Abstract
The activity and spectrum of proteinases in the intestines of host fishes change upon infestation with cestodes. Serine proteinases are found to make a greater contribution to the total proteolytic activity. The reduction of proteolytic activity is associated with adsorption of the enzymes of the host on the surface of cestodes, and the increase in the activity is caused by the injury of the intestinal mucosa by the attachment apparatuses of cestodes. The inhibition of proteainase activity indicates the possible participation of microbiota enzymes in protein hydrolyses.
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Alarcón, F.J., Martínez, T.F., Barranco, P., Cabello, T., Díaz, M., and Moyano, F.J., Digestive proteases during development of larvae of red palm weevil, Rhynchophorus errugineus (Olivier, 1790) (Coleoptera: Curculionidae), Insect Biochem. Mol. Biol., 2002, vol. 32, pp. 265–274.
Chakrabarti, R. and Rathore, R.M., Ontogenetic changes in the digestive enzyme patterns and characterization of proteases in Indian major carp Cirrhinus mrigala, Aqucult. Nutrit., 2010, vol. 16, no. 6, pp. 569–581.
Davydov, V.G., Comparative study of responses of fish intestinal tissues at different ways of attaching of tapeworms, in Biologiya i ekologiya vodnykh organizmov (Biology and Ecology of Aquatic Organisms), Leningrad: Nauka, 1986, pp. 218–227.
Deguara, S., Jauncey, K., and Agius, C., Enzyme activities and pH variations in the digestive tract of gilthead sea bream, J. Fish. Biol., 2003, vol. 62, pp. 1033–1043.
Dimes, L.E., Garcia-Carreno, F.L., and Haard, N.F., Estimation of protein digestibility III. Studies on the digestive enzymes from the pyloric ceca of rainbow trout and salmon, Comp. Biochem. Physiol., 1994, vol. 109A, no. 2, pp. 349–360.
Eshel, A., Lindner, P., Smirnoff, P., Newton, S., and Harpaz, S., Comparative study of proteolytic enzymes in the digestive tracts of the European sea bass and hybrid striped bass reared in freshwater, Comp. Biochem. Physiol., 1993, vol. 106A, no. 4, pp. 621–634.
García-Carreño, F.L., Albuquerque-Cavalcanti, C., Navarrete del Toroa, M.A., and Zaniboni-Filho, E., Digestive proteinases of Brycon orbignyanus (Characidae, Teleostei): characteristics and effects of protein quality, Comp. Biochem. Physiol., 2002, vol. 132B, pp. 343–352.
Izvekova, G.I. and Kuklina, M.M., Infection with cestodes and activity of digestive hydrolases of vertebrate hosts, Usp. Sovrem. Biol., 2014, vol. 134, no. 3, pp. 304–315.
Izvekova, G.I. and Solov’ev, M.M., Activity of fish digestive hydrolases during infection with cestodes, Usp. Sovrem. Biol., 2012, vol. 132, no. 6, pp. 601–610.
Izvekova, G.I., Izvekov, E.I., and Plotnikov, A.O., Symbiotic microflora in fishes of different ecological groups, Biol. Bull. (Moscow), 2007, vol. 34, no. 6, pp. 610–618.
Izvekova, G.I., Solovyev, M.M., and Izvekov, E.I., Effect of Caryophyllaeus laticeps (Cestoda, Caryophyllidea) upon activity of digestive enzymes in bream, Biol. Bull. (Moscow), 2011, vol. 38, no. 1, pp. 50–57.
Jónás, E., Rágyanszki, M., Oláh, J., and Boross, L., Proteolytic digestive enzymes of carnivorous (Silurus glanis L.), herbivorous (Hypuphthalmichthys molitrix Val.) and omnivorous (Cyprinus carpio L.) fishes, Aquaculture, 1983, vol. 30, pp. 145–154.
Kashinskaya, E.N., Sukhanova, E.V., Solov’ev, M.M., Izvekova, G.I., and Glupov, V.V., Diversity of microbial communities of the intestinal mucosa and intestinal contents of fish from Lake Chany (Western Siberia), Inland Water Biol., 2014, vol. 7, no. 2, pp. 172–177.
Kumar, S., García-Carreño, F.L., Chakrabarti, R., Toro, M.A.N., and Córdova-Murueta, J.H., Digestive proteases of three carps Catla catla, Labeo rohita, and Hypophthalmichthys molitrix: partial characterization and protein hydrolysis efficiency, Aquacult. Nutrit., 2007, vol. 13, pp. 381–388.
Kurovskaya, L.Ya., The coupling of digestive processes in the Bothriocephalus acheilognathi–carp system, Parazitologiya, 1991, vol. 25, no. 5, pp. 441–449.
Lundstedt, L.M., Melo, J.F.B., and Moraes, G., Digestive enzymes and metabolic profile of Pseudoplatystoma orruscans (Teleostei: Siluriformes) in response to diet composition, Comp. Biochem. Physiol., 2004, vol. 137B, pp. 331–339.
Munilla-Morán, R. and Saborido-Rey, F., Digestive enzymes in marine species. I. Proteinase activities in gut from redfish (Sebastes mentella), seabream (Sparus aurata) and turbot (Scophthalmus maximus), Comp. Biochem. Physiol., 1996, vol. 113B, no. 2, pp. 395–402.
Natalia, Y., Hashim, R., Ali, A., and Chong, A., Characterization of digestive enzymes in a carnivorous ornamental fish, the Asian bony tongue Scleropages formosus (Osteoglossidae), Aquaculture, 2004, vol. 233, pp. 305–320.
Nomenklatura fermentov (Enzyme Nomenclature), Braunshtein, A.E., Ed., Moscow: VINITI, 1979.
Pappas, P.W. and Read, C.P., Trypsin inactivation by intact Hymenolepis diminuta, J. Parasitol., 1972, vol. 58, pp. 864–871.
Pronina, S.V. and Pronin, N.M., Vzaimootnosheniya v sistemakh gel’minty–ryby (na tkanevom, organnom i organizmennom urovnyakh) (Relationships in the Helminths–Fish Systems (at the Tissue, Organ, and Organism Levels)), Moscow: Nauka, 1988.
Siringan, P., Raksakulthai, N., and Yongsawatdigul, J., Autolytic activity and biochemical characteristics of endogenous proteinases in Indian anchovy (Stolephorus indicus), Food Chem., 2006, vol. 98, pp. 678–684.
Sklan, D., Prag, T., and Lupatsch, I., Structure and function of the small intestine of the tilapia Oreochromis niloticus × Oreochromis aureus (Teleostei, Cichlidae), Aquacult. Res., 2004, vol. 35, pp. 350–357.
Solovyev, M.M., Kashinskaya, E.N., Izvekova, G.I., Gisbert, E., and Glupov, V.V., Feeding habits and ontogenic changes in digestive enzyme patterns in five freshwater teleosts, J. Fish. Biol., 2014, vol. 85, no. 5, pp. 1395–1412.
Ugolev, A.M. and Kuzmina, V.V., Distribution of activity of digestive hydrolases in the epithelial, submucosal, and muscular-serous layers of fish intestines, Dokl. Ross. Akad. Nauk, 1992, vol. 326, no. 3, pp. 566–569.
Ugolev, A.M. and Kuz’mina, V.V., Pishchevaritel’nye protsessy i adaptatsii u ryb (Digestive Processes and the Adaptation of Fish), St. Petersburg: Gidrometeoizdat, 1993.
Vysotskaya, R.U. and Nemova, N.N., Lizosomy i lizosomal’nye fermenty ryb (Lysosomes and Lysosomal Enzymes of Fish), Moscow: Nauka, 2008.
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Original Russian Text © G.I. Izvekova, M.M. Solovyev, 2016, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2016, No. 2, pp. 182–187.
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Izvekova, G.I., Solovyev, M.M. Characteristics of the effect of cestodes parasitizing the fish intestine on the activity of the host proteinases. Biol Bull Russ Acad Sci 43, 146–151 (2016). https://doi.org/10.1134/S1062359016010076
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DOI: https://doi.org/10.1134/S1062359016010076