Abstract
Brief exposure of yeasts to low-intensity monochromatic light (400–730 nm) has revealed the effects of photoreactivation and photoprotection of the cells inactivated by medium wave UVB radiation (290–320 nm). The red spectral region with a maximum at 680 nm has been found to be the most active in the initiation of photoreactivation and photoprotection. It has been noted that, according to the regularities investigated, these processes differ fundamentally from the known processes of enzymatic photoreactivation and photoprotection, which have a spectral response limited by, respectively, blue (<450 nm) and near (<380 nm) UV light. The data obtained make possible to consider the observed effects of photoreactivation and photo-protection as the manifestation of functioning of some light-dependent defense system capable of increasing the resistance of cells to UVB radiation.
References
Appenzeller, T., Ozone loss hits us where we live, Science, 1991, vol. 254, p. 645.
Caldwell, M.M., Bornman, J.F., Balaré, C.L., et al., Terrestrial ecosystems, increased solar ultraviolet radiation, and interactions with other climate change factors, Photochem. Photobiol. Sci., 2007, vol. 6, pp. 252–266.
Coohill, T.P., Action spectra again?, Photochem. Photobiol., 1991, vol. 54, pp. 859–870.
Enninga, I.C., Groenendijk, R.T.L., Filon, A.R., et al., The wavelength dependence of U.V.-induced pyrimidine dimmer formation, cell killing and mutation induction in human diploid skin fibroblasts, Carcinogenesis, 1986, vol. 7, pp. 1829–1836.
Fraikin, G.Ya., Pospelov, M.E., and Rubin, L.B., Enhancement of the far-UV lethality in yeast Candida guilliermondii by near-UV post-irradiation, Photochem. Photobiol., 1980, vol. 31, pp. 87–88.
Fraikin, G.Ya., Strakhovskaya, M.G., and Ivanova, E.V., The influence of serotonin on the yield of UV-induced thymine dimers in DNA, Biofizika, 1985, vol. 30, pp. 654–657.
Fraikin, G.Ya., Strakhovskaya, M.G., Ivanova, E.V., and Rubin, A.B., Near-UV activation of enzymatic conversion of 5-hydroxytryptophan to serotonin, Photochem. Photobiol., 1989, vol. 49, pp. 475–477.
Fraikin, G.Ya., Photoprotection by serotonin, in Biological Effects of Light, Holick, M.F. and Kligman, A.M., Eds., Berlin: Walter de Gruyter, 1992, pp. 458–462.
Fraikin, G.Ya., Strakhovskaya, M.G., and Rubin, A.B., The role of membrane-bound porphyrin-type compound as endogenous sensitizer in photodynamic damage to yeast plasma membranes, J. Photochem. Photobiol. B, 1996, vol. 34, pp. 129–135.
Fraikin, G.Ya., Strakhovskaya, M.G., and Rubin, A.B., Light-induced processes of cell protection against photodamage, Biochemistry (Moscow), 2000, vol. 65, no. 6, pp. 737–746.
Jenkins, G.I. and Brown, B.A., UV-B perception and signal transduction, in Light and Plant Development, Whitelam, G.C. and Halliday, K.J., Eds., Oxford: Blackwell Publ., 2007, pp. 155–182.
Kelfkens, G., Gruijl, F.R., and Van der Leun, J.C., Ozone depletion and increase in annual carcinogenic ultraviolet dose, Photochem. Photobiol., 1990, vol. 52, pp. 819–823.
Kevei, E., Schafer, E., and Nagy, F., Light-regulated nucleo-cytoplasmic partioning of phytochromes, J. Exp. Bot., 2007, vol. 58, pp. 3113–3124.
Mitchell, D.L., Jen, J., and Cleaver, J.E., Relative induction of cyclobutane dimers and cytosine photohydrates in DNA irradiated in vitro and in vivo with ultraviolet-c and ultraviolet-b light, Photochem. Photobiol., 1991, vol. 54, pp. 41–746.
Sancar, G.B., DNA photolyases: physical properties, action mechanism, and roles in dark repair, Mutat. Res., 1990, vol. 236, pp. 147–160.
Sancar, A., Lindsey-Boltz, L.A., Unsal-Kacmaz, K., and Linn, S., Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints, Annu. Rev. Biochem., 2004, vol. 73, pp. 39–85.
Sancar, A., Structure and function of photolyase and in vivo enzymology: 50th anniversary, J. Biol. Chem., 2008, vol. 283, pp. 32153–32157.
Sarachek, A. and Bish, J.T., Some phylogenetic implication of action spectra for photoreactivation of ultraviolet-inactivated yeasts, Arch. Microbiol., 1975, vol. 104, pp. 73–75.
Strakhovskaya, M.G., Ivanova, E.V., and Fraikin, G.Ya., The stimulatory effect of serotonin on the growth of the yeast Candida guilliermondii and Streptococcus faecalis, Mikrobiologiya, 1993, vol. 62, pp. 46–49.
Tyrrell, R.M., Keyse, S.M., and Moraes, E.C., Cellular defense against UVA (320-380 nm) and UVB (290-320 nm) radiations, in Photobiology: The Science and Its Applications, Ricklis, E.N., Ed., New York: Plenum Press, 1991, pp. 861–871.
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Original Russian Text © G.Ya. Fraikin, N.S. Belenikina, E.V. Pinyaskina, A.B. Rubin, 2013, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2013, No. 6, pp. 754–759.
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Fraikin, G.Y., Belenikina, N.S., Pinyaskina, E.V. et al. New photo-induced effects of reactivation and protection of yeast cells under lethal UVB radiation. Biol Bull Russ Acad Sci 40, 562–566 (2013). https://doi.org/10.1134/S1062359013060058
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DOI: https://doi.org/10.1134/S1062359013060058