Abstract
Interphase prenucleolar bodies are globular bodies which accumulate in large numbers in the nucleoplasm of cultivated cells after hypotonic treatment and subsequent return to isotonic conditions; detailed studies of the role of these structures in the recovery of the nucleolus have not yet been performed. The limited mobility of interphase pronucleoli within the nucleus has been demonstrated. Exchange of the major nucleolar protein B23 between prenucleolar bodies and the surrounding nucleoplasm, rather than stable binding of this protein to the prenucleolar bodies, has been demonstrated using fluorescence recovery after photobleaching method. Gradual accumulation of B23 in the recovering nucleolus with concomitant disappearance of prenucleolar bodies has been demonstrated.
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Original Russian Text © O.M. Lisitsyna, Y.R. Musinova, M.Y. Shubina, V.Y. Polyakov, E.V. Sheval, 2013, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2013, No. 6, pp. 750–753.
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Lisitsyna, O.M., Musinova, Y.R., Shubina, M.Y. et al. The role of interphase prenucleolar bodies in the recovery of the nucleolar structure after reversible hypotonic treatment. Biol Bull Russ Acad Sci 40, 558–561 (2013). https://doi.org/10.1134/S1062359013060083
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DOI: https://doi.org/10.1134/S1062359013060083