostochiensis using RNAi, Mol. Plant Microbe Interact., 2005, vol. 18, no. 7, pp. 621–625.

    Article  CAS  PubMed  Google Scholar 

  8. Cooper, D. and Eleftherianos, I., Parasitic nematode immunomodulatory strategies: recent advances and perspectives, Pathogenes, 2016, vol. 5, no. 58, pp. 1–12.

    Google Scholar 

  9. Cotton, J.A., Lilley, C.J., Jones, L.M., Kikuchi, T., Reid, A.J., Thorpe, P., Tsai, I.J., Beasley, H., Blok, V., Cock, P.J.A., Eves-van den Akker, S., Holroyd, N., Hunt, M., Mantelin, S., Naghra, H., Pain, A., Palomares-Rius, J.E., Zarowiecki, M., Berriman, M., Jones, J.T., and Urwin, P.E., The genome and life-stage specific transcriptomes of Globodera pallida elucidate key aspects of plant parasitism by a cyst nematode, Genome Biol., 2014, vol. 15, pp. 1–17. http://genomebiology.com/2014/15/3/R43.

    Article  CAS  Google Scholar 

  10. Giebel, J., Biochemical mechanisms of plant resistance to nematodes: a review, J. Nematol., 1974, vol. 6, no. 4, pp. 175–184.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Jones, M.G.K., The development and function of plant cells modified by endoparasitic nematodes, in Plant Parasitic Nematodes, Zuckerman, B.M. and Rohde, R.A., Eds., New York: Academic, 1981, vol. 2, pp. 255–279.

    Google Scholar 

  12. Jones, J.T., Perry, R.N., and Johnston, M.R.L., Changes in the ultrastructure of the amphids of the potato cyst nematode, Globodera rostochiensis, during development and infection, Fundam. Appl. Nematol., 1994, vol. 17, no. 4, pp. 369–382.

    Google Scholar 

  13. Kombrink, E. and Schmelzer, E., The hypersensitive response and its role in local and systemic disease resistance, Eur. J. Plant Pathol., 2001, vol. 107, pp. 69–78.

    Article  Google Scholar 

  14. Lavrova, V.V. and Matveeva, E.M., Temperature as management strategy for plant-parasitic nematode Globodera rostochiensis Woll., Russ. J. Nematol., 2015, vol. 23, no. 2, p. 160.

    Google Scholar 

  15. Lavrova, V.V., Sysoeva, M.I., Sherudilo, E.G., Topchieva, L.V., and Matveeva, E.M., Ci7 gene expression in the potato leaves under the action of daily transient temperature drops, Tr. KarNTs Ross. Akad. Nauk, 2011, no. 3, pp. 73–77.

  16. Lavrova, V.V., Sysoeva, M.I., and Matveeva, E.M., Fatty acid composition of potato leaf lipids in a periodic and long-term hypothermia, Tr. KarNTs Ross. Akad. Nauk, 2012, no. 2, pp. 91–96.

  17. Lavrova, V.V., Matveeva, E.M., and Sysoeva, M.I., The effect of low temperature pre-plant treatment of potato tubers under conditions of infection with the potato cyst nematode, S.-Kh. Biol., 2014, no. 1, pp. 98–102.

  18. Lavrova, V.V., Matveeva, E.M., and Zinovieva, S.V., The expression of R genes in genetic and induced resistance to potato cyst nematode Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975, Dokl. Biochem. Biophys., 2015, vol. 464, no. 2, pp. 283–285.

    Article  CAS  PubMed  Google Scholar 

  19. Lavrova, V.V., Matveeva, E.M., and Zinov’eva, S.V., Expression of genes, encoded defense proteins, in potato plants infected with the cyst-forming nematode Globodera rostochiensis (Wollenweber 1923) Behrens, 1975 and modulation of their activity during short-term exposure to low temperatures, Biol. Bull. (Moscow), 2017, vol. 44, no. 2, pp. 128–136.

    Article  CAS  Google Scholar 

  20. Lee, J.H., Lee, J.S., and Ahn, J.H., Ambient temperature signaling in plants: an emerging field in the regulation of flowering time, J. Plant Biol., 2008, vol. 51, no. 5, pp. 321–326.

    Article  CAS  Google Scholar 

  21. Lilley, C.J., Atkinson, H.J., and Urwin, P.E., Molecular aspects of cyst nematodes, Mol. Plant Pathol., 2005, vol. 6, no. 6, pp. 577–588.

    Article  CAS  PubMed  Google Scholar 

  22. Markovskaya, E.F., Sherudilo, E.G., Ripatti, P.O., and Sysoeva, M.I., The role of lipids in the stability of cucumber cotyledons to the permanent and short-term exposure to low hardening temperature, Tr. KarNTs Ross. Akad. Nauk, Ser. Eksp. Biol., 2009, no. 3, pp. 67–74.

  23. Myagi, E.A., Vliyanie bioticheskikh i abioticheskikh faktorov na vyluplenie lichinok kartofel’noi nematody (Effect of Biotic and Abiotic Factors on the Hatching of Potato Cyst Nematode Larvae), Tartu: AN ESSR, 1974.

  24. Palomares-Rius, J.E., Hedley, P.E., Cock, P.J.A., Morris, J.A., Jones, J.T., Vovlas, N., and Blok, V., Comparison of transcript profiles in different life stages of the nematode Globodera pallida under different host potato genotypes, Mol. Pathol., 2012, vol. 13, no. 9, pp. 1120–1134.

    Article  CAS  Google Scholar 

  25. PM 7/40 (3) Globodera rostochiensis and Globodera pallida, Bull. OEPP/EPPO Bull., 2017, vol. 47, no. 2, pp. 174–197.

  26. Robertson, L., Robertson, W.M., Sobczak, M., Helder, J., Tetaud, E., Ariyanayagam, M.R., Ferguson, M.A., Fairlamb, A., and Jones, J.T., Cloning, expression and functional characterisation of a peroxiredoxin from the potato cyst nematode Globodera rostochiensis, Mol. Biochem. Parasitol., 2000, vol. 111, no. 1, pp. 41–49.

    Article  CAS  PubMed  Google Scholar 

  27. Robinson, M.P., Atkinson, H.J., and Perry, R.N., The association and partial of a fluorescent hypersensitive response of potato roots to the potato cyst nematodes Globodera rostochiensis and G. pallida, Rev. Nematol., 1988, vol. 11, pp. 99–107.

    Google Scholar 

  28. Sheridan, J.P., Miller, A.J., and Perry, R.N., Early responses of resistant and susceptible potato roots during invasion by the potato cyst nematode Globodera rostochiensis, J. Exp. Bot., 2004, vol. 55, no. 397, pp. 751–760.

    Article  CAS  PubMed  Google Scholar 

  29. Smant, G., Goverse, A., Stokkermans, J.W.P.G., de Boer, J.M., Pomp, H., Zilverentant, J.F., Overmars, H.A., Helder, J., Schots, A., and Bakker, J., Potato root diffusate-induced secretion of soluble, basic proteins originating from the subventral esophageal glands of potato cyst nematodes, Phytopathology, 1997, vol. 87, pp. 839–845.

    Article  CAS  PubMed  Google Scholar 

  30. Sysoeva, M.I., Lavrova, V.V., Matveeva, E.M., Sherudilo, E.G., and Topchieva, L.V., Cross adaptation of potato plants to low temperatures and potato cyst nematode infestation, Russ. J. Plant Physiol., 2011, vol. 58, no. 6, pp. 999–1004.

    Article  CAS  Google Scholar 

  31. Sysoeva, M.I., Lavrova, V.V., and Matveeva, E.M., Effect of daily transient temperature drops on the contents of photosynthetic pigments in leaves infested with the potato nematode, Tr. KarNTs Ross. Akad. Nauk, 2013, no. 3, pp. 194–199.

  32. Wang, X., Mitchum, M.G., Gao, B., Li, C., Diab, H., Baum, T.J., Hussey, R.S., and Davis, E.L., A parasitism gene from a plant-parasitic nematode with function similar to CLAVATA3/ESR (CLE) of Arabidopsis thaliana, Mol. Plant Pathol., 2005, vol. 6, no. 2, pp. 187–191.

    Article  PubMed  Google Scholar 

  33. Zinovieva, S.V., The relationship of plants and parasitic nematodes: immunobiological aspects, in Trudy Tsentra parazitologii IPEE RAN (Proceedings of the Center for Parasitology, IEE RAS), Moscow: Nauka, 2012, vol. 47, pp. 77–90.

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  1. Institute of Biology, Karelian Research Center, Russian Academy of Sciences, 185910, Petrozavodsk, Russia

    V. V. Seiml-Buchinger & E. M. Matveeva

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  1. V. V. Seiml-Buchinger
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Correspondence to V. V. Seiml-Buchinger.

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Translated by A. Barkhash

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Seiml-Buchinger, V.V., Matveeva, E.M. Expression and Functional Characteristics of Genes in the Globodera rostochiensis Woll. Potato Cyst Nematode at Different Stages of Its Life Cycle. Biol Bull Russ Acad Sci 46, 225–233 (2019). https://doi.org/10.1134/S1062359019030142

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  • DOI: https://doi.org/10.1134/S1062359019030142

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