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Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp. chinensis) cv. Lastochka

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Abstract

The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2–2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige-Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3 g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants.

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Correspondence to D. V. Shumilina.

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Original Russian Text © D.V. Shumilina, N.A. Shmykova, L.L. Bondareva, T.P. Suprunova, 2015, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2015, No. 4, pp. 368–375.

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Shumilina, D.V., Shmykova, N.A., Bondareva, L.L. et al. Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp. chinensis) cv. Lastochka. Biol Bull Russ Acad Sci 42, 302–309 (2015). https://doi.org/10.1134/S1062359015040135

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