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A comparative analysis of karyotypes of three species of Macleaya—producers of a complex of isoquinoline alkaloids

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Abstract

Using a set of methods (C-banding, DAPI-staining, fluorescence hybridization in situ (FISH) with probes of 26S and 5S rDNA, and analysis of meiosis), the first comparative cytogenetic study of three species of Macleaya, producers of complex isoquinoline alkaloids, cordate Macleaya cordata (Willd.) R. Br. (2n = 20), small-fruited Macleaya microcarpa (Maxim.) Fedde (2n = 20) and Macleaya kewensis Turrill (2n = 20), was first carried out. On the basis of morphometric analysis, formulas of karyotypes were made for each species. Species ideograms for M. cordata, M. microcarpa, and M. kewensis were constructed taking into account the polymorphic variants of the C-banding patterns and indicating the location of 26S and 5S rDNA sites. A comparative study revealed that the karyotypes of M. microcarpa and M. kewensis have more in common with each other than with M. cordata. Analysis of meiotic chromosomes suggests of genetic stability of Macleaya genomes. The results of chromosome analysis were used to confirm the close relationship of Macleaya and to clarify their phylogenetic relationships.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 119991, Russia

    T. E. Samatadze, A. V. Amosova, A. V. Zelenin & O. V. Muravenko

  2. Peoples’ Friendship University, ul. Miklukho-Maklaya 6, Moscow, 117198, Russia

    T. E. Samatadze, S. N. Suslina & V. A. Bykov

  3. Russian Institute of Medicinal and Aromatic Plants, Russian Academy of Agricultural Sciences, ul. Greena 7, Moscow, 117216, Russia

    T. N. Zagumennikova, A. N. Tsitsilin & V. A. Bykov

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  1. T. E. Samatadze
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  2. A. V. Amosova
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  3. S. N. Suslina
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  4. T. N. Zagumennikova
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  5. A. N. Tsitsilin
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  6. V. A. Bykov
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  7. A. V. Zelenin
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  8. O. V. Muravenko
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Correspondence to T. E. Samatadze.

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Original Russian Text © T.E. Samatadze, A.V. Amosova, S.N. Suslina, T.N. Zagumennikova, A.N. Tsitsilin, V.A. Bykov, A.V. Zelenin, O.V. Muravenko, 2012, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2012, No. 6, pp. 601–611.

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Samatadze, T.E., Amosova, A.V., Suslina, S.N. et al. A comparative analysis of karyotypes of three species of Macleaya—producers of a complex of isoquinoline alkaloids. Biol Bull Russ Acad Sci 39, 515–524 (2012). https://doi.org/10.1134/S1062359012060106

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