Biology Bulletin

, Volume 45, Issue 2, pp 171–178 | Cite as

Accumulation of Phenolic Compounds at the Initial Steps of Ontogenesis of Fagopyrum esculentum Plants That Differ in Their Ploidy Levels

  • N. V. Zagoskina
  • V. V. Kazantseva
  • A. N. Fesenko
  • A. V. Shirokova
Plant Physiology


Plants of buckwheat Fagopyrum esculentum possessing diploid and tetraploid genotypes were studied at the initial ontogenetic stages. They were compared in their morphophysiological characteristics, accumulation of phenolic compounds (including their main classes—phenylpropanoids and flavonoids), and activity of L-phenylalanine ammonia-lyase. An apparent resemblance in morphophysiological characteristics of seedlings was found between the two specimens, but diploid plants tended to faster linear growth than tetraploid ones. Differences in the accumulation of phenolic compounds in the hypocotyl and cotyledonous leaves were revealed. In most cases, in the course of seedling growth, the changes in phenylalanine ammonialyase activity did not correlate with the changes in the levels of these secondary metabolites. The effects of gene dosage were established towards accumulation of phenylpropanoids and anthocyanins in hypocotyls of seedlings and flavonoid accumulation in cotyledonous leaves. It is concluded that buckwheat seedlings with a tetraploid genotype have higher capacity than diploid seedlings for biosynthesis of phenolics.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abdallah, S.B., Rabhi, M., Harbaoui, F., Zar-kalai, F., Lacha, M., and Karray-Bouraoui, N., Distribution of phenolic compounds and antioxidant activity between young and old leaves of Carthamus tinctorius L. and their induction by salt stress, Acta Physiol. Plant., 2013, vol. 35, pp. 1161–1169.CrossRefGoogle Scholar
  2. Amelin, A.V., Fesenko, A.N., and Zaikin, V.V., Features of the initial linear growth of the stem and root in buckwheat cultivars at different breeding stages, Zernobobovye Krupyanye Kul’tury, 2013, no. 2 (6), pp. 91–96.Google Scholar
  3. Bidel, L.P.R., Coumans, M., Baissac, Y., Baissac, Y., Doumas, P., and Jay-Allemand, C., Biological activity in plant cells, in Recent Advances in Polyphenol Research, Santos-Buelga, C., Escribano-Bailon, M., and Lattanzio, V., Eds., Oxford, United Kingdom, 2010, vol. 2, pp. 163–205.CrossRefGoogle Scholar
  4. Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.CrossRefPubMedGoogle Scholar
  5. Brillouet, J.-M., On the role of chloroplasts in the polymerization of tannins in tracheophyta, Am. J. Plant Sci., 2015, vol. 6, pp. 1401–1409.CrossRefGoogle Scholar
  6. Brouillard, R., Chassaing, S., Isorez, G., Kueny-Stotz, M., and Figueiredo, P., The visible flavonoidsor anthocyanins: from research to applications, in Recent Advances in Polyphenol Research, Santos-Buelga, C., Escribano-Bailon, M., and Lattanzio, V., Eds., Oxford: United Kindom, 2010, vol. 2, pp. 1–22.Google Scholar
  7. Chacon, I., Riley-Saldana, C., and Gonzalez-Esquinca, A., Secondary metabolites during early development in plants, Phytochem. Rev., 2013, vol. 12, pp. 47–64.CrossRefGoogle Scholar
  8. Charpin, N. and Ellis, B.E., Microspectrophotometric evaluation of rosmarinic acid accumulation in single cultured plant cells, Can. J. Bot., 1984, vol. 62, pp. 2278–2281.CrossRefGoogle Scholar
  9. Cheynier, V., Comte, G., Davis, K.M., Lattanzio, V., and Martens, S., Plant phenolics: recent advances on their biosynthesis, genetics, and ecophysiology, Plant Phys. Biochem., 2013, vol. 72, pp. 1–20.CrossRefGoogle Scholar
  10. Dixon, R.A. and Paiva, N.L., Stress-induced phenylpropanoid metabolism, Plant Cell, 1995, vol. 7, pp. 1085–1097.CrossRefPubMedPubMedCentralGoogle Scholar
  11. Gage, T.B. and Wendei, S.H., Quantitative determination of certain flavonol-3-glycosides, Anal. Chem., 1950, vol. 22, pp. 708–711.CrossRefGoogle Scholar
  12. Higuchi, T., Lignin biochemistry: biosynthesis and biodegradation, Wood Sci. Technol., 1990, vol. 24, pp. 23–63.CrossRefGoogle Scholar
  13. Isachkin, A.V., Solov’ev, A.A., Khanbabaeva, O.E., Bogdanova, V.D., and Zarenkova, E.G., Study of the effect of treatment with aqueous colchicine solution on changes in traits in two garden groups of snapdragon (Antirrhinum majus L.), Izv. Timiryazevsk. S.-Kh. Akad., 2014, no. 4, pp. 5–17.Google Scholar
  14. Jong, F., Hanley, S.J., Beale, M.N., and Karp, A., Characterization on the willow phenylalanine ammonia-lyase (PAL) gene family reveals expression differences compared with poplar, Phytochemistry, 2015, vol. 112, pp. 90–97.CrossRefGoogle Scholar
  15. Khlestkina, E.K., Genes that determine the color of different organs of wheat, Vavilov. Zh. Genet. Selekts., 2012, vol. 16, pp. 202–216.Google Scholar
  16. Klejdus, B., Kovácik, J., and Babula, P., PAL inhibitor evokes different responses in two Hypericum species, Plant Physiol. Biochem., 2013, vol. 63, pp. 82–88.CrossRefPubMedGoogle Scholar
  17. Koyama, M., Nakamura, C., and Nakamura, K., Changes in phenols contents from buckwheat sprouts during growth stage, J. Food Sci. Technol., 2013, vol. 50, no. 1, pp. 86–93.CrossRefPubMedGoogle Scholar
  18. Kurkin, V.A., Farmakognoziya (Pharmacognosy), Samara: Ofort, 2007.Google Scholar
  19. Kurkin, V.A. and Vel’myaikina, E.I., Development of methods of qualitative and quantitative analysis of Echinacea purpurea syrup, Farmatsiya, 2011, no. 7, pp. 10–12.Google Scholar
  20. Li, X., Kim, J.K., Park, S.-Y., Zhao, S., Kim, J.B., Lee, S., and Pak, S.U., Comparative analysis of flavonoids and polar metabolite profiling of tanno-original and tanno-high rutin buckwheat, J. Agric. Food Chem., 2014, vol. 62, pp. 2701–2708.CrossRefPubMedGoogle Scholar
  21. Manzhulin, A.V., Makovetskii, A.F., Terent’eva, E.V., and Voronin, P.Yu., The relationship between the mesostructure and photosynthetic activity of leaves in di- and tetraploid buckwheat genotypes, Fiziol. Rast., 1991, vol. 38, pp. 457–464.Google Scholar
  22. Marakaev, O.A., Celebrowsky, M.V., Nikolaeva, T.N., and Zagoskina, N.V., Some aspects of underground organs of spotleaf Orchis growth and phenolic compounds accumulation at the generative stage of ontogenesis, Biol. Bull. (Moscow), 2013, vol. 40, no. 3, pp. 281–289.CrossRefGoogle Scholar
  23. Margna, U.V., Vzaimosvyaz’ biosinteza flavonoidov s pervichnym metabolizmom rastenii (Correlation of Flavonoid Biosynthesis with Primary Plant Metabolism), Itogi nauki i tekhniki, Ser. Biol. Khim. (Advances in Science and Technology, Ser. Biol. Chem.), Moscow: VINITI AN SSSR, 1990, vol. 33.Google Scholar
  24. Martynenko, G.E., Fesenko, N.V., Fesenko, A.N., and Gurinovich, I.A., Creating a cold-resistant determinant buckwheat cultivar Devyatka, Vestn. OrelGAU, 2010, no. 4, pp. 85–87.Google Scholar
  25. Mokronosov, A.T., Fotosinteticheskaya funktsiya i tselostnost’ rastitel’nogo organizma (The Photosynthetic Function and Integrity of Plant), 42-e Timiryazevskoe chtenie (The 42nd Timiryazev Memorial Lectures), Moscow: Nauka, 1983.Google Scholar
  26. Murav’eva, D.A., Bubenchikova, V.N., and Belikov, V.V., Spectrophotometric determination of the sum of anthocyanins in the flowers of blue cornflower, Farmakologiya, 1987, vol. 36, pp. 28–29.Google Scholar
  27. Nosov, A.M., Secondary metabolism, in Fiziologiya rastenii (Plant Physiology), Ermakov, I.P., Ed., Moscow: Akademiya, 2005, pp. 588–620.Google Scholar
  28. Olenichenko, N.A. and Zagoskina, N.V., Response of winter wheat to cold: production of phenolic compounds and L-phenylalanine ammonia lyase activity, Appl. Biochem. Microbiol., 2005, vol. 41, pp. 600–603.CrossRefGoogle Scholar
  29. Olsen, K.M., Lea, U.S., Slimestad, R., Verheul, M., and Lillo, C., Differential expression of four Arabidopsis PAL genes; PAL1 and PAL2 have functional specialization in abiotic environmental-triggered flavonoid synthesis, Plant Physiol., 2008, vol. 165, pp. 1491–1499.CrossRefGoogle Scholar
  30. Oomah, B.D. and Mazza, G., Flavonoids and antioxidative activities in buckwheat, J. Agric. Food Chem., 1996, vol. 44, pp. 1746–1750.CrossRefGoogle Scholar
  31. Polekhina, N.N. and Pavlovskaya, N.E., Dynamics of accumulation of biochemical compounds with antioxidant activity in various buckwheat organs during ontogenesis, Fundam. Issled., 2013, no. 10, pp. 357–361.Google Scholar
  32. Rat’kin, A.V., Zaprometov, M.N., Andreev, V.S., and Evdokimova, L.I., Study of biosynthesis of anthocyanidins and flavonols in flowers of sweet pea (Lathyrus odoratus L.), Zh. Obshch. Biol., 1980, vol. 41, pp. 685–699.Google Scholar
  33. Rogozhin, V.V. and Rogozhina, T.V., Praktikum po fiziologii i biokhimii rastenii (A Practical Course in Plant Physiology and Biochemistry), St. Petersburg: Giord, 2013.Google Scholar
  34. Sakharov, B.B., Frolova, S.L., and Mansurova, V.V., Tetraploidy in cultural buckwheat (Fagopyrum esculentum), Dokl. Akad. Nauk SSSR, 1944, vol. 43, pp. 223–227.Google Scholar
  35. Sanwal, S.K., Rai, N., Singh, J., and Buragohain, J., Antioxidant phytochemicals and gingerol content in diploid and tetraploid clones of ginger (Zingiber officinale Roscoe), Sci. Horticult., 2010, vol. 124, pp. 280–285.CrossRefGoogle Scholar
  36. Shipilova, S.V. and Zaprometov, M.N., Phenylalanine ammonia-lyase and formation of catechins in the tea plant, Fiziol. Rast., 1977, vol. 24, pp. 803–809.Google Scholar
  37. Tarakhovskii, Yu.S., Kim, Yu.A., Abdrasilov, B.S., and Muzafarov, E.N., Flavonoidy: biokhimiya, biofizika, meditsina (Flavonoids: Biochemistry, Biophysics, and Medicine), Moscow: Pushchino, 2013.Google Scholar
  38. Tuominen, A., Defensive strategies in Geranium sylvaticum. Pt 2: Roles of water-soluble tannins, flavonoids and phenolic acids against natural enemies, Phytochemistry, 2013, vol. 95, pp. 408–420.CrossRefPubMedGoogle Scholar
  39. Vogt, T., Phenylpropanoid biosynthesis, Mol. Plant, 2010, vol. 3, pp. 2–20.CrossRefPubMedGoogle Scholar
  40. Volynets, A.P., Fenol’nye soedineniya v zhiznedeyatel’nosti rastenii (Phenolic Compounds in Plant Life Activity), Minsk: Belaruskaya navuka, 2013.Google Scholar
  41. Vysochina, G.I., Evolution and phylogenetic relationships of genera in the family Polygonaceae (buckwheats) in connection with the biogenesis of phenolic compounds, Rastit. Mir Aziatskoi Rossii, 2008, no. 2, pp. 1–8.Google Scholar
  42. Winkel-Shirley, B., Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology, Plant Physiol., 2001, vol. 126, no. 2, pp. 485–493.PubMedGoogle Scholar
  43. Yakimenko, A.F., Grechikha (Buckwheat), Moscow: Kolos, 1982.Google Scholar
  44. Zagoskina, N.V., Fernando, S.Ch., Fedoseeva, V.G., Azarenkova, N.D., and Zaprometov, M.N., On the ability of diploid and polyploid varieties of tea plants to produce phenolic compounds, S.-Kh. Biol., 1994, no. 1, pp. 117–119.Google Scholar
  45. Zagoskina, N.V., Olenichenko, N.A., Chzhou Yun’vei, and Zhivukhina, E.A., Formation of phenolic compounds in various cultivars of wheat (Triticum aestivum L.), Appl. Biochem. Microbiol., 2005, vol. 41, pp. 99–102.CrossRefGoogle Scholar
  46. Zaman, K., Phenolic compounds and phenylalanine ammonia lyase activity in two soybean (Glycine max L. cv. Mandarin) cell lines that differ in their ploidy levels, Acta Soc. Bot. Pol., 1988, vol. 57, pp. 177–183.CrossRefGoogle Scholar
  47. Zaprometov, M.N., Phenolic compounds and methods of their study, in Biokhimicheskie metody v fiziologii rastenii (Biochemical Methods in Plant Physiology), Moscow: Nauka, 1971, pp. 185–197.Google Scholar
  48. Zaprometov, M.N., Fenol’nye soedineniya. Rasprostranenie, metabolizm i funktsii v rasteniyakh (Phenolic Compounds. Distribution, Metabolism, and Functions in Plants), Moscow: Nauka, 1993.Google Scholar
  49. Zaprometov, M.N. and Nikolaeva, T.N., Chloroplasts isolated from kidney bean leaves are capable of phenolic compound biosynthesis, Russ. J. Plant Physiol., 2003, vol. 50, pp. 623–626.CrossRefGoogle Scholar
  50. Zhurbitskii, Z.I. and Il’in, M.V., Teoriya i praktika vegetatsionnogo metoda (Theory and Practice of Vegetative Method), Moscow: Nauka, 1968.Google Scholar
  51. Zucker, M., Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue, Plant Physiol., 1965, vol. 40, pp. 779–784.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • N. V. Zagoskina
    • 1
  • V. V. Kazantseva
    • 1
  • A. N. Fesenko
    • 2
  • A. V. Shirokova
    • 3
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.All-Russia Research Institute of Grain Legumes and Groats CropsOrelRussia
  3. 3.Koltsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia

Personalised recommendations