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Study of Dactylorhiza Seeds (D. baltica and D. maculata) from the Orchid Collection of the Cryobank at Timiryazev Institute of Plant Physiology, Russian Academy of Sciences

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Abstract—

Seeds of two orchid species from the Dactylorhiza genus (D. baltica and D. maculata) collected in Belarus and in Russia and placed for long-term preservation into liquid nitrogen in the cryobank of the Timiryazev Institute of Plant Physiology were studied. The complex quality evaluation of collection specimens was carried out by using the seed viability rating and also by their morphometric and physiology characteristics. It was shown that comprehensive study of seed specimens collected from different habitats at different times may be synchronized by applying a cryopreservation technique and in vitro method for plant material cultivation.

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REFERENCES

  1. Andronova, E.V., Kulikov, P.V., Philippov, E.V., Vasilyeva, V.E., and Batygina, T.B., Problems and perspectives of in vitro seed propagation in orchids of temperate zone, in Embryology of Flowering Plants. Terminology and Concepts. Reproductive Systems, Batygina, T.B., Ed., Enfield, USA: Sci. Publ., 2009, vol. 3, pp. 367–375.

    Google Scholar 

  2. Arditti, J. and Ghani, A.K.A., Numerical and physical properties of orchid seeds and their biological implications: Tansley Rev. no. 110, New Phytol., 2000, vol. 145, pp. 367–421.

    Article  PubMed  Google Scholar 

  3. Aver’yanov, L.V., Dactylorhiza maculate (Orchidaceae) in the territory of the USSR, Bot. Zh., 1982, vol. 67, no. 3, pp. 303–312.

    Google Scholar 

  4. Aver’yanov, L.V., Genus Dactylorhiza (Orchidaceae) in the USSR, Bot. Zh., 1983a, vol. 68, no. 7, pp. 889–895.

    Google Scholar 

  5. Aver’yanov, L.V., Genus Dactylorhiza (Orchidaceae) in the USSR, Bot. Zh., 1983b, vol. 68, no. 9, pp. 1160–1167.

    Google Scholar 

  6. Aver’yanov, L.V., Orchidaceae of Central Russia, Turczaninowia, 2000, vol. 3, no. 1, pp. 30–53.

    Google Scholar 

  7. Bulat, H., Reduktionsvorgänge in lebendem Gewebe, Formazane, Tetrazoliumsalze and ihre Bedeuntung als Redoxindikatoren im ruhenden Samen, Proc. Int. Seed Test. Ass., 1961, vol. 26, pp. 686–696.

    Google Scholar 

  8. Calevo, J., Giovannini, A., Cornara, L., Peccenini, S., and Monroy, F., Orchis patens Desf.: seed morphology of an endangered Mediterranean orchid, Plant Biosyst., 2017, vol. 151, pp. 770–774.

    Article  Google Scholar 

  9. Cottrell, H.J., Tetrazolium salt as a seed germination indicator, Nature (London), 1947, vol. 159, p. 748.

    Article  CAS  PubMed  Google Scholar 

  10. Devos, N., Oh, S.H., Raspé, O., Jacguemart, A.L., and Manos, P.S., Nuclear ribosomal DNA sequence variation and evolution of spotted marsh-orchids (Dactylorhiza maculata group), Mol. Phylogenet. Evol., 2005, vol. 36, no. 3, pp. 568–580.

    Article  CAS  PubMed  Google Scholar 

  11. Devos, N., Raspé, O., Oh, S.H., Tyteca, D., and Jacquemart, A.L., The evolution of Dactylorhiza (Orchidaceae) allotetraploid complex: insights from nrDNA sequences and cpDNA PCR-RFLP data, Mol. Phylogenet. Evol., 2006, vol. 38, no. 3, pp. 767–778.

    Article  CAS  PubMed  Google Scholar 

  12. Efimov, P.G., Study of the genetic polymorphism of Dactylorhiza baltica, D. fuchsia, and D. incarnata (Orchidaceae) from the north-west of the European part of Russia by ISSR, Bot. Zh., 2012, vol. 97, no. 6, pp. 751–761.

    Google Scholar 

  13. Filippov, E.G. and Andronova, E.V., Features of the genetic structure of some species of the genus Dactylorhiza in Russia, in Mater. III Vseros. nauch.-prakt. konf. “Biotekhnologiya kak instrument sokhraneniya bioraznoobraziya rastitel’nogo mira” (Proc. III All-Russia Sci.-Pract. Conf. “Biotechnology as a Tool for Flora Biodiversity Conservation”), Volgograd: AVATARS, 2010, pp. 356–360.

  14. Filippov, E.G. and Andronova, E.V., Features of genetic differentiation of the genera Dactylorhiza, Cypripedium, and Orchis according to isozyme analysis data, in Mater. IX Mezhdunar. nauch. konf. “Okhrana i kul’tivirovanie orkhidei” (Proc. IX Int. Sci. Conf. “Conservation and Cultivation of Orchids”), Moscow: KMK, 2011, pp. 451–461.

  15. Filippov, E.G. and Andronova, E.V., Genetic structure of populations and natural hybridization between Dactylorhiza salina and D. incarnate (Orchidaceae), Russ. J. Genet., 2017, vol. 53, no. 3, pp. 325–337.

    Article  CAS  Google Scholar 

  16. Filippov, E.G., Andronova, E.V., Kozlova, O.N., and Fomenko, T.I., Features of the genetic structure of populations of representatives of the genus Dactylorhiza in the Republic of Belarus according to the isozyme analysis data, in Mater. X Mezhdunar. nauch. konf. “Okhrana i kul’tivirovanie orkhidei” (Proc. X Int. Sci. Conf. “Conservation and Cultivation of Orchids”), Minsk, 2015, pp. 250–255.

  17. Hedrén, M., Genetic differentiation, polyploidization and hybridization in Northern European Dactylorhiza (Orchidaceae), Plant Syst. Evol., 1996, vol. 201, pp. 31–55.

    Article  Google Scholar 

  18. Hedrén, M., Systematics of the Dactylorhiza euxina/incarnate/maculate polyploidy complex (Orchidaceae) in Turkey: evidence from allozyme data, Plant Syst. Evol., 2001, vol. 229, pp. 23–44.

    Article  Google Scholar 

  19. Hedrén, M., Nordstr, S., Pedersen, H., and Ståhlberg, D., Systematics and conservation genetics of Dactylorhiza majalis ssp. elatior (Orchidaceae) on Gotland, Nordic J. Bot., 2012, vol. 30, pp. 257–272.

    Article  Google Scholar 

  20. De Hert, K., Jacquemyn, H., Van Glabeke, S., Roldán-Ruiz, I., Vandepitte, K., Leus, L., and Honnay, O., Patterns of hybridization between diploid and derived allotetraploid species of Dactylorhiza (Orchidaceae) co-occurring in Belgium, Am. J. Bot., 2011, vol. 98, pp. 946–955.

    Article  PubMed  Google Scholar 

  21. De Hert, K., Jacquemyn, H., Van Glabeke, S., Roldán-Ruiz, I., Vandepitte, K., Leus, L., and Honnay, O., Reproductive isolation and hybridization in sympatric populations of three Dactylorhiza species (Orchidaceae) with different ploidy levels, Ann. Bot., 2012, vol. 109, no. 4, pp. 709–720.

    Article  PubMed  Google Scholar 

  22. Jakobsone, G., Belogrudova, I.R., and Daina, M.D., Dactylorhiza baltica in vitro and in vivo, Acta Biol. Univ. Daugavp., 2012, vol. 12, no. 2, pp. 151–165.

    Google Scholar 

  23. Kameswara, R.N., Dulloo, M.E., and Engels, J.M.M., A review of factors that influence the production of quality seed for long-term conservation in genebanks, Genet. Res. Crop Evol., 2017, vol. 64, pp. 1061–1074.

    Article  CAS  Google Scholar 

  24. Kulikov, P.V. and Filippov, E.G., The presence of Dactylorhiza baltica (Klinge) Orlova in the floras of the Urals and Western Siberia, Byull. Mosk. O-va Ispyt. Prir., Otd. Biol., 1999, vol. 104, no. 2, pp. 29–33.

    Google Scholar 

  25. Lakon, G., The topographical tetrazolium method for determining the germinating capacity of seeds, Plant Physiol. Landcaster, 1949, vol. 24, pp. 389–394.

    Article  CAS  Google Scholar 

  26. Levitskaya, G.E., Effect of storage temperature on seeds of wild species. 1. Seeds with forced dormancy and shallow physiological dormancy, Rastit. Resur., 2014, vol. 50, no. 4, pp. 534–548.

    Google Scholar 

  27. Levitskaya, G.E., Effect of storage temperature on seeds of wild species. 2. Seeds with physiological dormancy, as exemplified by the genus Campanula (Campanulaceae), Rastit. Resur., 2015, vol. 51, no. 1, pp. 38–51.

    Google Scholar 

  28. Levitskaya, G.E., Effect of storage temperature on seeds of wild species. 3. Seeds with morphological and morphophysiological dormancy, Rastit. Resur., 2017, vol. 53, no. 1, pp. 39–50.

    Google Scholar 

  29. Li, D.Z. and Pritchard, H.W., The science and economics of ex situ plant conservation, Trends Plant Sci., 2009, vol. 14, pp. 614–621.

    Article  CAS  PubMed  Google Scholar 

  30. Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 1962, vol. 15, no. 4, pp. 473–497.

    Article  CAS  Google Scholar 

  31. Nikishina, T.V., Popov, A.S., Kolomeitseva, G.L., and Golovkin, B.G., Effect of cryoconservation on seed germination of rare tropical orchids, Russ. J. Plant Physiol., 2001, vol. 48, no. 6, pp. 810–815.

    Article  CAS  Google Scholar 

  32. Nikishina, T.V., Vakhrameeva, M.G., Varlygina, T.I., Pavlov, V.N., Shirokov, A.I., Burov, A.V., Popovich, E.A., and Popov, A.S., Cryoconservation of seeds of rare Russian orchids, Dokl. Biol. Sci., 2006, vol. 408, pp. 214–216.

    Article  CAS  PubMed  Google Scholar 

  33. Nikishina, T.V., Popova, E.V., Vakhrameeva, M.G., Varlygina, T.I., Kolomeitseva, G.L., Burov, A.V., Popovich, E.A., Shirokov, A.I., Shumilov, V.Yu., and Popov, A.S., Cryopreservation of seeds and protocorms of rare temperate orchids, Russ. J. Plant Physiol., 2007, vol. 54, no. 1, pp. 121–127.

    Article  CAS  Google Scholar 

  34. Obara-Okeyo, P. and Kako, S., In vitro and in vivo characterization of Cymbidium cultivars by isozyme analysis, J. Horticult. Sci., 1997, vol. 72, no. 2, pp. 263–270.

    Article  CAS  Google Scholar 

  35. Paun, O., Bateman, R.M., Fay, M.F., Hedrěn, M., and Civeyrel, L., Stable epigenetic effect impact adaptation in allopolyploid orchids (Dactylorhiza, Orchidaceae), Mol. Biol. Evol., 2010, vol. 27, no. 11, pp. 2465–2473.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Pillon, Y., Fay, M.F., Hedrén, M., Bateman, R.M., Devay, D.S., Shipunov, A.B., van der Bank, M., and Chase, M.W., Evolution and temporal diversification of western european polyploidy species complexes in Dactylorhiza (Orchidaceae), Taxon, 2006, vol. 56, no. 4, pp. 1185–1208.

    Article  Google Scholar 

  37. Pillon, Y., Fay, M.F., Shipunov, A.B., and Chase, M.W., Species diversity versus phylogenetic diversity: a practical study in the taxonomically difficult genus Dactylorhiza (Orchidaceae), Biol. Conserv., 2007, vol. 129, pp. 4–13.

    Article  Google Scholar 

  38. Plokhinskii, N.A., Matematicheskie metody v biologii (Mathematical Methods in Biology), Moscow: Mosk. Gos. Univ., 1980.

  39. Ponert, J., Vosolsobĕ, S., Kmecová, K., and Lipavská, H., European orchid cultivation—from seed to mature plant, Eur. J. Envir. Sci., 2011, vol. 1, no. 2, pp. 95–107.

    Article  Google Scholar 

  40. Popova, E.V., Kim, H.H., Saxena, P.K., Engelmann, F., and Pritchard, H.W., Frozen beauty: the cryobiotechnology of orchid diversity, Biotech. Adv., 2016, vol. 34, pp. 380–403.

    Article  Google Scholar 

  41. Pritchard, H.W., Liquid nitrogen preservation of terrestrial and epiphytic orchid seeds, CryoLetters, 1984, vol. 5, pp. 295–300.

    Google Scholar 

  42. Pritchard, H.W., The latent life of seeds: from fundamental principles to risk management, Cryobiology, 2007, vol. 55, no. 3, p. 353.

    Article  Google Scholar 

  43. Pritchard, H.W., Kranner, I., and Nadarajan, J., FROZEN PLANeT—the biobanking of plants, Cryobiology, 2010, vol. 61, no. 3, pp. 366–367.

    Article  Google Scholar 

  44. Roberts, E.H., Predicting the storage life of seed, Seed Sci. Technol., 1973, vol. 1, pp. 499–514.

    Google Scholar 

  45. Roberts, E.H., Physiological aspects of ex situ seed conservation, in A Training Manual for Biological Diversity and Genetic Resources, Vijay, P.K. and White, J., London: Comm. Secretariat, 1992, pp. 171–177.

  46. Seaton, P.T., Kendon, J.P., Pritchard, H.W., Puspitaningtyas, D.M., and Marks, T.R., Orchid conservation: the next ten years, Lankesteriana, 2013, vol. 13, nos. 1–2, pp. 93–101.

    Google Scholar 

  47. Shipunov, A.B., Fay, M.F., Pillon, Y., Bateman, R.M., and Chase, M.W., Dactylorhiza (Orchidaceae) in European Russia: combined molecular and morphological analysis, Am. J. Bot., 2004, vol. 91, no. 9, pp. 1419–1426.

    Article  CAS  PubMed  Google Scholar 

  48. Shipunov, A.B., Fay, M.F., and Chase, M.W., Evolution of Dactylorhiza baltica (Orchidaceae) in European Russia: evidence from molecular markers and morphology, Bot. J. Linn. Soc., 2005, vol. 147, no. 3, pp. 257–274.

    Article  Google Scholar 

  49. Ståhlberg, D. and Hedrén, M., Evolutionary history of the Dactylorhiza maculata polyploid complex (Orchidaceae), Biol. J. Linn. Soc., 2010, vol. 101, pp. 503–525.

    Article  Google Scholar 

  50. Tsutsumi, C., Yukawa, T., Lee, N.S., Lee, C.S., and Kato, M., Phylogeny and comparative seed morphology of epiphytic and terrestrial species of Liparis (Orchidaceae) in Japan, J. Plant Res., 2007, vol. 120, pp. 405–412.

    Article  PubMed  Google Scholar 

  51. Vakhrameeva, M.G., Tatarenko, I.V., Varlygina, T.I., Torosyan, G.K., and Zagulski, M.N., Orchids of Russia and Adjacent Countries (Within the Borders of the Former USSR), Koenigstein: A.R.G. Gantner Verlag K.G., 2008.

    Google Scholar 

  52. Vakhrameeva, M.G., Varlygina, T.I., and Tatarenko, I.V., Orkhidnye Rossii (biologiya, ekologiya, okhrana) (Orchids of Russia (Biology, Ecology, and Conservation)), Moscow: KMK, 2014.

  53. Vendrame, W., de Faria, R.T., Sorace, M., and Sahyun, S.A., Orchid cryopreservation, Ciênc. Agrotecnol. Lavras., 2014, vol. 38, pp. 213–229.

    Article  Google Scholar 

  54. Van Waes, J.M. and Debergh, P.C., In vitro germination of some Western European orchids, Physiol. Plant., 1986, vol. 67, no. 2, pp. 253–261.

    Article  CAS  Google Scholar 

  55. Wood, C.B., Pritchard, H.W., and Miller, A.P., Simultaneous preservation of orchid seed and its fungal symbiont using encapsulation-dehydration is dependent on moisture content and storage temperature, CryoLetters, 2000, vol. 21, pp. 125–136.

    CAS  PubMed  Google Scholar 

  56. Yam, T.W., Arditti, J., and Cameron, K.M., “The orchids have been a splendid sport”—an alternative look at Charles Darwin’s contribution to orchid biology, Am. J. Bot., 2009, vol. 96, no. 12, pp. 2128–2154.

    Article  PubMed  Google Scholar 

  57. Yu-Yun Hsiao, Zhao-Jun Pan, Chia-Chi Hsu, Ya-Ping Yang, Yi-Chin Hsu, Yu-Chen Chuang, Hsing-Hui Shih, Wen-Huei Chen, Wen-Chieh Tsai, and Hong-Hwa Chen, Research on orchid biology and biotechnology, Cell Physiol., 2011, vol. 52, no. 9, pp. 1467–1486.

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Funding

This work was supported by the Wildlife Discovery Club of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276, Moscow, Russia

    T. V. Nikishina & O. N. Vysotskaya

  2. State Scientific Institution of Central Botanical Garden, National Academy of Sciences of Belarus, 220012, Minsk, Republic of Belarus

    O. N. Kozlova

  3. Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    G. E. Levitskaya

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  1. T. V. Nikishina
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  2. O. N. Kozlova
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  3. G. E. Levitskaya
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  4. O. N. Vysotskaya
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Correspondence to O. N. Vysotskaya.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Nikishina, T.V., Kozlova, O.N., Levitskaya, G.E. et al. Study of Dactylorhiza Seeds (D. baltica and D. maculata) from the Orchid Collection of the Cryobank at Timiryazev Institute of Plant Physiology, Russian Academy of Sciences. Biol Bull Russ Acad Sci 46, 242–250 (2019). https://doi.org/10.1134/S1062359019030063

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