Abstracts
The population structuring and low genetic diversity of the Manchurian zokor Myospalax psilurus Milne-Edwards, 1874, an East Asian endemic included in the Red List of Russia, were demonstrated. Two separate geographical groups differing in the level of their genetic diversity were found on the territory of the Primorskii krai. The subpopulation located closest to the main area of this species was determined as ancestral. A subspecies differentiation of the Primorskii krai and Transbaikal M. psilurus populations was shown, as was the monophyletic origin of M. psilurus and its high divergence from M. aspalax. The animals from northern localities are recommended for reintroduction in nature under species recovery programs in Primorskii krai.
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Chelomina, G.N., Rozhkovan, K.V., and Ivanov, S.A., Discrimination of Interspecific Hybrids in Natural Populations of Amur Sturgeon Fish by Means of Multilocus RAPD-PCR Markers, Tsitol. Genet., 2008, vol. 42, no. 5, pp. 61–71.
Chelomina, G.N., Spiridonova, L.N., Korablev, V.P., et al., Rare Species of Russian Far East: RAPD-PCR Analysis of Genetic Diversity of Amur Tiger Panthera tigris altaica and Manchuria Zokor Myospalax psilurus espilanus Population, in 4th Eur. Cong. Mammol., Brno, 2003, p. 80.
Chelomina, G.N., Spiridonova, L.N., Kozyrenko, M.V., et al., Estimation of Genetic Polymorphism and Subspecies Diagnostics in the Far-Eastern Leopard Panthera pardus orientalis by Means of RAPD-PCR Analysis of Cellular DNA, Genetika, 1999, vol. 35, pp. 681–687 [Russ. J. Genet. (Engl. Transl.), 1999, vol. 35, no. 5, pp. 573–579].
Chesser, R.K., Genetic Variability within and Among Populations of the Black-Tailed Prairie Dog, Evolution, 1983, vol. 37, pp. 320–331.
Chesser, R.K., Smith, M.H., and Brisbinl, L., Management and Maintenance of Genetic Variability in Endangered Species, Intern. Zoo Yearb., 1980, vol. 20, pp. 146–154.
Gambaryan, P.P., Position of the Genus Myospalax in the System of Rodents. I. Head and Subcutaneous Muscles, Tr. Zool. Inst. Akad. Nauk SSSR, 1982, vol. 115, pp. 3–22.
Gilpin, M. and Soule, M., Minimum Viable Populations: Processes of Species Extinction, Conservation Biology. Sunderland, Massachusetts: Sin. Assoc., 1986, pp. 19–34.
Goodman, S.J., Tamate, H.B., Wilson, R., et al., Bottleneck, Drift and Differentiation: the Population Structure and Demographic History of Sika Deer (Cervus Nippon) in the Japanese Archipelago, Mol. Ecol., 2001, vol. 10, pp. 1357–1370.
Gromov, I.M. and Erbaeva, M.A., Mammals of the Fauna of Russia and Adjacent Territories. Lagomorphs and Rodents, in Opredeliteli po faune Rossii (Identification Guides to the Fauna of Russia), St. Petersburg: Nauka, 1995.
Grosberg, R.K., Characterization of Genetic Structure and Geneologies using RAPD-PCR Markers: a Random Primer for the Novice and Nervous, in Molecular Zoology, Ferraris, J.D. and Palumbi, S.R., Eds., New York: Wiley, 1996, pp. 67–100.
Gurdebeke, S., Maelfait, J.-P., and Backeljau, T., Constructing Allozyme and RAPD Variation in Spider Populations from Patchy Forest Habitats, Genetics, 2003, vol. 119, pp. 27–34.
Jansa, S.A. and Weksler, M., Phylogeny of Muroid Rodents: Relationships within and among Major Lineages As Determined by IRBP Gene Sequences, Mol. Phyl. Evol., 2004, vol. 31, pp. 256–276.
Kenney, J.S., Smith, J.L.D., Starfield, A.M., and McDougal, C.W., The Long-Term Effects of Tiger Pouching on Population Variability, Conserv. Biol., 1994, vol. 9, pp. 1127–1133.
Korablev, V.P. and Pavlenko, M.V., Current State and Protection of the Population of the Transbaikal Zokor Myospalax psilurus the Southern Russian Far East, in Mater. Sib. Zool. Konf. (Proc. Sib. Zool. Conf.), Novosibirsk, 2004, pp. 142–143.
Kostenko, V.A. and Korablev, V.P., The Transbaikal Zokor, in Krasnaya kniga Primorskogo kraya: Zhivotnye (Red Data Book of Primorsky Krai: Animals), Vladivostok: Apel’sin, 2005, pp. 363–365.
Kostenko, V.A., Gryzuny (Rodentia) Dal’nego Vostoka Rossii (Rodents (Rodentia) of the Far East of Russia), Vladivostok: Dal’nauka, 2000.
Kostenko, V.A., The Transbaikal Zokor Myospalax psilurus epsilanus, in Krasnaya kniga Rossii (Red Data Book of Russia), Moscow: AST, 2001, pp. 625–626.
Kulikova, I.V., Chelomina, G.N., and Surmach, S.G., Status and Genetic Diversity of the Black Vulture (Aegipius monachus) Population in Primorsky Krai, in Sovremennye problemy ornitologii v Tsentral’noi Azii i Mongolii (Modern Problems of Ornithology in Central Asia and Mongolia), Ulan-Ude: Buryat. Gos. Univ., 2003, pp. 19–21.
Kuznetsova, M.V. and Kholodova, M.V., The Use of RAPD PCR Analysis for Identification of the Mongolian and Kalmyk Subspecies of the Saiga (Saiga tatarica), in Mater. Nauch. Konf. Pamyati Gregora Mendelya (Proc. Sci. Conf. in Memory of Gregor Mendel), Moscow: MSKhA, 2001, pp. 163–164.
Kuznetsova, M.V., Kholodova, M.V., and Lomov, A.A., The Study of DNA Polymorphism in Kalmykian Saigas by DNA Amplification with Random Primers, in Mater. Konf. “Problema vida i vidoobrazovaniya” (Proc. Conf. “Problem of Species and Speciation”), Tomsk, 2000, p. 76.
Lande, R. and Berrouklaf, J.F., Effective Population Size, Genetic Variation and Their Use in Population Management, in Zhiznesposobnost’ populyatsii (Viability of Populations), Suley, M., Ed., Moscow: Mir, 1989.
Leberg, P.L., Estimating Allelic Richness: Effects of Sample Size and Bottlenecks, Mol. Ecol., 2002, vol. 11, pp. 2445–2449.
Maniatis, T., Fritsch, E. F., and Sambrook, J. Molecular Cloning, Cold Spring Harbor, New York: Cold Spring Harbor Lab. Press, 1982. Translated under the title Metody geneticheskoi inzhenerii Moscow: Mir, 1984.
Martynova, L.Ya., Fomicheva, I.I., and Vorontsov, N.N., Electrophoretic Study of Blood Proteins of Three Zokor Species (Rodentia, Myospalacinae), Zool. Zh., 1977, vol. 56, pp. 1538–1542.
Martynova, L.Ya., Chromosomal Differentiation of Three Zokor Species (Rodentia, Myospalacinae), Zool. Zh., 1976, vol. 55, pp. 1265–1268.
Miller, M.P., Tools for Population Genetics Analyses (TFPGA). 1.3. A Windows Program for Analysis of Allozyme and Molecular Population Genetic Data. Computer Software Distributed by the Authors, 1997.
Musser, G.G. and Carleton, M.D., Family Muridae, in Mammalian Species of the World. A Taxonomic and Geographic Reference, Wilson, D., Reeder, D., and Washington, D.C., Eds., Washington, D.C.: Smiths. Inst. Press, 1993, pp. 501–756.
Norris, R.W., Zhou, K.Y., Zhou, C.Q., et al., The Phylogenetic Position of the Zokors (Myospalacinae) and Comments on the Families of Muroids (Rodentia), Mol. Phyl. Evol., 2004, vol. 31, pp. 972–978.
O’Brien, S.J., A Role for Molecular Genetics in Biological Conservation, Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 5748–5755.
Ognev, S.I., Zveri SSSR i prilezhashchikh stran (Animals of the USSR and Adjacent Countries), vol. 5: Gryzuny (Rodents), Moscow: Akad. Nauk SSSR, 1947, pp. 641–686.
Pavlenko, M.V. and Korablev, V.P., Genetic Differentiation, Taxonomic Position, and the Problem of Boundary Populations of Transbaikal Zokor Myospalax psilurus (Rodentia, Myospalacinae), in Problemy evolyutsii (Problems of Evolution), Vladivostok: Dal’nauka, 2003, vol. V, pp. 167–177.
Pavlinov, I.Ya., Yakhontov, E.L., and Agadzhanyan, A.K., Mlekopitayushchie Evrazii. I. Rodentia: sistematikogeograficheskii spravochnik (Issledovaniya po faune) (Mammals of Eurasia. I. Rodentia: Taxonomic and Geographic Reference (Faunistic Studies)), Rossolimo, O.L., Ed., Moscow: Mosk. Gos. Univ., 1995.
Plyater-Plokhotskii, K.A., Vrednye i poleznye mlekopitayushchie v sel’skom khozyaistve DV kraya (Harmful and Beneficial Mammals in Agriculture of the Far East), Moscow, Dal’giz, 1936.
Ralls, K., Ballou, J.D., and Templeton, A., Estimates of Lethal Equivalents and the Cost of Inbreeding in Mammals, Conserv. Biol., 1988, vol. 2, pp. 185–193.
Raymond, M.L. and Rousset, F., An Exact Test for Population Differentiation, Evolution, 1995, vol. 49, pp. 1280–1283.
Reed, D., Lowe, E.H., Briscoe, D.A., and Frankham, R., Inbreeding and Extinction: Effects of Rate of Inbreeding, Conservat. Gen., 2003, vol. 4, pp. 405–410.
Rohlf, F.J., NTSYS-Pc: Numerical Taxonomy and Multivariate Analysis System, New York: Appl. Biostat, 1993.
Sokal, R.R. and Rohlf, F.J., Biometry, San Francisco: W.H. Freeman, 1995.
Talbot, S.L., Pearce, J.M., Pierson, B.J., et al., Molecular Status of the Dusky Canada Goose (Branta canadensis occidentalis): A Genetic Assessment of a Translocation Effort, Conserv. Genet., 2003, vol. 4, pp. 367–381.
Van de Peer, G. and De Wachter, R., Construction of Evolutionary Distance Trees with TREECON for the Windows: Accounting for Variation in Nucleotide Substitution Rate among Sites, Comput. Appl. Biosci., 1997, vol. 13, pp. 227–230.
Whitehouse, A.M. and Harley, E.H., Post-Bottleneck Genetic Diversity of Elephant Populations in South Africa, Revealed using Microsatellite Analysis, Mol. Ecol., 2001, vol. 10, pp. 2139–2149.
Yeh, F.C., Yang, R.C., Boyle, T.B.J., et al., POPGENE v. 3.2. The User-Friendly Shareware for Population Genetics Analyses, Edmonton: Univ. Alberta, 1999.
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Original Russian Text © G.N. Chelomina, V.P. Korablev, M.V. Pavlenko, 2011, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2011, No. 3, pp. 273–282.
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Chelomina, G.N., Korablev, V.P. & Pavlenko, M.V. Genetic diversity and phylogenetic relationships of the Manchurian zokor Myospalax psilurus (Rodentia, Muridae) according to RAPD-PCR analysis. Biol Bull Russ Acad Sci 38, 222–230 (2011). https://doi.org/10.1134/S1062359011030034
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DOI: https://doi.org/10.1134/S1062359011030034