Abstract
The possibility of interaction of recombinant proteins of human repair DNA polymerase β with proofreading 3′ → 5′-exonucleases TREX1 and TREX2 was investigated in vitro for the first time. The results of gel filtration analysis show the formation of a complex between 3′ → 5′-exonucleases mTREX1 and hTREX2 and DNA polymerase β. DNA polymerase activity is shown to increase four-fold in the presence of 3′ → 5′-exonuclease TREX2. The experiments with the use of immunodot and Western blot assays on the binding of DNA-polymerase β with 3′ → 5′-exonucleases TREX1 and TREX2 immobilized on a nitrocellulose membrane provided additional evidence on the direct association of the above proteins in complexes.
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Original Russian Text © N.V. Belyakova, O.K. Legina, N.L. Ronzhina, I.V. Shevelev, V.M. Krutiakov, 2010, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2010, No. 5, pp. 547–553.
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Belyakova, N.V., Legina, O.K., Ronzhina, N.L. et al. Investigation of the interaction of repair DNA polymerase β and autonomous 3′ → 5′-exonucleases TREX1 and TREX2. Biol Bull Russ Acad Sci 37, 464–470 (2010). https://doi.org/10.1134/S1062359010050043
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DOI: https://doi.org/10.1134/S1062359010050043