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Involvement of calmodulin in realization of vasoconstrictive effects of serotonin and norepinephrine

  • Animal and Human Physiology
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Abstract

The involvement of calmodulin in adrenergic and serotoninergic regulation of vascular contractility has been studied. Calmodulin inhibitors trifluoperazine and W-13 suppress vasoconstriction of the rat aorta in response to norepinephrine, serotonin, and serotonin 5HT1A and 5HT2A receptor agonists (8-OH-DPAT and DOI, respectively) and do not affect the vasodilatory effect of 5HT1B, 5HT2B, and 5HT4 receptors. The force of aorta contraction in response to 8-OH-DPAT increases after the activation of calcium entry through voltage-gated Ca2+ channels. This effect is not related to nonspecific activation of α1-adrenoceptors, since it is realized in the presence of prazosin. The inhibitor of calmodulin-dependent myosin light chain kinase KN93 decreases the vasoconstrictive response to norepinephrine and serotonin by only 20%. Calmodulin inhibitors slightly decrease aortic constriction in response to endothelin-1, vasopressin, angiotensin II, and KCl. Trifluoperazine does not suppress vasoconstriction induced by the G protein activator AlF −4 . It is assumed that the target of trifluoperazine and W-13 is calmodulin interacting directly with α1-adrenoceptors and serotonin (5HT1A and 5HT2A) receptors.

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Authors and Affiliations

  1. Institute of General Pathology and Pathophysiology, ul. Baltiiskaya 8, Moscow, 125315, Russia

    L. M. Kozhevnikova & P. V. Avdonin

  2. Koltsov Institute of Developmental Biology, ul. Vavilova 26, Moscow, 119334, Russia

    P. V. Avdonin

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  1. L. M. Kozhevnikova
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  2. P. V. Avdonin
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Correspondence to L. M. Kozhevnikova.

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Original Russian Text © L.M. Kozhevnikova, P.V. Avdonin, 2012, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2012, No. 4, pp. 430–437.

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Kozhevnikova, L.M., Avdonin, P.V. Involvement of calmodulin in realization of vasoconstrictive effects of serotonin and norepinephrine. Biol Bull Russ Acad Sci 39, 360–367 (2012). https://doi.org/10.1134/S1062359012030065

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